新生儿脐血IgE与AICDA基因多态性的相关性

doi:10.3969/j.issn.1673-8640.2014.02.016

检验医学 ›› 2014, Vol. 29 ›› Issue (2): 158-161.DOI: 10.3969/j.issn.1673-8640.2014.02.016

• 技术研究与评价·论著 • 上一篇下一篇

新生儿脐血IgE与AICDA基因多态性的相关性

唐琳, 李佳, 戴慧蓉, 李莉

上海交通大学附属第一人民医院, 上海 200080

收稿日期:2013-01-28 出版日期:2014-02-20 发布日期:2014-02-20
作者简介:唐琳, 女, 1978年生, 学士, 主管技师, 主要研究方向为肥大细胞及过敏性疾病。
基金资助:
国家自然科学基金面上项目(No.81072448、30972824、30671939);上海市科委创新行动计划 (No. 10495810200、11JC1410300)

Correlation between total IgE levels in cord blood and AICDA gene polymorphism

TANG Lin, LI Jia, DAI Huirong, LI Li.

Shanghai First People′s Hospital, Shanghai Jiaotong University, Shanghai 200080, China

Received:2013-01-28 Online:2014-02-20 Published:2014-02-20

摘要/Abstract

摘要： 目的探讨AICDA基因多态性与新生儿脐血总IgE水平的相关性。方法选取无过敏史足月产妇97例, 有过敏史足月产妇70例, 提取脐血基因组DNA, 采用聚合酶链反应-高温连接酶反应(PCR-LDR)检测AICDA基因rs2518144、rs2028373、rs11046349位点的基因型。同时, 采用电化学发光免疫分析法(ECLIA)检测新生儿脐血总IgE水平。结果新生儿脐血AICDA基因 rs2518144位点3组基因型(AA/AG/GG)及rs11046349位点3组基因型(TT/TG/GG)分布频率及等位基因频率在无过敏史组和有过敏史组产妇间差异无统计学意义(P均＞0.05)。rs2028373位点3组基因型(AA/AG/GG)及等位基因分布频率与对照组比较有明显差异(P值分别为0.020和0.006)。各位点基因型与脐血IgE水平无相关性(P均＞0.05)。结论新生儿脐血rs2028373基因多态性与母亲过敏史有一定相关性;但rs2518144、rs2028373、rs11046349位点基因型与脐血IgE水平无相关性。

关键词: 活化诱导胞嘧啶核苷脱氨酶, 免疫球蛋白E, 基因多态性, 脐血

Abstract: Objective To investigate the correlation between total immunoglobulin E(IgE) levels in cord blood and activation-induced cytidine deaminase(AICDA) gene polymorphism. Methods Cord blood samples of 70 infants whose mothers had allergic diseases and 97 infants whose mothers had no history of allergic diseases were collected. Genomic DNA were extracted from cord blood samples, and polymerase chain reaction-ligase detection reaction (PCR-LDR) was used to detect rs2518144, rs2028373, rs11046349 sites in AICDA gene. Meantime, total IgE levels in cord blood were detectedby electro-chemiluminescence immunoassay (ECLIA).Results There was no significant difference in distribution and allelic distribution frequencies of 3 rs2518144 genotypes (AA/AG/GG) and 3 rs11046349genotypes (TT/TG/GG) between allergic disease group and non-allergic disease group(P＞0.05).However, significant difference in genotype distribution and allelic distribution frequencies of 3 rs2028373genotypes (AA/AG/GG) between the 2 groups was detected(P=0.020 and 0.006). The genotyps of rs2518144, rs2028373 and rs11046349 sites were not related with total IgE levels(P＞0.05). Conclusions rs2028373 gene polymorphism is significantly correlated with allergic diseases, but the genotypes of rs2518144, rs2028373 and rs11046349 sites are not correlated with total IgE levels.

Key words: Activation-induced cytidine deaminase, Immunoglobulin E, Gene polymorphism, Cord blood

中图分类号:

Q503

唐琳, 李佳, 戴慧蓉, 李莉. 新生儿脐血IgE与AICDA基因多态性的相关性[J]. 检验医学, 2014, 29(2): 158-161.

TANG Lin, LI Jia, DAI Huirong, LI Li.. Correlation between total IgE levels in cord blood and AICDA gene polymorphism[J]. , 2014, 29(2): 158-161.

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新生儿脐血IgE与AICDA基因多态性的相关性

Correlation between total IgE levels in cord blood and AICDA gene polymorphism

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