The clinical application of PCT in the diagnosis of systemic and local bacterial infections

doi:10.3969/j.issn.1673-8640.2015.01.005

Abstract

Abstract:

Objective To evaluate the clinical application significance of serum procalcitonin(PCT)quantitation determination in the diagnosis of systemic and local bacterial infections. Methods The Clinical and Laboratory Standards Institute (CLSI) EP15-A2 document was employed to verify the precision and accuracy of PCT determination by Roche Cobas E601 chemiluminescence analyzer (Cobas E601) and ensure instrument conditions being complied with the requirement of experiment. A total of 697 patients with systemic and local bacterial infections were enrolled. The results of serum PCT determination, blood culture, sputum culture, middle-urine culture and cerebrospinal culture were used to compare the change trends of serum PCT for systemic and local bacterial infections and for different-part infections. The difference of PCT in Gram-positive cocci and Gram-negative bacilli infections was analyzed. Results Cobas E601 had good repeatability, and the total coefficient of variation (CV) was <3.59%. The fixed calibration bias was <5.0% and met the clinical determination requirements. For serum PCT, there was significance for systemic and local bacterial infections with having increasing serum PCT level(P<0.05). For local bacterial infection, there was no statistical significance for respiratory system, urinary system and nervous system infections (P>0.05). For systemic bacterial infection, there was higher serum PCT level for patients with Gram-negative bacilli infection than with Gram-positive cocci infection (P<0.05). For local bacterial infection, there was no statistical significance for patients with Gram-negative bacilli infection and Gram-positive cocci infection (P>0.05). Conclusions In patients with systemic and local bacterial infections, serum PCT level has high expression, and serum PCT level can not identify local bacterial infection Gram-negative bacilli and Gram-positive cocci infections.

Key words: Procalcitonin, Systemic bacterial infection, Local bacterial infection, Gram-negative bacillus, Gram-positive coccus

CLC Number:

R446.5

MU Yuejing, WANG Weijia, YAN Haizhong, XU Quanzhong, KAN Lijuan, OUYANG Nengliang, HUANG Yanhua, ZHANG Xiuming.. The clinical application of PCT in the diagnosis of systemic and local bacterial infections[J]. Laboratory Medicine, 2015, 30(1): 17-20.

Figures/Tables 1

References 13

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项目	均值(ng/mL)	批内标准差(ng/mL)	批内CV(%)	总标准差(ng/mL)	总CV(%)
PC1	0.55	0.01	2.61	0.03	2.94
PC2	10.46	1.24	1.44	1.35	1.55
PC3	0.10	0.01	3.57	0.01	3.59
PC4	0.48	0.01	2.87	0.02	3.09
PC5	56.84	3.49	1.33	4.19	1.93

项目	均值(ng/mL)	批内标准差(ng/mL)	批内CV(%)	总标准差(ng/mL)	总CV(%)
PC1	0.55	0.01	2.61	0.03	2.94
PC2	10.46	1.24	1.44	1.35	1.55
PC3	0.10	0.01	3.57	0.01	3.59
PC4	0.48	0.01	2.87	0.02	3.09
PC5	56.84	3.49	1.33	4.19	1.93

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