Abstract:

Objective　To investigate the clinical application significance of high risk-type human papillomavirus（HPV） DNA detection to screen cervix precancerous lesion in healthy examination. Methods　A total of 2 827 married females for healthy examination did the common gynecological examination. Their samples were detected for thinprep cytology test（TCT） and HPV 13 high risk-type DNA. Results　The infection rate of high risk-type HPV was 15.9%， and the infection rates of high risk-type HPV in 3 different groups（＜30 years old， 30-45 years old and ＞45 years old） had no statistical significance（P＞0.05）. The 83.4％ females who had high risk-type HPV infection could remove virus infection in 1 year， and 372 females who had no high risk-type HPV infection did not have precancerous lesion or cervical cancer at least 2 years. The infection rate of high risk-type HPV in 2 groups of cervix slick or low-degree erosion and cervical erosionⅡor above degree had statistical significance（P＜0.05）. The screen rate of cervix precancerous lesion and cervical cancer using TCT and high risk-type HPV DNA combination detection was 72.7%. Conclusions　The application of high risk-type HPV DNA detection in healthy examination has early diagnosis， prevention and treatment significance in the negative prediction of high risk-type HPV infection and persistent infection of HPV above 12 months. TCT and high risk-type HPV DNA combination detection can screen cervical cancer and cervix precancerous lesion efficiently.

Key words: High risk-type human papillomavirus, Cervical cancer, Healthy examination