关键词: 呼吸道感染, 非典型性病原体, 间接荧光免疫法, 多重感染, 阳性率

Abstract:

Objective To investigate the roles of the detections of 8 pathogen IgM antibodies in patients with different pathogens of respiratory tract infection. Methods From June 2018 to June 2019,3 600 patients with respiratory tract infection were enrolled from Jiaxing Hospital of Traditional Chinese Medicine of Zhejiang Chinese Medical University. Indirect fluorescence immunoassay was used to detect 8 pathogen IgM antibodies,including adenovirus（ADV）,Legionella pneumophila（LP）,Chlamydia pneumoniae（CP）,respiratory syncytial virus（RSV）,influenza A virus（IFA）,Mycoplasma pneumoniae（MP）,influenza B virus（IFB） and parainfluenza virus（PIVS） 1,2 and 3. Results A total of 522 cases of pathogen IgM antibodies were detected in 3 600 patients with respiratory tract infection,with a total positive rate of 14.50%. IFB-IgM,IFA-IgM and MP-IgM accounted for 6.11%,4.36% and 2.17%,respectively. There was a statistical significance for the positive rates of IgM antibody detection in MP,IFA,IFB and PIVS in different seasons（P<0.05）. The infection rate of MP in autumn and winter was higher than that in spring and summer,and the infection rates of IFA and IFB in winter and spring were higher than those in summer and autumn. The infection rate of PIVS in spring and summer was higher than that in autumn and winter（P<0.05）. The infection rates of LP,CP,ADV and RSV in different seasons had no statistical significance（P>0.05）. The positive rate of single infection was the highest among all the patients with respiratory tract infection,and the positive rate of infection decreased gradually with the increase of the number of pathogens（P<0.05）. There was no statistical significance in the probability of infection of different pathogens among all the groups（P>0.05）. Conclusions The detection of IgM antibodies of 8 pathogens is of significance for the early diagnosis of respiratory tract infection,and it has guiding significance for the prevention of multiple infections in multiple infection season.

Key words: Respiratory tract infection, Atypical pathogen, Indirect fluorescence immunoassay, Multiple infection, Positive rate