The induction of vascular endothelial microparticles to T cell activation and the cytokine production of Th1 type

doi:10.3969/j.issn.1673-8640.2014.04.014

Abstract

Abstract:

Objective　To study the influence of vascular endothelial microparticles（EMP） on T lymphocyte activation in vitro and the cytokine production， and to study the roles of EMP on immune response. Methods　Lymphocytes from BN rats were interfered by EMP derived from human endothelial cells and SD rat′s endothelial cells. The early activation CD69 expression of T lymphocytes（CD3⁺ lymphocytes） was measured by flow cytometry. Cytokines such as interferon-gamma（IFN-γ） and interleukin 2（IL-2） were measured for expression by enzyme-linked immunosorbent assay（ELISA）. Results　Compared with those of control group [adding same volune of phosphate buffer（PBS）]， the expressions of CD69 and IFN-γ in culture supernatant of each EMP concentration group increased remarkably（P＜0.05）. Compared with those of control group， the expression of IL-2 increased remarkably in culture supernatant of intermediate and high EMP concentration groups with statistical significance（P＜0.05）. Conclusions　EMP can induce T lymphocyte activation and Th1 cytokine production. EMP may play an important role in immune response through activating T lymphocytes.

Key words: Endothelial microparticle, Activation, CD69, Interferon-gamma, Interleukin 2, T lymphocyte

CLC Number:

R446.63

LIU Changying，SUN Yi. The induction of vascular endothelial microparticles to T cell activation and the cytokine production of Th1 type[J]. , 2014, 29(4): 357-365.

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