Mixed infection status of human papillomavirus,Ureaplasma urealyticum,Chlamydia trachomatis and Neisseria gonorrhoeae in Changning district of Shanghai

doi:10.3969/j.issn.1673-8640.2020.09.001

Abstract

Abstract:

Objective To investigate the mixed infection status and correlations of human papillomavirus（HPV） with Ureaplasma urealyticum（UU）,Chlamydia trachomatis（CT） and Neisseria gonorrhoeae（NG） among female patients in Changning district of Shanghai. Methods Totally,4 496 samples,including cervical exudate cell and cervical swab samples,of female patients from Shanghai Jiao Tong University School of Medicine Tongren Hospital were collected from June 1st 2018 to May 31st 2019. A total of 113 healthy subjects were enrolled as healthy control group. CT DNA,UU DNA and NG DNA were determined by fluorescence probe-polymerase chain reaction（PCR）,and PCR-reverse dot blot was used to determine HPV DNA subtypes. The positive rate and subtype distribution characteristics were analyzed and compared with those of healthy control group. The determination rates of CT DNA,UU DNA and NG DNA were investigated by classification according to the negativity or positivity and infection types of HPV. Results The determination rate of UU DNA was the highest（65.9%）,followed by HPV DNA（23.9%）,and the determination rate of NG DNA was the lowest（0.9%）. Compared with healthy control group,the determination rates of UU DNA,CT DNA and NG DNA were 62.8%（71/113）,3.6%（4/113）and 0.9%（1/113）,and the determination rate of HPV was 22.1%. The subtype with the highest determination rate of HPV DNA was high-risk HPV52. Compared with the other types,the determination rates of CT,NG and UU were also high（23.2%,2.9% and 20.9%,respectively）. The determination rates of UU,CT and NG in HPV positive group were higher than those in HPV negative group（P<0.001）. With the increasing number of HPV DNA subtypes,the positive rates of CT,NG and UU were increased. The positive rates of CT and UU in patients with 4 or more subtypes of HPV infections（20.00% and 87.7%） were higher than those in patients with single subtype of HPV infection（10.8% and 76.9%）（P<0.05）. Conclusions The determination rate of UU is higher than those of HPV,NG and CT. With the occurrence of multiple infection of different subtypes of HPV,the infection rates of CT,UU and NG increase,and mixed infection occurs. It should pay attention to the occurrence of multiple infection of HPV.

Key words: Human papillomavirus, Chlamydia trachomatis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Mixed infection

CLC Number:

R373.9

XUAN Binbin, TAN Meiyu, SUN Hanxiao, SHENG Huiming. Mixed infection status of human papillomavirus,Ureaplasma urealyticum,Chlamydia trachomatis and Neisseria gonorrhoeae in Changning district of Shanghai[J]. Laboratory Medicine, 2020, 35(9): 859-863.

Figures/Tables 3

References 13

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HPV亚型	HPV（1 073例）		HPV合并UU（832例）		HPV合并CT（138例）		HPV合并NG（22例）
HPV亚型	例数	百分比/%	例数	百分比/%	例数	百分比/%	例数	百分比/%
52	233	21.7	174	20.9	32	23.2	4	2.9
16	161	15.0	123	14.8	25	18.1	3	2.2
53	127	11.8	104	12.5	17	12.3	4	2.9
58	127	11.8	99	11.9	16	11.6	4	2.9
81（低危型）	121	11.3	100	12.0	18	13.0	1	0.7
51	113	10.5	91	10.9	15	10.9	3	2.2
43（低危型）	99	9.2	80	9.6	20	14.5	0	0.0
18	86	8.0	67	8.1	17	12.3	1	0.7
42（低危型）	86	8.0	68	8.2	11	8.0	1	0.7
68	83	7.7	67	8.1	13	9.4	4	2.9
59	76	7.1	61	7.3	20	14.5	3	2.2
56	56	5.2	47	5.6	6	4.3	1	0.7
6（低危型）	51	4.8	40	4.8	9	6.5	2	1.4
66	50	4.7	43	5.2	2	1.4	0	0.0
39	45	4.2	37	4.4	5	3.6	1	0.7
33	43	4.0	27	3.2	9	6.5	1	0.7
35	37	3.5	24	2.9	1	0.7	0	0.0
11（低危型）	36	3.4	31	3.7	7	5.1	2	1.4
31	34	3.2	26	3.1	4	2.9	1	0.7
73	25	2.3	15	1.8	6	4.3	0	0.0
45	24	2.2	22	2.6	1	0.7	1	0.7
82	14	1.3	13	1.6	1	0.7	1	0.7
83（低危型）	8	0.8	8	1.0	1	0.7	0	0.0

HPV亚型	HPV（1 073例）		HPV合并UU（832例）		HPV合并CT（138例）		HPV合并NG（22例）
HPV亚型	例数	百分比/%	例数	百分比/%	例数	百分比/%	例数	百分比/%
52	233	21.7	174	20.9	32	23.2	4	2.9
16	161	15.0	123	14.8	25	18.1	3	2.2
53	127	11.8	104	12.5	17	12.3	4	2.9
58	127	11.8	99	11.9	16	11.6	4	2.9
81（低危型）	121	11.3	100	12.0	18	13.0	1	0.7
51	113	10.5	91	10.9	15	10.9	3	2.2
43（低危型）	99	9.2	80	9.6	20	14.5	0	0.0
18	86	8.0	67	8.1	17	12.3	1	0.7
42（低危型）	86	8.0	68	8.2	11	8.0	1	0.7
68	83	7.7	67	8.1	13	9.4	4	2.9
59	76	7.1	61	7.3	20	14.5	3	2.2
56	56	5.2	47	5.6	6	4.3	1	0.7
6（低危型）	51	4.8	40	4.8	9	6.5	2	1.4
66	50	4.7	43	5.2	2	1.4	0	0.0
39	45	4.2	37	4.4	5	3.6	1	0.7
33	43	4.0	27	3.2	9	6.5	1	0.7
35	37	3.5	24	2.9	1	0.7	0	0.0
11（低危型）	36	3.4	31	3.7	7	5.1	2	1.4
31	34	3.2	26	3.1	4	2.9	1	0.7
73	25	2.3	15	1.8	6	4.3	0	0.0
45	24	2.2	22	2.6	1	0.7	1	0.7
82	14	1.3	13	1.6	1	0.7	1	0.7
83（低危型）	8	0.8	8	1.0	1	0.7	0	0.0

组别	CT		NG		UU		CT/NG/UU
组别	阳性例数	阳性率/%	阳性例数	阳性率/%	阳性例数	阳性率/%	阳性例数	阳性率/%
HPV阳性组	138	12.9^*#	22	2.1^*#	832	77.5^*#	857	79.9^*#
HPV阴性组	172	5.0	20	0.6	2 133	62.3	2 185	63.8
正常对照组	71	3.5	1	0.9	71	62.8	71	62.8

组别	CT		NG		UU		CT/NG/UU
组别	阳性例数	阳性率/%	阳性例数	阳性率/%	阳性例数	阳性率/%	阳性例数	阳性率/%
HPV阳性组	138	12.9^*#	22	2.1^*#	832	77.5^*#	857	79.9^*#
HPV阴性组	172	5.0	20	0.6	2 133	62.3	2 185	63.8
正常对照组	71	3.5	1	0.9	71	62.8	71	62.8

组别	CT		NG		UU		CT/NG/UU
组别	阳性例数	阳性率/%	阳性例数	阳性率/%	阳性例数	阳性率/%	阳性例数	阳性率/%
单一感染	73	10.8	11	1.6	518	76.9	530	78.6
双重感染	39	15.1	6	2.3	199	77.1	209	81.0
三重感染	13	17.1	5	6.6^*	58	76.3	60	78.9
四重及以上感染	13	20.0^*	0	0	57	87.7^*	57	87.7