检验医学 ›› 2017, Vol. 32 ›› Issue (9): 818-821.DOI: 10.3969/j.issn.1673-8640.2017.09.016

• 技术研究与评价·论著 • 上一篇    下一篇

尿液常规分析干化学法24 h内稳定性评价

王刚强, 程翔, 郑善銮   

  1. 第四军医大学第一附属医院全军临床检验医学中心,陕西 西安 710032
  • 收稿日期:2016-06-02 出版日期:2017-09-30 发布日期:2017-09-30
  • 作者简介:null

    作者简介:王刚强,男,1987年生,学士,主管技师,主要从事临床检验工作。

Stability of routine urine dry chemical method in 24 h

WANG Gangqiang, CHENG Xiang, ZHENG Shanluan   

  1. People's Liberation Army Center for Clinical Laboratory Medicine,the First Affiliated Hospital of the Fourth Military Medical University,Xi'an 710032,Shaanxi,China
  • Received:2016-06-02 Online:2017-09-30 Published:2017-09-30

摘要: 目的

探讨尿液常规分析干化学法24 h内重复检测结果的稳定性。

方法

选取318例患者新鲜尿液标本,在收集到标本后立刻上机检测,所得结果即为0 h结果,然后分别在常温(25±2)℃和4 ℃下,2、4、6、24 h内检测尿液干化学10项:酸碱度(pH值)、比重(SG)、白细胞(LEU)、隐血(ERY)、亚硝酸盐(NIT)、蛋白质(PRO)、葡萄糖(Glu)、胆红素(Bil)、尿胆原(URO)、酮体(KET)。

结果

与0 h时相比,常温(25±2)℃下4 h LEU、ERY的变化差异有统计学意义(P<0.05),4 ℃下6 h LEU、ERY的变化差异有统计学意义(P<0.05);其他项目结果无论是常温还是4 ℃冷藏在24 h内变化差异均无统计学意义(P>0.05)。

结论

尿液常规分析干化学法不同项目在24 h内的稳定性不同,常温保存应于2 h内完成检测,4 ℃运送保存应在4 h内完成检测。对于一些特殊疾病的患者标本应尽可能优先处理,从而保证其结果稳定、准确。

关键词: 尿液, 放置时间, 稳定性

Abstract:

Objective To investigate the stability of routine urine dry chemical method in 24 h. Methods A total of 318 fresh urine specimens were collected. The specimens were determined immediately after collection,and the results were named as the results of 0 h. The specimens were determined after collection for 2,4,6 and 24 h,under room temperature(25± 2)℃ and 4 ℃,respectively. The pH value,specific gravity(SG),leukocyte(LEU),erythrocyte(ERY),nitrite(NIT),protein(PRO),glucose(Glu),bilirubin(Bil),urobilinogen(URO) and ketone body(KET) were determined. Results Compared with the results of 0 h,the results of LEU and ERY of 4 h at room temperature had statistical significance(P<0.05). The changes of LEU and ERY of 6 h at 4 ℃ had statistical significance(P<0.05),but there was no statistical significance for other parameters within 24 h at either room temperature or 4 ℃(P>0.05). Conclusions The stabilities of urine dry chemical method for different parameters within 24 h are different. The urine specimens should be determined within 2 h at room temperature,or within 4 h at 4 ℃. Some urine specimens from patients with special diseases should be determined as soon as possible,to ensure stability and accuracy.

Key words: Urine, Storage period, Stability

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