关键词: 染色体, 核型, Y染色体微缺失, 无精症, 严重少精症

Abstract:

Objective 　To investigate chromosome karyotypes and Y chromosome microdeletions and their distribution characteristics in patients with azoospermia or severe oligozoospermia， in order to provide thev reference for genetic screening of intracytoplasmic sperm injection（ICSI） before pregnancy. Methods　A total of 150 patients with azoospermia or severe oligozoospermia were enrolled. Chromosome karyotype analysis was performed on peripheral blood lymphocytes with G-banding. Y chromosome microdeletions were detected by multiplex polymerase chain reaction（PCR） combined with agarose gel electrophoresis. Results　There were 14 cases of abnormal chromosome karyotypes and 16 cases of Y chromosome microdeletions in 150 patients with azoospermia or severe oligozoospermia， and the abnormal rates were 9.3% and 10.7%， respectively. Conclusions　For patients with azoospermia or severe oligozoospermia， the chromosome karyotype analysis and Y chromosome microdeletion determinations could provide genetic screening reference before performing ICSI treatments， avoiding the genetic deficiency being passed to offspring and reducing the patients' mental and economic burdens.

Key words: Chromosome, Karyotype, Y chromosome microdeletion, Azoospermia, Severe oligozoospermia