青浦区266株结核分枝杆菌基因分型及其与耐药表型关系研究

doi:10.3969/j.issn.1673-8640.2015.10.004

摘要/Abstract

摘要： 目的

探讨上海市青浦区流行的结核分枝杆菌(MTB)的主要基因型及其与耐药表型的关系。

方法

分别提取266株MTB DNA,应用多重聚合酶链反应(PCR)对其进行分型(北京基因型/非北京基因型)鉴定;采用比例法对此266株菌株进行利福平、异烟肼、链霉素和乙胺丁醇的药物敏感性试验;运用SPSS 17.0统计软件分析基因分型与患者性别、年龄的关系,以及基因分型与菌株耐药性之间的关系。

结果

266株菌株中北京基因型242株(90.98%),非北京基因型24株(9.02%)。北京基因型菌株的总耐药率为23.96%,耐多药率为9.91%,非北京基因型的总耐药率和耐多药率分别为29.16%和8.33%,两者差异无统计学意义(P均>0.05)。

结论

北京基因型MTB可能是上海市青浦区的主要流行株,尚未发现北京基因型菌株和非北京基因型菌株在耐药方面有明显差异。

关键词: 结核分枝杆菌, 北京基因型, 耐药

Abstract: Objective

To investigate the genotype and relationship of genotype with drug-resistant phenotypes of Mycobacterium tuberculosis(MTB)popular isolates in Qingpu, Shanghai.

Methods

The DNA of 266 MTB isolates were extracted and identified as Beijing genotype and non-Beijing genotype by multiplex polymerase chain reaction (PCR). Drug sensitivity tests of the 266 MTB isolates against streptomycin, isoniazid, rifampicin and ethambutol were performed by proportion method. The relationships of genotype with sex, age and drug-resistant phenotypes were analyzed by SPSS 17.0 statistical software, respectively.

Results

The 242 isolates (90.98%) were identified as Beijing genotype, while 24 isolates (9.02%) were identified as non-Beijing genotype. The overall drug resistance rate of Beijing genotype isolates was 23.96%, while multidrug-resistance rate was 9.91%.The overall drug resistance rate and multidrug-resistance rate were 29.16% and 8.83% as to non-Beijing genotype.

Conclusions

The MTB popular isolates of Qingpu, Shanghai might be Beijing genotype, but it exists significant difference between Beijing and non-Beijing genotypes for drug resistance.

Key words: Mycobacterium tuberculosis, Beijing genotype, Drug resistance

中图分类号:

R378.91

彭荣, 费凤英. 青浦区266株结核分枝杆菌基因分型及其与耐药表型关系研究[J]. 检验医学, 2015, 30(10): 983-986.

PENG Rong, FEI Fengying.. Research on genotype and relationship of genotype with drug-resistant phenotypes from 266 Mycobacterium tuberculosis in Qingpu District[J]. Laboratory Medicine, 2015, 30(10): 983-986.

图/表 3

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组别	例数	性别		年龄(岁)
组别	例数	男性	女性	≤30	31~59	≥60
北京基因型组	242	165(90.66)	77(91.67)	43(89.58)	123(91.11)	76(91.57)
非北京基因型组	24	17(9.34)	7(8.33)	5(10.42)	12(8.89)	7(8.43)
χ²值		1.961		6.547
P值		0.215		0.392

组别	例数	性别		年龄(岁)
组别	例数	男性	女性	≤30	31~59	≥60
北京基因型组	242	165(90.66)	77(91.67)	43(89.58)	123(91.11)	76(91.57)
非北京基因型组	24	17(9.34)	7(8.33)	5(10.42)	12(8.89)	7(8.43)
χ²值		1.961		6.547
P值		0.215		0.392

组别	例数	总耐药情况			单药耐药情况
组别	例数	全敏感	耐药	耐多药	链霉素	利福平	异烟肼	乙胺丁醇
北京基因型组	242	184(76.03)	58(23.96)	24(9.91)	23(9.51)	19(7.85)	14(5.78)	41(16.94)
非北京基因型组	24	17(70.88)	7(29.16)	2(8.33)	3(12.5)	2(8.33)	2(8.33)	4(16.66)
χ²值			1.892	2.176	0.994	2.227	0.261	0.993
P值			0.511	0.823	0.432	0.641	0.159	0.915

组别	例数	总耐药情况			单药耐药情况
组别	例数	全敏感	耐药	耐多药	链霉素	利福平	异烟肼	乙胺丁醇
北京基因型组	242	184(76.03)	58(23.96)	24(9.91)	23(9.51)	19(7.85)	14(5.78)	41(16.94)
非北京基因型组	24	17(70.88)	7(29.16)	2(8.33)	3(12.5)	2(8.33)	2(8.33)	4(16.66)
χ²值			1.892	2.176	0.994	2.227	0.261	0.993
P值			0.511	0.823	0.432	0.641	0.159	0.915