Decreased beta-lactam antibiotic susceptibility caused by outer membrane protein 38 deletion in Acinetobacter baumannii

doi:10.3969/j.issn.1673-8640.2016.03.017

Abstract

Abstract:

Objective To investigate the influence of outer membrane protein 38（Omp38） on the susceptibility of beta-lactam antibiotic in Acinetobacter baumannii. Methods The isolate with Omp38 deletion was induced by in vitro experiment. The contribution of mutation to beta-lactam antibiotic susceptibility was validated by complementation experiment. The cell membrane potential change of isolates was determined by flow cytometry. The growth rates were determined in order to evaluate mutant gene fitness cost. Results The Omp38 was confirmed to be correlated with the decreased beta-lactam antibiotic susceptibility. The Omp38 decreased cell membrane potential,and the fitness cost of Omp38 was approximately 3% through measuring growth rates（P< 0.05）. Conclusions Omp38 is deemed to play an important role in beta-lactam antibiotic resistance in Acinetobacter baumannii. The minor fitness cost of Omp38 may be a significant reason for the development of beta-lactam antibiotic resistance in Acinetobacter baumannii.

Key words: Outer membrane protein 38, Fitness cost, Membrane potential, Acinetobacter baumannii

CLC Number:

R446.5

WANG Dairong, LI Xi, ZHU Hong, WANG Aihua. Decreased beta-lactam antibiotic susceptibility caused by outer membrane protein 38 deletion in Acinetobacter baumannii[J]. Laboratory Medicine, 2016, 31(3): 224-227.

Figures/Tables 3

References 8

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菌株	头孢哌酮-舒巴坦	头孢曲松	氨曲南	头孢他啶	头孢噻肟	亚胺培南
ATCC 19606	1.5	48	24	4	24	0.38
19606-M24	32	64	48	6	24	0.19
M24-pwh 菌株	32	64	64	6	32	0.25
M24-p+Omp38 菌株	12	16	4	2	8	0.19

菌株	头孢哌酮-舒巴坦	头孢曲松	氨曲南	头孢他啶	头孢噻肟	亚胺培南
ATCC 19606	1.5	48	24	4	24	0.38
19606-M24	32	64	48	6	24	0.19
M24-pwh 菌株	32	64	64	6	32	0.25
M24-p+Omp38 菌株	12	16	4	2	8	0.19

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