子痫前期孕妇孕早期血清miR-520g表达分析

doi:10.3969/j.issn.1673-8640.2015.04.012

检验医学 ›› 2015, Vol. 30 ›› Issue (4): 345-348.DOI: 10.3969/j.issn.1673-8640.2015.04.012

子痫前期孕妇孕早期血清miR-520g表达分析

厉倩, 龙安雄, 洪茂, 蔡雷鸣, 谢骊, 顾继安, 谭龙益

上海市第一人民医院宝山分院检验科,上海 200940

收稿日期:2014-04-27 出版日期:2015-04-30 发布日期:2015-05-16
作者简介:null
作者简介:厉倩,女,1980年生,硕士,主管技师,主要从事分子生物学检验工作。
基金资助:
上海市卫生局青年科研项目 (20124Y080)

The analysis of serum miR-520g expression in the early stage of preeclampsia among pregnant patients

LI Qian, LONG Anxiong, HONG Mao, CAI Leiming, XIE Li, GU Ji'an, TAN Longyi.

Department of Clinical Laboratory, Baoshan Branch, Shanghai First People's Hospital, Shanghai 200940, China

Received:2014-04-27 Online:2015-04-30 Published:2015-05-16

摘要/Abstract

摘要：

目的探讨血清微小RNA(miR)-520g在子痫前期(PE)患者孕期的变化及意义。方法分别提取30例PE患者和30名正常妊娠者早、中、晚孕期血清中的循环miR,利用实时荧光定量逆转录聚合酶链反应(PCR)检测血清中的miR-520g的表达量,比较两组间不同孕期miR-520g的差异。结果正常妊娠组随孕程进展血清miR-520g的表达量增加;与正常妊娠组相比,PE组孕早期miR-520g显著增加,PE组孕中、晚期的血清miR-520g表达量与正常妊娠组差异无统计学意义。结论孕早期血清miR-520g可能是PE预测的良好标志物。

关键词: 子痫前期, 微小RNA, 血清, 生物标志物

Abstract:

Objective To investigate the change and significance of serum microRNA (miR)-520g in preeclampsia among pregnant patients. Methods Serum cycling miR was extracted from 30 patients with preeclampsia and 30 healthy pregnant women at early, middle and late pregnant stages. The expression of miR-520g was determined by quantitive reverse transcription polymerase chain reaction(PCR), and the difference between the 2 groups was analyzed statistically. Results MiR-520g increased with pregnant days in healthy pregnant group. MiR-520g in early pregnant stage of preeclampsia group increased comparing with that of healthy pregnant group, whereas no significant difference was observed in middle and late pregnant stages between preeclampsia group and healthy pregnant group. Conclusions Serum miR-520g in early pregnant stage may be a potential biomarker for the prediction of preeclampsia.

Key words: Preeclampsia, MicroRNA, Serum, Biomarker

中图分类号:

Q503

厉倩, 龙安雄, 洪茂, 蔡雷鸣, 谢骊, 顾继安, 谭龙益. 子痫前期孕妇孕早期血清miR-520g表达分析[J]. 检验医学, 2015, 30(4): 345-348.

LI Qian, LONG Anxiong, HONG Mao, CAI Leiming, XIE Li, GU Ji'an, TAN Longyi.. The analysis of serum miR-520g expression in the early stage of preeclampsia among pregnant patients[J]. Laboratory Medicine, 2015, 30(4): 345-348.

图/表 3

表1 研究对象临床特征

组别	例数	年龄 (岁)	孕前BMI (kg/m²)	PE诊断孕周
PE组	30	30(23~37)	22.6±3.1	35.3±2.7
正常妊娠组	30	28(22~35)	21.3±2.6	-

图1 正常妊娠组不同孕周血清miR-520g表达量注:与正常孕12~14周比较,*P<0.05

图2 PE患者与正常妊娠者不同孕周血清miR-520g相对表达量的比较注: 正常妊娠组;PE组;与正常妊娠组孕12~14周比较,*P<0.05

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子痫前期孕妇孕早期血清miR-520g表达分析

The analysis of serum miR-520g expression in the early stage of preeclampsia among pregnant patients

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