Abstract:

Objective To study the infection characteristics of human papillomavirus（HPV）in cervical cells from different lesion tissues,and to investigate the relationship between HPV infection and the severity of cervical lesions. Methods A total of 53 cases of normal or mild inflammation,79 cases of cervical intraepithelial neoplasia（CIN）Ⅰ,42 cases of CIN Ⅱ,87 cases of CIN Ⅲ,or 54 cases of invasive cervical cancer（ICC）were determined for 23 HPV genotypes by multiplex polymerase chain reaction（PCR）-reverse dot blotting（RDB）. The infection characteristics of HPV and the severity of cervical lesions were evaluated,and the relationship between different HPV genotypes and the severity of cervical lesions were investigated as well. Results HPV infection and the severity of cervical lesions had correlation（r =0.500）,and the determination rate of HPV increased as the severity of cervical lesions increased（χ²=43.322,P<0.05）. The determination rates of HPV with different grades of cervical lesions had statistical significance（P<0.01）,except CIN Ⅲ and ICC groups（χ²=0.003,P>0.05）. Multiple HPV infections were related with the severity of cervical lesions（r =0.496,P<0.05）. The determination rate of multiple HPV increased as the severity of cervical lesions increased,except ICC group（P<0.01）. The determination rate and the proportion of high-risk HPV infection were increased with the severity of cervical lesions. Different HPV genotypes had statistical significance on cervical lesions（χ²=46.775,P<0.05）. The top 5 HPV genotypes to cervical lesions were type 16,18, 31,33 and 58. Conclusions The severity of cervical lesions is correlated with HPVgenotypes and multiple HPV infections. The proportions of HPV genotypes among different cervical lesion grades are different. With the severity of cervical lesions increased,the determination rate of high-risk HPV infection and its proportion are increased.

Key words: Human papillomavirus, Cervical intraepithelial neoplasia, Cervical cancer, Genotype