Abstract:

Objective To investigate the polymorphism distribution of aspirin efficacy related platelet membrane glycoprotein GPⅠa gene G873A and C807T in cardio-cerebrovascular disease and diabetes mellitus. Methods A total of 811 patients from September 2018 to October 2020 in Tongling People's Hospital,including 106 cases of cardiovascular diseases,597 cases of cerebrovascular diseases,108 cases of diabetes mellitus,were enrolled,and the polymorphisms of GPⅠa G873A and C807T were determined by pyrophosphate sequencing method. Results Wild type,heterozygous mutation type and homozygous mutation type of GPⅠa G873A and C807T all synchronously appeared in the same cases,the sum of heterozygous mutation type and homozygous mutation type was 47.17%-53.27%,and the sum of allele type A and type T was 24.53%-31.48%. In diabetes mellitus group,homozygous mutation type of 2 gene loci accounted for 13.89%,which was higher than those in cardiovascular disease group（1.89%） and cerebrovascular disease group（8.21%）,while the percentage of heterozygous mutation type（35.19%） was lower than those in cardiovascular disease group（45.28%） and cerebrovascular disease group（45.06%）,and compared with cardiovascular disease group and cerebrovascular disease group,there was statistical significance for genotype distribution（P<0.01）. There was no statistical significance for allele distributions among the 3 groups（P>0.05）. There was no statistical significance for 2 genetic locus genotypes and allele distributions between males and females among the 3 groups（P>0.05）. The cardiovascular and cerebrovascular disease patients by age were classified into 29-60-year-old,61-70-year-old,71-80-year-old and 81-94-year-old groups,respectively,and 2 genetic locus genotype and allele distributions had no statistical significance（P>0.05）. Conclusions Polymorphism distribution of aspirin efficacy related GPⅠa gene G873A and C807T has no correlation with sex and age,and the mutation phenomenon of the 2 gene loci is relatively common. The mutations exist differences between individuals,which might affect the clinical effect of anti-platelet therapy. The rate of homozygous mutation of the 2 gene loci in diabetes mellitus group is higher than those in cardiovascular and cerebrovascular disease groups.

Key words: GPⅠa gene G873A, C807T, Aspirin, Efficacy, Genotype