Abstract:

Objective To observe the antibacterial efficiency of cefoperazone-sulbactam combined with traditional Chinese medicine against pan-drug resistant Acinetobacter baumannii（PDR-AB） in vitro and in vivo. Methods Different concentrations of cefoperazone-sulbactam and traditional Chinese medicine （gallnut,coptis,scutellaria,mint,fructus forsythia,fructus mume,schisandra chinensis and rheum officinale） were used combinedly. Through calculating minimal inhibitory concentration（MIC） and fractional inhibitory concentration（FIC）,the antibacterial efficiency in vitro of 36 isolates of PDR-AB was observed,and the appropriate method was chosen to evaluate the treatment for bacterial-infected mice by determining neutrophil,tumor necrosis factor-alpha （TNF-α）and procalcitonin（PCT）. Results Cefoperazone-sulbactam combined with gallnut,coptis and scutellaria had synergized effect,while combined with schisandra chinensis,mint and fructus forsythia, it had additive effect. When combined with fructus mume and rheum officinale, it showed antagonised effect,and it combined with gallnut could significantly reduce the levels of neutrophil,TNF-α and PCT. Conclusions Cefoperazone-sulbactam combined with gallnut has antibacterial efficiency for PDR-AB.

Key words: Pan-drug resistant Acinetobacter baumannii, Cefoperazone-sulbactam, Traditional Chinese medicine