The preliminary evaluation of multiple genetic analysis system to detect Helicobacter pylori

doi:10.3969/j.issn.1673-8640.2014.04.013

Abstract

Abstract:

Objective　To evaluate a new kind of Helicobacter pylori（HP） detection method， using the multiple genetic analysis system（GeXP system） to detect HP infection. Methods　Use culture method， rapid urease test， ordinary polymerase chain reaction（PCR） and multiple genetic testing to determine whether gastric biopsies exist HP. The sensitivity， specificity， positive predictive value and negative predictive value were evaluated by Stata 12.0 statistical software， in order to evaluate the testing performance of GeXP system from horizontal degree. Furthermore， multiple genetic testing was performed by selecting 5 specific genes（16S rRNA, ureA, ureC, 26KDa and cagA genes）as HP diagnosis infection， and multiple genetic testing was evaluated from longitudinal degree. Results　The sensitivity of culture method was 76.8%， the specificity was 100.0%， the positive predictive value was 100.0%， and the negative predictive value was 57.7%. For rapid urease test， by contrast， its sensitivity was 87.8%， the specificity was 80.8%， the positive predictive value was 93.5%， and the negative predictive value was 67.7%. Ordinary PCR had the highest sensitivity and specificity（100.0%）. For GeXP system， the sensitivity was 100.0%， the specificity was 71.3% without false negative case， the positive predictive value was 90.9%， and the negative predictive value was 100.0%. Conclusions　The new preliminary multiple genetic testing， GeXP system， has high sensitivity and specificity and high flux characteristics， which would not only meet the needs of clinical sample determination， but also could monitor prognosis after receiving eradication therapy. The GeXP system has a promising application significance.

Key words: Multiple genetic analysis, GeXP system, Helicobacter pylori, Identification, Gene, High flux

CLC Number:

R446.5

ZHOU Lifang, BAO Zhijun,MIAO Yingxin,JI Danian,HUANG Yiqin,ZHAO Fuju,ZHAO Hu.. The preliminary evaluation of multiple genetic analysis system to detect Helicobacter pylori[J]. , 2014, 29(4): 350-356.

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