脑胶质瘤组织lncRNA SOX21-AS1、miR-875-5p表达及其与患者预后的关系

doi:10.3969/j.issn.1673-8640.2024.03.001

摘要/Abstract

摘要：

目的探讨脑胶质瘤患者癌组织长链非编码RNA（lncRNA）SRY-box转录因子21反义RNA 1（SOX21-AS1）、miR-875-5p表达及其与患者预后的关系。方法选取2014年7月—2018年8月三门峡市中心医院脑胶质瘤患者77例（胶质瘤组），以颅脑损伤患者50例作为对照组。收集2个组经手术切除的脑胶质瘤组织和正常脑组织，测定组织中lncRNA SOX21-AS1、miR-875-5p相对表达量。采用Pearson相关分析评估lncRNA SOX21-AS1和miR-875-5p的相关性。采用Kaplan-Meier生存曲线分析脑胶质瘤患者的预后情况。采用Cox回归分析评估脑胶质瘤患者预后的影响因素。结果胶质瘤组lncRNA SOX21-AS1相对表达量高于对照组（P<0.001），miR-875-5p相对表达量低于对照组（P<0.001）。不同世界卫生组织（WHO）分级的脑胶质瘤患者lncRNA SOX21-AS1、miR-875-5p相对表达量差异有统计学意义（P<0.05）。Pearson相关分析结果显示，脑胶质瘤组织中lncRNA SOX21-AS1相对表达量与miR-875-5p相对表达量呈负相关（r=-0.554，P<0.001）。Kaplan-Meier生存曲线分析结果显示，lncRNA SOX21-AS1低表达组、miR-875-5p高表达组累积生存率分别高于lncRNA SOX21-AS1高表达组、miR-875-5p低表达组（P<0.05）。多因素Cox回归分析结果显示，WHO分级和lncRNA SOX21-AS1均是脑胶质瘤患者不良预后的独立危险因素[风险比（HR）值分别为2.266、2.390，95%可信区间（CI）分别为1.452~3.536、1.496~3.818，P<0.001]。结论 lncRNA SOX21-AS1和miR-875-5p均与脑胶质瘤患者预后密切相关，或可作为评估脑胶质瘤患者预后的有效指标。

关键词: 长链非编码RNA SRY-box转录因子21反义RNA 1, miR-875-5p, 脑胶质瘤, 预后

Abstract:

Objective To investigate the expression levels of long non-coding RNA（lncRNA）SRY-box transcription factor 21 antisense RNA 1（SOX21-AS1）and miR-875-5p in glioma tissues and the relationship with the prognosis of patients. Methods Totally，77 patients with glioma in Sanmenxia Central Hospital from July 2014 to August 2018 were enrolled as glioma group，and 50 patients with brain injury were enrolled as control group. The glioma tissues and normal brain tissues that surgically removed in the 2 groups were taken，and the expression levels of lncRNA SOX21-AS1 and miR-875-5p were determined. Pearson correlation analysis was used to analyze the correlation between the expression levels of lncRNA SOX21-AS1 and miR-875-5p in glioma tissues. Kaplan-Meier curve was used to analyze the prognosis of patients with glioma. Cox regression analysis was used to analyze the factors for the prognosis of patients with glioma. Results The expression level of lncRNA SOX21-AS1 in glioma group was higher than that in control group（P<0.001），and the expression level of miR-875-5p was lower（P<0.001）. The expression levels of lncRNA SOX21-AS1 and miR-875-5p in glioma tissue had statistical significance with different World Health Organization（WHO）classifications（P<0.05）. The expression level of lncRNA SOX21-AS1 in glioma tissue was negatively correlated with miR-875-5p（r=-0.554，P<0.001）. The cumulative survival rate of patients with glioma in lncRNA SOX21-AS1 low expression group and miR-875-5p high expression group was higher than that in lncRNA SOX21-AS1 high expression group and miR-875-5p low expression group（P<0.05）. WHO classification and lncRNA SOX21-AS1 were independent risk factors that affected the poor prognosis of patients with glioma [hazard ratios（HR）were 2.266 and 2.390，95% confidence intervals（CI）were 1.452-3.536 and 1.496-3.818，P<0.001]. Conclusions The expressions of lncRNA SOX21-AS1 and miR-875-5p are related to the prognosis of patients with glioma，and both can be used as effective indicators to assess the prognosis of patients with glioma.

Key words: Long non-coding RNA SRY-box transcription factor 21 antisense RNA 1, MiR-875-5p, Glioma, Prognosis

中图分类号:

R446.1

方丹东, 程岗, 黄伟, 刘晓楠, 毛尖, 侯保森, 刘十岷. 脑胶质瘤组织lncRNA SOX21-AS1、miR-875-5p表达及其与患者预后的关系[J]. 检验医学, 2024, 39(3): 209-214.

FANG Dandong, CHENG Gang, HUANG Wei, LIU Xiaonan, MAO Jian, HOU Baosen, LIU Shimin. Expression of lncRNA SOX21-AS1 and miR-875-5p in glioma tissues and relationship with prognosis of patients[J]. Laboratory Medicine, 2024, 39(3): 209-214.

图/表 9

参考文献 14

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基因	正向引物（5'~3'）	反向引物（5'~3'）
lncRNA SOX21-AS1	GGCTCAGGTTTAGGCGAGTG	AGAGGCTCTCCTACTCGTGC
GAPDH	AGCCACATCGCTCAGACAC	GCCCAATACGACCAAATCC
miR-875-5p	CACATGGACATACGGGTT	CATCTCCGATTTGTTCCCT
U6	CTTAGTTGCATGCAG	AATCGTGTATAAGTC

基因	正向引物（5'~3'）	反向引物（5'~3'）
lncRNA SOX21-AS1	GGCTCAGGTTTAGGCGAGTG	AGAGGCTCTCCTACTCGTGC
GAPDH	AGCCACATCGCTCAGACAC	GCCCAATACGACCAAATCC
miR-875-5p	CACATGGACATACGGGTT	CATCTCCGATTTGTTCCCT
U6	CTTAGTTGCATGCAG	AATCGTGTATAAGTC

组别	例数	lncRNA SOX21-AS1	miR-875-5p
对照组	50	1.06±0.35	1.03±0.34
胶质瘤组	77	2.28±0.76	0.43±0.14
t值		10.631	13.809
P值		<0.001	<0.001

组别	例数	lncRNA SOX21-AS1	miR-875-5p
对照组	50	1.06±0.35	1.03±0.34
胶质瘤组	77	2.28±0.76	0.43±0.14
t值		10.631	13.809
P值		<0.001	<0.001

自变量	单因素分析		多因素分析
自变量	HR值（95%CI）	P值	HR值（95%CI）	P值
性别	1.008（0.825~1.231）	0.938
病理类型	1.025（0.838~1.254）	0.811
肿瘤位置	0.989（0.811~1.206）	0.913
年龄	1.103（0.896~1.358）	0.355
WHO分级	2.457（1.517~3.979）	<0.001	2.266（1.452~3.536）	<0.001
肿瘤大小	1.061（0.864~1.303）	0.573
lncRNA SOX21-AS1	2.582（1.557~4.281）	<0.001	2.390（1.496~3.818）	<0.001
miR-875-5p	0.678（0.556~0.826）	<0.001	0.894（0.731~1.094）	0.277