Expression levels and their roles of serum IL-1β,IL-6,TNF-α,IL-17 and IL-23 of schizophrenia patients

doi:10.3969/j.issn.1673-8640.2018.08.007

Abstract

Abstract:

Objective To investigate the changes of serum interleukin（IL）-1β,IL-6,tumor necrosis factor-alpha（TNF-α）,IL-17 and IL-23 levels in schizophrenia patients,and to evaluate the roles in the auxiliary diagnosis of schizophrenia.Methods A total of 85 patients with schizophrenia were enrolled as case group,and 85 healthy subjects were enrolled as control group. IL-1β,IL-6,TNF-α,IL-17 and IL-23 were determined by enzyme-linked immunosorbent assay（ELISA）. The changes of case group before and after treatment for 3 months were evaluated. The auxiliary diagnosis role of serum cytokines was evaluated by receiver operating characteristic（ROC） curve. Pearson correlation analysis was used to analyze the correlation between the levels of serum cytokines and the positive and negative syndrome scale（PANSS） score.Results Before treatment,serum levels of IL-1β,IL-6,TNF-α,IL-17 and IL-23 in case group were higher than those in control group（P<0.01）. After treatment for 3 months,serum levels of IL-1β,IL-6,TNF-α,IL-17 and IL-23 in case group were lower than those before treatment（P<0.01）,and there was no statistical significance compared with control group（P>0.05）. The areas under ROC curves（AUC） of serum IL-1β,IL-6,TNF-α,IL-17 and IL-23 were 0.723,0.772,0.686,0.685 and 0.679. Serum IL-1β,IL-17 and IL-23 were positively correlated with PANSS total score,positive symptom score,general psychopathology score and supplemental aggression risk subscale score TNF-α was positively correlated with positive symptom score（P<0.05）,and IL-6 was positively correlated with PANSS total score and negative symptom score（P<0.05）.Conclusions The activation of serum cytokines（IL-1β,IL-6,TNF-α,IL-17 and IL-23） exists in schizophrenia patients,serum cytokines play roles in the auxiliary diagnosis of schizophrenia,and these levels are correlated with clinical symptoms.

Key words: Cytokine, Schizophrenia, Auxiliary diagnosis, Clinical symptom

CLC Number:

R446.62

LI Zebing, LI Dong, YANG Xuesong, SUN Zujun, LIN Ping, WANG Feng, LIN Feiran, XU Xiao, XIE Hongtao. Expression levels and their roles of serum IL-1β,IL-6,TNF-α,IL-17 and IL-23 of schizophrenia patients[J]. Laboratory Medicine, 2018, 33(8): 697-701.

Figures/Tables 6

References 13

[1]	王祖承,方贻儒. 精神病学[M]. 上海:上海科技教育出版社,2011:116.
[2]	FILLRMAN S G,WEICKERT T W,LENROOT R K,et al.Elevated peripheral cytokines characterize a subgroup of people with schizophrenia displaying poor verbal fluency and reduced Broca's area volume[J]. Mol Psychiatry,2016,21(8):1090-1098.
[3]	WATANABE Y,SOMEYA T,NAWA H.Cytokine hypothesis of schizophrenia pathogenesis:evidence from human studies and animal models[J]. Psychiatry Clin Neurosci,2010,64(3):217-230.
[4]	EL KISSI Y,SAMOUD S,MTIRAOUI A,et al.Increased interleukin-17 and decreased BAFF serum levels in drug-free acute schizophrenia[J]. Psychiatry Res,2015,225(1-2):58-63.
[5]	KUNZ M,CERESÈR K M,GOI P D,et al. Serum levels of IL-6,IL-10 and TNF-α in patients with bipolar disorder and schizophrenia:differences in pro- and anti-inflammatory balance[J]. Rev Bras Psiquiatr,2011,33(3):268-274.
[6]	MEYER U,FELDON J,YEE B K,et al.A review of the fetal brain cytokine imbalance hypothesis of schizophrenia[J]. Schizophr Bull,2009,35(5):959-972.
[7]	KHANDAKER G M,ZIMBRON J,LEWIS G,et al.Prenatal maternal infection,neurodevelopment and adult schizophrenia:a systematic review of population-based studies[J]. Psychol Med,2013,43(2):239-257.
[8]	宋学勤,陈旭梅,张伟,等. 脂联素及白细胞介素-1β等细胞因子在首发未用药精神分裂症患者中的作用[J]. 中华医学杂志,2013,93(41):3256-3260.
[9]	朱琦玥,李雪,黑钢瑞,等. 首发精神分裂症患者血清中高迁移率族蛋白B1及细胞因子的变化分析[J]. 中华医学杂志,2015,95(47):3818-3822.
[10]	丁彬彬,邬建民,介勇,等. 阿尔茨海默病和血管性痴呆患者血清炎症因子和生化指标的检测及临床意义[J]. 检验医学,2016,31(5):363-367.
[11]	KIM Y K,MYINT A M,LEE B H,et al.Th1,Th2 and Th3 cytokine alteration in schizophrenia[J]. Prog Neuropsychopharmacol Biol Psychiatry,2004,28(7):1129-1134.
[12]	DEBNATH M,BERK M.Th17 pathway-mediated immunopathogenesis of schizophrenia:mechanisms and implications[J]. Schizophr Bull,2014,40(6):1412-1421.
[13]	LI H,ZHANG Q,LI N,et al.Plasma levels of Th17-related cytokines and complement C3 correlated with aggressive behavior in patients with schizophrenia[J]. Psychiatry Res,2016,246:700-706.

组别	例数	IL-1β	IL-6	TNF-α	IL-17	IL-23
病例组	85
治疗前		48.68±32.54^*	38.00±29.31^*	23.09±5.70^*	13.79±6.50^*	30.31±15.90^*
治疗3个月末		34.07±21.44^#	23.73±11.43^#	18.89±6.10^#	10.40±4.45^#	19.64±12.50^#
正常对照组	85	31.44±12.51	21.59±8.44	19.06±5.26	10.59±3.83	22.22±9.15

组别	例数	IL-1β	IL-6	TNF-α	IL-17	IL-23
病例组	85
治疗前		48.68±32.54^*	38.00±29.31^*	23.09±5.70^*	13.79±6.50^*	30.31±15.90^*
治疗3个月末		34.07±21.44^#	23.73±11.43^#	18.89±6.10^#	10.40±4.45^#	19.64±12.50^#
正常对照组	85	31.44±12.51	21.59±8.44	19.06±5.26	10.59±3.83	22.22±9.15

组别	例数	PANSS总分	阳性症状评分	阴性症状评分	一般精神病理学症状评分	攻击危险性评分
治疗前	85	121.07±14.82	27.71±4.92	30.06±4.26	51.78±6.50	11.53±2.15
治疗3个月末	85	88.88±15.19	17.48±4.91	24.24±4.15	38.86±6.66	8.32±1.78
t值		32.354	24.045	14.889	24.792	19.576
P值		0.000	0.000	0.000	0.000	0.000

组别	例数	PANSS总分	阳性症状评分	阴性症状评分	一般精神病理学症状评分	攻击危险性评分
治疗前	85	121.07±14.82	27.71±4.92	30.06±4.26	51.78±6.50	11.53±2.15
治疗3个月末	85	88.88±15.19	17.48±4.91	24.24±4.15	38.86±6.66	8.32±1.78
t值		32.354	24.045	14.889	24.792	19.576
P值		0.000	0.000	0.000	0.000	0.000

项目	最佳临界值（pg/mL）	敏感性（%）	特异性（%）	约登指数	AUC（95%可信区间）	标准误
IL-1β	40.083	56.5	87.1	0.436	0.723（0.644~0.801）	0.040
IL-6	20.037	87.1	56.5	0.436	0.772（0.702~0.841）	0.035
TNF-α	17.115	89.4	36.5	0.259	0.686（0.607~0.764）	0.040
IL-17	11.603	60.0	71.8	0.318	0.685（0.604~0.765）	0.041
IL-23	25.391	56.5	78.8	0.353	0.679（0.598~0.760）	0.041