HBV前C区1896突变株感染慢性乙型肝炎患者外周血T淋巴细胞亚群的分析及意义

doi:10.3969/j.issn.1673-8640.2013.09.020

检验医学 ›› 2013, Vol. 28 ›› Issue (9): 815-819.DOI: 10.3969/j.issn.1673-8640.2013.09.020

HBV前C区1896突变株感染慢性乙型肝炎患者外周血T淋巴细胞亚群的分析及意义

上海市公共卫生临床中心医学检验科,上海 201508

收稿日期:2013-04-22 修回日期:2013-09-25 出版日期:2013-09-15 发布日期:2013-09-25
通讯作者: 陈戴红,联系电话:021-37990333-7350。
作者简介:毛会军,男,1979年生,硕士,主管技师,主要从事临床免疫学检测工作。

Analysis and significance of T lymphocyte subsets in peripheral blood of chronic hepatitis B patients with perC region 1896 mutation

MAO Huijun, QIU Jianping, LIN Feng, WANG Gang, ZHANG Min, CHEN Daihong

Department of Clinical Laboratory, Shanghai Public Health Clinical Center, Shanghai 201508,China

Received:2013-04-22 Revised:2013-09-25 Online:2013-09-15 Published:2013-09-25

摘要/Abstract

摘要：

目的探讨乙型肝炎病毒(HBV)前C区1896变异与感染宿主外周血T淋巴细胞亚群之间的关系。方法将196例乙型肝炎患者分为慢性乙型肝炎(CHB)组(146例)和乙型肝炎肝硬化(LC)组(50例),前者又分为含野生株CHB组(CHBⅠ组,80例)和前C区1896变异株CHB组(CHBⅡ组,66例);后者又分为含野生株LC组(LCⅠ组,19例)和前C区1896变异株LC组(LCⅡ组,31例)。采用特异性引物聚合酶链反应(PCR)检测196例乙型肝炎患者HBV前C区1896变异,并用流式细胞术检测外周血T淋巴细胞亚群的变化。以20名健康献血者作为正常对照组。结果 196例乙型肝炎患者中HBV 前C区1896变异检出率为49.5%;LC组中HBV前C区1896变异检出率为62.0%,高于CHB组(45.2%)。CHB 组CD4⁺T细胞绝对数明显低于正常对照组(P<0.01);LC组CD3⁺T细胞绝对数及百分率、CD8⁺T细胞绝对数、CD4⁺T细胞绝对数及百分率均明显低于正常对照组(P<0.01)。CHBⅡ组CD3⁺T细胞绝对数、CD4⁺T细胞绝对数均明显低于CHBⅠ组(P<0.05)。LCⅡ组CD3⁺T细胞绝对数、CD4⁺T细胞绝对数均明显低于CHBⅡ组(P<0.01)。结论 HBV前C区1896突变株感染的CHB患者较CHB野生株感染患者存在更为严重的T淋巴细胞亚群失衡,这种紊乱可能参与了HBV DNA前C区1896突变株感染导致的乙型肝炎慢性化过程。

关键词: T淋巴细胞亚群, 前C区1896变异, 乙型肝炎病毒, 慢性乙型肝炎

Abstract:

Objective To investigate the relationship of hepatitis B virus (HBV) preC region 1896 mutation with peripheral blood T lymphocyte subsets. Methods A total of 196 patients with hepatitis B were classified into 146 cases of chronic hepatitis B (CHB) group and 50 cases of hepatitis B liver cirrhosis (LC) group. CHB group was classified into 80 cases of wild-type strain CHB group (CHB Ⅰ group) and 66 cases of preC region 1896 mutation CHB group (CHB Ⅱ group). LC group was classified into 19 cases of wild-type strain LC group (LC Ⅰ group) and 31 cases of preC region 1896 mutation LC group (LC Ⅱ group). In 196 patients with hepatitis B, the specific primers of polymerase chain reaction (PCR) was used to detect the HBV preC region 1896 mutation, and the T lymphocyte subsets in peripheral blood were detected by flow cytometry. A total of 20 healthy subjects were enrolled as healthy control group. Results In 196 hepatitis B patients, the rate of HBV preC 1896 mutation was 49.5%. The mutation rate in LC group (62.0%) was higher than that in CHB group (45.2%). Compared with the healthy control group, the CD4⁺T cell absolute number of CHB group decreased significantly (P<0.01). In LC group, the absolute number and percentage of CD3⁺T cell, the absolute number of CD8⁺T cell and the absolute number and percentage of CD4⁺T cell were significantly lower than those in the healthy control group (P<0.01). Compared with CHB Ⅰ group, the CD3⁺T cell absolute number and CD4⁺T cell absolute number of CHB Ⅱ group decreased significantly (P<0.05). Compared with CHB Ⅱ group,the absolute number of CD3⁺T cell and CD4⁺T cell in LCⅡgroup had significant decrease (P<0.01). Conclusions In CHB patients with HBV preC 1896 mutation, the imbalance of T lymphocyte subsets is more serious than that of CHB patients with wild-type strain. The imbalance may participate in the pathogenesis of chronic hepatitis caused by the HBV preC region 1896 mutation.

Key words: T lymphocyte subset, perC region 1896 mutation, Hepatitis B virus, Chronic hepatitis B

中图分类号:

R446.63

毛会军,邱建萍,林锋,王刚,张敏,陈戴红. HBV前C区1896突变株感染慢性乙型肝炎患者外周血T淋巴细胞亚群的分析及意义[J]. 检验医学, 2013, 28(9): 815-819.

MAO Huijun, QIU Jianping, LIN Feng, WANG Gang, ZHANG Min, CHEN Daihong. Analysis and significance of T lymphocyte subsets in peripheral blood of chronic hepatitis B patients with perC region 1896 mutation[J]. , 2013, 28(9): 815-819.

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HBV前C区1896突变株感染慢性乙型肝炎患者外周血T淋巴细胞亚群的分析及意义

Analysis and significance of T lymphocyte subsets in peripheral blood of chronic hepatitis B patients with perC region 1896 mutation

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