Abstract: To evaluate B-type natriuretic peptide quantification by single-epitope sandwich(SES)combined with cyclic enhanced immunofluorescent assay(CEIFA). By SES combined with CEIFA, BNP was determined. The precision, linear range, function sensitivity, recovery rate and sample stability were evaluated, and 45 samples were determined by the method, which the results were evaluated with those of SIEMENS ADVIA Centaur CP chemiluminescence analyzer (Centaur CP). The within-run and between-run coefficients of variation(CV) were 4.38%-7.84% and 10.47%-11.21%, respectively. The linear range was 25-5 000 pg/mL. The function sensitivity was 12.5 pg/mL. The recovery rate was 95.04%-109.2%. The correlation formula between the novel method and Centaur CP was Y=0.998 3X-22.38 (r=0.969, P<0.01). The request of sample stability of SES combined with CEIFA was lower than that of Centaur CP. SES combined with CEIFA for BNP quantification has a good performance, and there are some advantages compared with traditional double-antibody sandwich chemiluminescence assay.

Key words: B-type natriuretic peptide, Single-epitope sandwich, Immunofluorescent assay, Heart failure