Abstract:

Objective To study the role of gene chip technology in the identification and drug resistance of Mycobacterium. Methods The sputum specimens of 680 patients diagnosed as tuberculosis（positive acid fast stain） were collected from January 2014 to December 2016 in the Third People's Hospital of Yichang. Mycobacterium was identified by gene chip technology and culturing. The drug resistance of Mycobacterium tuberculosis（MTB） to rifampin and isoniazid was determined by gene chip technology and culturing. Culturing was used as reference method,and the consistency rate was evaluated. The drug susceptibility test was used as a gold standard for calculating the sensitivity,specificity and consistency rate of gene chip technology. Results A total of 31 isolates of nontuberculous Mycobacterium（NTM） were determined by gene chip technology,and 32 isolates of NTM were determined by culturing. The consistency rate was 96.88%. Compared with drug susceptibility test,the sensitivity of gene chip technology for MTB to rifampin was 89.81%,the specificity was 93.55%,the consistency rate was 92.80%,and the Kappa value was 0.82（P>0.05）. The sensitivity of gene chip technology for MTB to isoniazid was 83.06%,the specificity was 91.39%,the consistency rate was 89.48%,and the Kappa value was 0.73（P>0.05）. Conclusions Gene chip technology is an effective method for accurately screening NTM. Gene chip technology and culturing have high sensitivity and specificity. There is a good consistency between gene chip technology and culturing and drug susceptibility test. Gene chip technology is effective,rapid and safe.

Key words: Gene chip, Mycobacterium tuberculosis, Nontuberculous Mycobacterium, Rifampin, Isoniazid, Drug resistance