Laboratory Medicine ›› 2015, Vol. 30 ›› Issue (6): 626-630.DOI: 10.3969/j.issn.1673-8640.2015.06.020

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Study on carbapenemase and integrase genotypes of carbapenem-resistant Acinetobacter baumannii

BU Jingsong, WANG Yong   

  1. Department of Clinical Laboratory, Shangyu People's Hospital, Zhejiang Shaoxing 312300, China
  • Received:2014-12-19 Online:2015-06-30 Published:2015-07-03

Abstract: Objective

To investigate the genotypes of carbapenemase and integrase by carbapenem-resistant Acinetobacter baumannii (CRAB).

Methods

A total of 93 isolates of CRAB and 16 isolates of carbapenem-sensitive Acinetobacter baumannii (CSAB) were collected. Carbapenemases were detected by modified Hodge testing. Metallo-beta-lactamase was detected by EDTA collaborative testing. Carbapenemase and integrase genotypes were amplified by polymerase chain reaction(PCR)and sequencing.

Results

The carbapenemase phenotype test results showed that 62.4% isolates were positive, and metal enzymes were all negative. The PCR results for CRAB showed the detection rates of OXA-23 and Int1 were 100%, for OXA-51 was 96.8%, for OXA-58 was 1.1%, and OXA-24, VIM, IMP and Int2 were not found. The PCR results for CSAB showed the detection rate of Int1 was 37.5%.

Conclusions

CRAB mainly carrys OXA-23 and OXA-51 carbapenemase genes, and all isolates carry ClassⅠ integrase gene.

Key words: Acinetobacter baumannii, Carbapenemase, Integrase, Genotype

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