Establishment of the reference intervals of coagulation function in 0-3-year-old children

doi:10.3969/j.issn.1673-8640.2018.02.003

Abstract

Abstract:

Objective To establish the reference intervals of coagulation function in 0-3-year-old children. Methods Coagulation function was determined in 966 children of 0-3-year-old. The children were required without fever,inflammation,hematological diseases,digestive diseases,respiratory diseases,endocrine diseases,tumor or other genetic diseases and with normal growth. Prothrombin time（PT）,prothrombin time international normalized ratio（PT-INR）,activated partial thromboplastin time（APTT）,fibrinogen（Fib） and thrombin time（TT） were determined. According to age,the 966 children were classified into perinatal newborn （0-7 d）,newborn（8-30 d）,infant 1（1-6 months）,infant 2（7-12 months）,child 1（1-2 years） and child 2（2-3 years） groups. The parameters were analyzed statistically,and the reference intervals were evaluated. Results PT showed no statistical significance between 1-6 months and 7-12 months and between 1-2 years and 2-3 years old groups（P>0.05）. PT were 12.20-15.08s for 0-7 d,11.84-14.20s for 8-30 d,11.05-13.45s for 1-12 months and 10.92-12.68s for 1-3 years. PT-INR were 1.07-1.45 for 0-7 d,0.99-1.27 for 8-30 d,0.82-1.10 for 1-12 months and 0.83-1.10 for 1-3 years. APTT showed no statistical significance between 0-7 d and 8-30 d,between 7-12 months and 1-2 years and between 7-12 months and 2-3 years old groups（P>0.05）. APTT were 21.27-58.20s for 0-30 d,24.67-51.52s for 1-6 months and 24.01-43.73s for 7 months-3 years. Fib showed no statistical significance between 8-30 d and 1-6 months,between 7-12 months and 1-2 years and between 7-12 months and 2-3 years old groups（P>0.05）. Fib were 1.43-3.97g/ L for 0-7 d,1.58-3.72g/L for 8 d-6 months and 2.65-3.89g/L for 7 months-3 years. TT showed no statistical significance between 0-7 d and 8-30 d,between 0-7 d and 1-6 months,between 7-12 months and 1-2 years and between 7-12 months and 2-3 years old groups（P>0.05）. TT were 15.31-24.83s for 0 d-6 months and 15.83-23.39s for 7 months-3 years. Conclusions Children's coagulation function is in a continuous growing process. There are physiological changes for each parameter of coagulation function. Laboratories should establish suitable reference intervals according to age,in order to provide a reference for clinical diagnosis and treatment of coagulation system diseases,preoperative examination and anticoagulant monitoring.

Key words: Coagulation function, Newborn, Infant, Reference interval

CLC Number:

R446.1

ZOU Chen, GAO Yuan. Establishment of the reference intervals of coagulation function in 0-3-year-old children[J]. Laboratory Medicine, 2018, 33(2): 106-109.

Figures/Tables 2

References 10

[1]	中华人民共和国卫生部. WS/T 359—2011 血浆凝固实验血液标本的采集及处理指南[S]. 2011.
[2]	陈武平. 438例新生儿凝血4项参考范围的探讨[J]. 中外健康文摘,2010,7(21):146-147.
[3]	MONAGLE P,BARNES C,IGNJATOVIC V,et al.Developmental haemostasis. Impact for clinical haemostasis laboratories[J]. Thromb Haemost,2006,95(2):362-372.
[4]	ANDREW M,PAES B,MILNER R,et a1. Development of the human coagulation system in the full-term infant[J]. Blood,1987,70(1):165-172.
[5]	李建新,徐安平,赖馨,等. 儿童年龄相关的常规凝血功能检测指标参考区间调查[J]. 中华检验医学杂志,2010,33(12):1193-1195.
[6]	吴娟,王正军,李治锋. 江苏省泗洪县内儿童凝血功能参考值范围建立[J]. 世界最新医学信息文摘(连续型电子期刊),2014,14(33):328-329.
[7]	毕玫荣,张岩. 新生儿凝血特点及其影响因素[J]. 国外医学·妇幼保健分册,2005,16(1):15217.
[8]	WILLIAMS M D,CHALMERS E A,GIBSON B E,et al.The investigation and management of neonatal haemostasis and thrombosis[J]. Br J Haematol,2002,119(2):295-309.
[9]	FLANDERS M M,CRIST B A,ROBERTS W L,et al.Pediatric reference intervals for seven common coagulation assays[J]. Clin Chem,2005,51(9):1738-1742.
[10]	叶应妩,王毓三,申子瑜. 全国临床检验操作规程[M]. 3版. 南京:东南大学出版社, 2006:96-101.

组别	PT（s）	PT-INR	APTT（s）	Fg（g/L）	TT（s）
围产期新生儿组（0~7 d）	13.64±1.44	1.26±0.19	39.74±18.47	2.70±1.27	20.08±4.77
新生儿组（8~30 d）	13.00±1.20^*	1.13±0.14^*	39.94±16.61	2.63±1.05^*	20.09±4.75
婴幼儿组1（1~6个月）	12.21±1.16^*	1.01±0.09^*	38.11±13.41^*	2.67±1.05	20.06±4.73
婴幼儿组2（7~12个月）	12.26±1.19	0.96±0.14	36.68±12.01^*	2.66±1.06^*	19.43±3.55^*
幼儿组1（1~2岁）	11.79±0.87^*	0.97±0.13^*	33.98±9.75	2.78±1.08	19.53±3.70
幼儿组2（2~3岁）	11.81±0.87	0.96±0.13	32.85±8.84	2.77±1.12	19.98±3.97

组别	PT（s）	PT-INR	APTT（s）	Fg（g/L）	TT（s）
围产期新生儿组（0~7 d）	13.64±1.44	1.26±0.19	39.74±18.47	2.70±1.27	20.08±4.77
新生儿组（8~30 d）	13.00±1.20^*	1.13±0.14^*	39.94±16.61	2.63±1.05^*	20.09±4.75
婴幼儿组1（1~6个月）	12.21±1.16^*	1.01±0.09^*	38.11±13.41^*	2.67±1.05	20.06±4.73
婴幼儿组2（7~12个月）	12.26±1.19	0.96±0.14	36.68±12.01^*	2.66±1.06^*	19.43±3.55^*
幼儿组1（1~2岁）	11.79±0.87^*	0.97±0.13^*	33.98±9.75	2.78±1.08	19.53±3.70
幼儿组2（2~3岁）	11.81±0.87	0.96±0.13	32.85±8.84	2.77±1.12	19.98±3.97

年龄	PT（s）	PT-INR
0~7 d	12.20~15.08	1.07~1.45
8~30 d	11.80~14.20	0.99~1.27
1~12个月	11.05~13.45	0.82~1.10
1~3岁	10.92~12.68	0.83~1.10

年龄	PT（s）	PT-INR
0~7 d	12.20~15.08	1.07~1.45
8~30 d	11.80~14.20	0.99~1.27
1~12个月	11.05~13.45	0.82~1.10
1~3岁	10.92~12.68	0.83~1.10

[1]	LI Xijiu, ZHOU Jianwei, ZHANG Qin, LONG Yunlin, WU Yingsong. Establishment and performance evaluation of UPLC-MS/MS for 6 markers in dried blood spots [J]. Laboratory Medicine, 2023, 38(5): 446-453.
[2]	AN Yinghong, YAO Kechun, SU Chengming, TANG Jinhua, YANG Lixin, GAO Jie, XU Yanting, YAN Qiuhong, ZHAN Hongyan, MA Yueyun. Survey of serum homocysteine levels of apparently healthy subjects in Beijing [J]. Laboratory Medicine, 2023, 38(11): 1036-1040.
[3]	ZHAO Yanhong, FU Lin, LIU Biao, XIAO Meifang. Reference interval of lactate dehydrogenase among 3-14-year-old population in Hainan [J]. Laboratory Medicine, 2023, 38(11): 1041-1043.
[4]	XU Qianqian, JIANG Wangqing, SHEN Linjie, LU Yingjie, CHEN Jian, JIANG Haoqin. Establishment of the reference intervals for related parameters of adult reticulocytes in Shanghai [J]. Laboratory Medicine, 2022, 37(7): 641-645.
[5]	ZHANG Zhibin, YANG Wanyong, RUAN Fuwang, WEN Qinghui. Roles of total IgE,sIgE and 25（OH）D in the evaluation of infant atopic dermatitis [J]. Laboratory Medicine, 2022, 37(3): 217-220.
[6]	MIAO Linzi, LU Yao, QU Chenxue, YOU Ran, GONG Yan. Establishment of the reference intervals of leukocyte surface CD64 and HLA-DR indexes for healthy adults and related factors [J]. Laboratory Medicine, 2022, 37(3): 240-245.
[7]	LU Youli, YANG Shuangshuang, ZHANG Meiwei, OU Meixian, DONG Chunxia, SHEN Weiwei, JIANG Fengli, LI Shuijun. Simultaneous determination of 5 steroid hormones in human serum by ultra-performance liquid chromatography-tandem mass spectrometry and establishment of reference intervals in healthy adults [J]. Laboratory Medicine, 2022, 37(2): 165-173.
[8]	ZHAO Xiaomin, JIANG Xiaoyu, SHAN Kaili, SHAN Tieying, LIU Xiuzhen. Roles of Flt-3L，TF and 25（OH）D in iron deficiency anemia among infants and children [J]. Laboratory Medicine, 2022, 37(11): 1039-1043.
[9]	YU Jianhong, PENG Qingyuan, YIN Minggang. Establishment of reference intervals of serum PGⅠ,PGⅡ and PGR for apparently healthy subjects in Zigong [J]. Laboratory Medicine, 2022, 37(1): 23-27.
[10]	LI Jing, QUAN Hui, ZENG Zhirong, WEI Yi. Establishment of the reference interval of small and dense low-density lipoprotein cholesterol in apparently healthy adults in Chengdu [J]. Laboratory Medicine, 2021, 36(6): 609-614.
[11]	WANG Zipeng, OUYANG Changli, GENG Deqin, WANG Dunjing. Establishment of cerebrospinal fluid IL-6 reference interval in non-inflammatory central nervous system diseases [J]. Laboratory Medicine, 2021, 36(6): 627-630.
[12]	YUE Bo, LI Danjie, TANG Dahai, LIU Manjiao, HE Jialei, JIANG Mengjie, WEI Xiaoqiang. Establishment of a new algorithm for the reference interval of relative change values and application evaluation [J]. Laboratory Medicine, 2021, 36(6): 667-673.
[13]	JI Zhi, DENG Lingyan, LI Jiaoyuan, WANG Xu, LI Huijun, CHENG Liming. Reference interval establishment of serum cytokeratin 19 fragment for healthy adults in Wuhan [J]. Laboratory Medicine, 2021, 36(5): 490-494.
[14]	XIE Hui, SHEN Han. Changes of coagulation function and influence factors of hypercoagulability in patients with chronic kidney disease [J]. Laboratory Medicine, 2021, 36(5): 500-503.
[15]	ZOU Ning, CHEN Guangxi, MA Hua, Qiurang, Zhaxinamo. Survey on the reference intervals of venous blood cells among native Tibetan adults in Aba county [J]. Laboratory Medicine, 2021, 36(2): 198-201.