Th1/Th2 cytokines secreted by phytohaemagglutinin-stimulated peripheral blood mononuclear cells for the diagnosis of Alzheimer's disease

doi:10.3969/j.issn.1673-8640.2018.04.004

Abstract

Abstract:

Objective To investigate the roles of cytokines,serum cytokines and blood lipid secreted by phytohaemagglutinin（PHA）-stimulated peripheral blood mononuclear cells（PBMC） for the diagnosis of Alzheimer's disease（AD）. Methods A total of 100 AD patients and 100 healthy subjects were enrolled,and their blood samples were collected. PBMC was extracted. The levels of culture solution cytokine interleukin（IL）-1 alpha,IL-6,IL-10,tumor necrosis factor alpha（TNF-α） and interferon gamma（IFN-γ）,serum cytokines（sIL-1α）,sIL-6,sIL-10,sTNF-α and sIFN-γ and blood lipid [total cholesterol（TC）,triglyceride（TG）,high-density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C）] levels were determined by enzyme-linked immunosorbent assay（ELISA）. Samples were collected and determined again after 6 weeks. Results The levels of IL-1α and IFN-γ in AD group were higher than those in control group（P<0.05）,while the level of IL-10 in AD group was lower than that in control group（P<0.05）. The levels of sIL-6 and sTNF-α were higher in AD group than those in control group（P<0.05）. The levels of TC,TG and LDL-C in AD group were higher than those in control group（P<0.05）. After 6 weeks,the results of re-sampling were similar those of the first determination. Conclusions Cytokines,serum cytokines and blood lipid secreted by PHA-stimulated PBMC,such as IFN-γ, may be associated with AD. Among them,IFN-γ,sIL-6,sTNF-α,TC,TG and LDL-C may be potential for the diagnosis of AD.

Key words: Cytokine, Phytohaemagglutinin, Peripheral blood mononuclear cell, Alzheimer's disease

CLC Number:

R446.62

DING Bingyu, LIN Ping, LI Qingtian. Th1/Th2 cytokines secreted by phytohaemagglutinin-stimulated peripheral blood mononuclear cells for the diagnosis of Alzheimer's disease[J]. Laboratory Medicine, 2018, 33(4): 290-294.

Figures/Tables 5

References 18

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组别	例数	TC	TG	LDL-C	HDL-C
AD组
AD1	100	6.25±0.77^*	2.20±0.63^*	4.18±0.78^*	1.42±0.41
AD2	100	6.33±1.03^*	2.27±0.41^*	4.32±0.94^*	1.42±0.57
对照组	100	5.27±0.63	1.46±0.69	3.36±0.62	1.47±0.79

组别	例数	TC	TG	LDL-C	HDL-C
AD组
AD1	100	6.25±0.77^*	2.20±0.63^*	4.18±0.78^*	1.42±0.41
AD2	100	6.33±1.03^*	2.27±0.41^*	4.32±0.94^*	1.42±0.57
对照组	100	5.27±0.63	1.46±0.69	3.36±0.62	1.47±0.79

指标	AUC	标准差	P值	95%可信区间
IFN-γ/IL-6比值	0.951	0.023	<0.001	0.905~0.996
IFN-γ/IL-10比值	0.912	0.036	<0.001	0.842~0.982
IFN-γ	0.911	0.037	<0.001	0.838~0.984
IL-1α	0.644	0.082	0.051	0.483~0.805
sIL-6	0.894	0.061	<0.001	0.774~1.000
sTNF-α	0.888	0.064	<0.001	0.761~1.000
TC	0.912	0.042	<0.001	0.829~0.995
TG	0.911	0.044	<0.001	0.825~0.996
LDL-C	0.897	0.049	<0.001	0.801~0.993

指标	AUC	标准差	P值	95%可信区间
IFN-γ/IL-6比值	0.951	0.023	<0.001	0.905~0.996
IFN-γ/IL-10比值	0.912	0.036	<0.001	0.842~0.982
IFN-γ	0.911	0.037	<0.001	0.838~0.984
IL-1α	0.644	0.082	0.051	0.483~0.805
sIL-6	0.894	0.061	<0.001	0.774~1.000
sTNF-α	0.888	0.064	<0.001	0.761~1.000
TC	0.912	0.042	<0.001	0.829~0.995
TG	0.911	0.044	<0.001	0.825~0.996
LDL-C	0.897	0.049	<0.001	0.801~0.993