Methods for determining 3 pathogenic secreted hydrolytic enzymes of oral Candida albicans and their application

doi:10.3969/j.issn.1673-8640.2016.05.003

Abstract

Abstract:

Objective To investigate in vitro expressions of 3 pathogenic secreted hydrolytic enzymes of oral Candida albicans. Methods The activities of secreted aspartyl proteinase（SAP）,phospholipase（PL） and lipase（Lip） from 88 isolates of oral Candida albicans were determined by bovine serum albumin agar,egg yolk agar and tributyrate agar,respectively. Results In the 88 isolates,70（79.5%） isolates showed high activity of SAP ,and 64（72.7%）isolates showed high activity of PL,while only 1（1.2% ） isolate showed high activity of Lip,and 67（76.1%） isolates produced medium activity of Lip. Positive correlation was found between SAP and PL（r = 0.221,P<0.05）,and no correlation was found between Lip and SAP or between Lip and PL（ r = 0.085 and 0.109,P = 0.432 and 0.311）. Conclusions Most of the isolates have high expressions of SAP and PL and medium expression of Lip. Secreted hydrolytic enzyme is one of the main virulence factors of oral Candida albicans.

Key words: Candida albicans, Secreted aspartyl proteinase, Phospholipase, Lipase

CLC Number:

R399.4

SUN Kangde, ZHANG Jiasheng, CHEN Xu, YU Zhongmin, CHEN Fuxiang. Methods for determining 3 pathogenic secreted hydrolytic enzymes of oral Candida albicans and their application[J]. Laboratory Medicine, 2016, 31(5): 355-358.

Figures/Tables 4

References 18

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标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.98	0.95	0.87	0.79	0.69	0.60	0.54	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	0.97	0.96	0.95	0.95	0.95

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.98	0.95	0.87	0.79	0.69	0.60	0.54	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	0.97	0.96	0.95	0.95	0.95

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.87	0.75	0.60	0.47	–	–	–	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.87	0.75	0.60	0.47	–	–	–	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00

标准菌株	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天
白念珠菌（ATCC 90028）	0.99	0.96	0.88	0.80	0.71	0.66	–	–
近平滑念珠菌（ATCC 22019）	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00