脐血IL-13、AICDA和 TSLP基因位点与IgE的相关性

doi:10.3969/j.issn.1673-8640.2019.06.009

摘要/Abstract

摘要：

目的探讨新生儿脐血白细胞介素（IL）13、活化诱导胞嘧啶核苷脱氨酶（AICDA）和胸腺基质淋巴细胞生成素（TSLP）基因位点与免疫球蛋白E（IgE）水平的相关性。方法收集779名新生儿的脐血。提取脐血基因组DNA后采用聚合酶链反应-连接酶检测反应（PCR-LDR）检测IL-13、AICDA和TSLP基因的16个位点（IL-13基因rs20541、rs1800925、rs848、rs1295686和rs2069743位点;AICDA基因rs2518144、rs17792729、rs2580873、 rs2028373和rs7304723位点;TSLP基因rs3806933、rs1545169、rs764917、rs1837253、rs2289276和rs11466749位点）,同时检测脐血IgE水平。结果 IL-13基因的rs1295686位点（β=0.087,P=0.02）、AICDA基因的rs2028373位点（β=-0.082,P=0.02）和TSLP基因的rs2289276、rs764917、rs1837253位点（β值分别为-0.126、-0.083、0.188,P值分别为0.01、0.03、0.00）与脐血IgE相关。结论脐血IL-13基因rs1295686位点、AICDA基因rs2028373位点及TSLP基因rs2289276、rs764917、rs1837253位点可能是过敏易感基因位点。

关键词: 白细胞介素13基因, 活化诱导胞嘧啶核苷脱氨酶基因, 胸腺基质淋巴细胞生成素基因, 免疫球蛋白E, 脐血, 过敏性疾病

Abstract:

Objective To investigate the correlations between interleukin 13（IL-13）,activation-induced cytidine deaminase（AICDA） and thymic stromal lymphopoietin（TSLP） gene loci and immunoglobulin E（IgE） in cord blood. Methods Cord blood samples were collected from 779 neonates. Genomic DNA was extracted from cord blood samples,and polymerase chain reaction-ligase detection reaction（PCR-LDR） was used to detect IL-13 gene loci（rs20541,rs1800925,rs848,rs1295686 and rs2069743）,AICDA gene loci（rs2518144,rs17792729,rs2580873,rs2028373 and rs7304723） and TSLP gene loci（rs3806933,rs1545169,rs764917,rs1837253,rs2289276 and rs11466749）. Cord blood IgE level was determined. Results There were correlations between IL-13 gene locus rs1295686（β=0.087,P=0.02）,AICDA gene locus rs2028373（β=-0.082,P=0.02）,TSLP gene loci rs2289276,rs764917 and rs1837253（β=-0.126,-0.083 and 0.188,P=0.01,0.03 and 0.00,respectively） and cord blood IgE. Conclusions IL-13 gene locus rs1295686,AICDA gene locus rs2028373 and TSLP gene loci rs2289276,rs764917 and rs1837253 may be allergy susceptible loci.

Key words: Interleukin 13 gene, Activation-induced cytidine deaminase gene, Thymic stromal lymphopoietin gene, Immunoglobulin E, Cord blood, Allergic disease

中图分类号:

R446.1

唐琳, 朱晨琛, 张晓璟, 肖烨, 魏冰, 蔡眉, 唐振华. 脐血IL-13、AICDA和 TSLP基因位点与IgE的相关性[J]. 检验医学, 2019, 34(6): 522-525.

TANG Lin, ZHU Chenchen, ZHANG Xiaojing, XIAO Ye, WEI Bing, CAI Mei, TANG Zhenhua. Correlations between IL-13,AICDA and TSLP gene loci and IgE in cord blood[J]. Laboratory Medicine, 2019, 34(6): 522-525.

图/表 2

参考文献 24

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引物	上游引物序列（5'~3'）	下游引物序列（5'~3'）	长度（bp）
IL-13基因
rs20541	CCAGTTTGTAAAGGACCTGC	TGATGCTTTCGAAGTTTCAG	100
rs1800925	GGGTTTCTGGAGGACTTCTA	TGCAGCCATGTCGCCTTTTC	80
rs848	CAGTACTTATTACCAGGGAC	TTGGAGCCAAGGGTTCAGAG	97
rs1295686	AACTGCTGCAGGACAAAGAG	TGGAAAGCCCCTGGTTTGTG	100
rs2069743	CACTGTGAGAGGGATTGTCA	CCCAGAAAGACCTCTGAATC	99
AICDA基因
rs2518144	GCACTCACAGTTGACCATTA	TGGACTTTGGTTATCTTCGC	97
rs17792729	GCTGATGACTTGGGATTAC	GGGACTGAGGAACAAGTTTA	100
rs2580873	ATCAAGAGTCCCCATTGCAG	CAGGCATCACTTGTGCTTTC	99
rs2028373	ACTGAATTTTCATGCAGCCC	CTGCTGGAATACTTTTGTAG	92
rs7304723	AAGCCATCCTCCCTCCTCAA	TGATAAAGCTGCGAACCGTG	92
TSLP基因
rs3806933	TCAATTTCAGGAAAAACGCC	AGTGCTAGAGGACTCTTCAG	90
rs1545169	CTTACAGTTCTGAATGCTGG	AAGAGAGCCTTCACCAGGAC	89
rs764917	TGTAAAATCGCAAAGTAGG	GAACAGTTATTCCAAAAATGC	109
rs1837253	GGTTACTTTGTAAAAGATCC	CCTACTGGACTCTATTGTG	100
rs2289276	TGGCAATGAGAGGCAAAACC	CCACGAGTGTAAGAGATCTG	95
rs11466749	AGGGCCAACAGGTATAAAGG	GGGACATTATCTTCCTTCAG	101

引物	上游引物序列（5'~3'）	下游引物序列（5'~3'）	长度（bp）
IL-13基因
rs20541	CCAGTTTGTAAAGGACCTGC	TGATGCTTTCGAAGTTTCAG	100
rs1800925	GGGTTTCTGGAGGACTTCTA	TGCAGCCATGTCGCCTTTTC	80
rs848	CAGTACTTATTACCAGGGAC	TTGGAGCCAAGGGTTCAGAG	97
rs1295686	AACTGCTGCAGGACAAAGAG	TGGAAAGCCCCTGGTTTGTG	100
rs2069743	CACTGTGAGAGGGATTGTCA	CCCAGAAAGACCTCTGAATC	99
AICDA基因
rs2518144	GCACTCACAGTTGACCATTA	TGGACTTTGGTTATCTTCGC	97
rs17792729	GCTGATGACTTGGGATTAC	GGGACTGAGGAACAAGTTTA	100
rs2580873	ATCAAGAGTCCCCATTGCAG	CAGGCATCACTTGTGCTTTC	99
rs2028373	ACTGAATTTTCATGCAGCCC	CTGCTGGAATACTTTTGTAG	92
rs7304723	AAGCCATCCTCCCTCCTCAA	TGATAAAGCTGCGAACCGTG	92
TSLP基因
rs3806933	TCAATTTCAGGAAAAACGCC	AGTGCTAGAGGACTCTTCAG	90
rs1545169	CTTACAGTTCTGAATGCTGG	AAGAGAGCCTTCACCAGGAC	89
rs764917	TGTAAAATCGCAAAGTAGG	GAACAGTTATTCCAAAAATGC	109
rs1837253	GGTTACTTTGTAAAAGATCC	CCTACTGGACTCTATTGTG	100
rs2289276	TGGCAATGAGAGGCAAAACC	CCACGAGTGTAAGAGATCTG	95
rs11466749	AGGGCCAACAGGTATAAAGG	GGGACATTATCTTCCTTCAG	101