Regulator T cells and cytokines in peripheral blood of cervical cancer patients with different stages

doi:10.3969/j.issn.1673-8640.2017.09.006

Abstract

Abstract:

Objective To investigate regulator T cell（Treg） ratio and cytokines in peripheral blood of cervical cancer patients with different stages. Methods A total of 80 cervical cancer patients with different stages and 24 healthy females were enrolled. The levels of interleukin（IL）-2,IL-10,transforming growth factor-beta（TGF-β）,cytotoxic T-lymphocyte antigen 4（CTLA-4） and lymphocyte activation gene 3（LAG-3） in serum were determined by enzyme-linked immunosorbent assay（ELISA）,and flow cytometry was used to determine the ratio of Treg in peripheral blood. Results The level of IL-2 in patients with cervical cancer was lower than that in healthy control group（P<0.01）,and decreased with stage increasing. The levels of IL-10 and TGF-β were higher than those in healthy control group（P<0.05）,and increased with stage increasing. The level of CTLA-4 was higher than that in healthy control group（P<0.05）,but there was no statistical significance among different stages（P>0.05）. The level of LAG-3 at stage Ⅲ was higher than that in healthy control group（P<0.05）. The ratio of Treg in cervical cancer patients was higher than that in healthy control group（P<0.05）, and increased with stage increasing. Conclusions The levels of IL-2,IL-10 and TGF-β and Treg ratio in peripheral blood are closely related to the development of cervical cancer. The monitoring and analysis of these indices will help to reveal the immune mechanism of cervical cancer.

Key words: Regulator T cell, Cytokine, Cervical cancer

CLC Number:

R446.62

HUANG Qunhuan, HONG Ling, TAO Chunlin, HUANG Xuhong. Regulator T cells and cytokines in peripheral blood of cervical cancer patients with different stages[J]. Laboratory Medicine, 2017, 32(9): 769-772.

Figures/Tables 2

References 9

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组别	例数	IL-2	IL-10	TGF-β	CTLA-4	LAG-3
宫颈癌组	80
Ⅰ期	12	8.66±1.77^**	11.84±3.02^*	10.77±2.42^*	11.44±1.78^*	12.91±2.38
Ⅱ期	28	7.09±1.09^**	11.97±1.99^*	11.39±2.06^*	12.01±2.63^*	12.05±2.87
Ⅲ期	31	4.80±1.21^**	13.65±3.24^*	13.71±2.96^*	11.96±3.04^*	13.11±3.04^*
Ⅳ期	9	3.91±0.96^**	15.07±4.11^**	14.49±3.20^*	11.78±2.58^*	12.74±2.77
对照组	24	12.23±3.16	9.71±2.21	8.60±1.31	9.06±1.03	11.24±2.52

组别	例数	IL-2	IL-10	TGF-β	CTLA-4	LAG-3
宫颈癌组	80
Ⅰ期	12	8.66±1.77^**	11.84±3.02^*	10.77±2.42^*	11.44±1.78^*	12.91±2.38
Ⅱ期	28	7.09±1.09^**	11.97±1.99^*	11.39±2.06^*	12.01±2.63^*	12.05±2.87
Ⅲ期	31	4.80±1.21^**	13.65±3.24^*	13.71±2.96^*	11.96±3.04^*	13.11±3.04^*
Ⅳ期	9	3.91±0.96^**	15.07±4.11^**	14.49±3.20^*	11.78±2.58^*	12.74±2.77
对照组	24	12.23±3.16	9.71±2.21	8.60±1.31	9.06±1.03	11.24±2.52

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