Abstract: Objective To study the correlation between T lymphocyte subgroup，liver function indexes and traditional Chinese medicine（TCM） syndrome differentiation in chronic hepatitis B（CHB） patients，and to provide a reference for clinical TCM syndrome differentiation，choice of treatment and observation of therapeutic efficacy. Methods Totally，983 patients with CHB were enrolled，and the types of TCM syndrome differentiation were as follows：solid syndrome（270 cases，including 75 cases of damp-heat obstruction syndrome and 195 cases of blood stasis obstruction syndrome） and deficiency syndrome（713 cases，including 662 cases of liver-depression and spleen-deficiency syndrome，28 cases of liver-kidney yin-deficiency syndrome and 23 cases of spleen-kidney yang-deficiency syndrome）. By determining liver function indexes [alanine aminotransferase（ALT），aspartate aminotransferase（AST），total protein（TP），albumin（Alb），total bilirubin（TB），direct bilirubin（DBIL）] and T lymphocyte counts（CD3⁺，CD4⁺，CD8⁺），the correlation between each index and TCM syndrome was evaluated. Results The frequency of CHB was as follows：liver-depression and spleen-deficiency>blood stasis obstruction>damp-heat obstruction>liver-kidney yin-deficiency>spleen-kidney yang-deficiency. Among the CHB syndromes，the patients with spleen-kidney yang-deficiency syndrome were elder，and the patients with damp-heat obstruction syndrome were young. Liver function indexes and T lymphocyte counts had statistical significance among the different syndromes. CD3⁺ and CD4⁺ cell counts were positively correlated with solid and damp-heat obstruction syndromes（P<0.05），and CD3⁺ and CD4⁺ T cell counts were negatively correlated with spleen-kidney yang-deficiency syndrome（P<0.05）. Conclusions The liver function indexes and T lymphocyte subgroup are correlated with TCM syndrome differentiation in CHB patients，which can provide a reference for clinical TCM syndrome differentiation，choice of treatment and observation of therapeutic efficacy.

Key words: T lymphocyte subgroup, Liver function, Chronic hepatitis B, Traditional Chinese medicine syndrome differentiation