16S rRNA甲基化酶基因在产ESBLs肠杆菌科细菌中的分布

doi:10.3969/j.issn.1673-8640.2013.09.010

检验医学 ›› 2013, Vol. 28 ›› Issue (9): 775-779.DOI: 10.3969/j.issn.1673-8640.2013.09.010

16S rRNA甲基化酶基因在产ESBLs肠杆菌科细菌中的分布

高辉,王杨,黄云昆,朱雯梅,王佳,姚瑶

昆明市延安医院检验科,云南昆明 650051

收稿日期:2012-10-10 修回日期:2013-09-25 出版日期:2013-09-15 发布日期:2013-09-25
通讯作者: 王杨,联系电话:0871-3211345。
作者简介:高辉,女,1963年生,学士,主任医师,主要从事临床微生物检验工作。

Distribution of 16S rRNA methylase genes in ESBLs-producing Enterobacteriaceae

GAO Hui,WANG Yang,HUANG Yunkun, ZHU Wenmei, WANG Jia, YAO Yao

Department of Clinical Laboratory,Kunming Yanan Hospital,Yunnan Kunming 650051,China

Received:2012-10-10 Revised:2013-09-25 Online:2013-09-15 Published:2013-09-25

摘要/Abstract

摘要：

目的了解临床分离的产超广谱β-内酰胺酶(ESBLs)的革兰阴性肠杆菌科细菌中16S rRNA甲基化酶基因的分布情况。方法对临床分离的70株革兰阴性肠杆菌科细菌用VITEK-32型全自动微生物分析系统进行细菌鉴定,用纸片扩散法检测ESBLs,并用聚合酶链反应(PCR)检测armA、rmtA、rmtB、rmtC、rmtD和npmA 6种16S rRNA甲基化酶基因,对检测的阳性产物进行测序,并通过GenBank比对DNA序列。结果 70株产ESBLs革兰阴性肠杆菌中,9株16S rRNA甲基化酶基因阳性,其中5株检出armA基因,4株检出rmtB基因,2株同时检出armA和rmtB基因,rmtA、rmtC、rmtD、npmA 4种基因扩增均为阴性。结论不同地区医院16S rRNA甲基化酶基因的分布情况各不相同。

关键词: 16S rRNA甲基化酶, 耐药基因, 革兰阴性肠杆菌科细菌

Abstract:

Objective To investigate the distribution of 16S rRNA methylase genes in extended-spectrum beta-lactamases (ESBLs)-producing Gram-negative Enterobacteriaceae. Methods A total of 70 isolates of Gram-negative Enterobacteriaceae were identified by VITEK-32 automated microbial system. The Kirby-Bauer method was used to detect ESBLs. Polymerase chain reaction(PCR) was used to detect 6 kinds of 16S rRNA methylase genes including armA, rmtA, rmtB, rmtC, rmtD and npmA. DNA sequencing was used to detect the positive productions of PCR with GenBank as control. Results From the 70 isolates of ESBLs-producing Gram-negative Enterobacteriaceae, 9 isolates carried 16S rRNA methylase genes, including 5 for armA gene, 4 for rmtB gene and 2 for both armA and rmtB genes, and no rmtA, rmtC, rmtD or npmA gene was detected. Conclusions The distribution of 16S rRNA methylase genes is various in the hospitals from different regions.

Key words: 16S rRNA methylase, Drug resistance gene, Gram-negative Enterobacteriaceae

中图分类号:

R378.2

高辉,王杨,黄云昆,朱雯梅,王佳,姚瑶. 16S rRNA甲基化酶基因在产ESBLs肠杆菌科细菌中的分布[J]. 检验医学, 2013, 28(9): 775-779.

GAO Hui,WANG Yang,HUANG Yunkun, ZHU Wenmei, WANG Jia, YAO Yao. Distribution of 16S rRNA methylase genes in ESBLs-producing Enterobacteriaceae[J]. , 2013, 28(9): 775-779.

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16S rRNA甲基化酶基因在产ESBLs肠杆菌科细菌中的分布

Distribution of 16S rRNA methylase genes in ESBLs-producing Enterobacteriaceae

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