血清外泌体、胎盘来源外泌体和外泌体miR-210与子痫前期的相关性

doi:10.3969/j.issn.1673-8640.2022.08.003

检验医学 ›› 2022, Vol. 37 ›› Issue (8): 715-719.DOI: 10.3969/j.issn.1673-8640.2022.08.003

血清外泌体、胎盘来源外泌体和外泌体miR-210与子痫前期的相关性

段玉萍¹, 谢骊¹, 蔡雷鸣¹, 吴晶晶², 洪茂¹, 厉倩¹()

1.上海市宝山区吴淞中心医院检验科,上海 200940
2.上海市宝山区吴淞中心医院妇产科,上海 200940

收稿日期:2021-05-16 修回日期:2022-04-25 出版日期:2022-08-30 发布日期:2022-09-16
通讯作者: 厉倩
作者简介:厉倩,E-mail： aqianmerry@163.com。
段玉萍,女,1989年生,硕士,主管技师,主要从事子痫前期病理机制研究。
基金资助:
上海市宝山区科技创新专项(2018-E-5)

Correlation of serum exosomes,placental exosomes and exosomal miR-210 with preeclampsia

DUAN Yuping¹, XIE Li¹, CAI Leiming¹, WU Jingjing², HONG Mao¹, LI Qian¹()

1. Department of Clinical Laboratory,Wusong Central Hospital,Shanghai 200940,China
2. Department of Gynaecology and Obstetrics,Wusong Central Hospital,Shanghai 200940,China

Received:2021-05-16 Revised:2022-04-25 Online:2022-08-30 Published:2022-09-16
Contact: LI Qian

摘要/Abstract

摘要：

目的探讨血清外泌体、胎盘来源外泌体[胎盘碱性磷酸酶（PLAP）]和外泌体微小RNA-210（miR-210）在子痫前期（PE）患者中的变化及临床意义。方法选取PE患者30例（PE组）和健康孕妇30例（正常妊娠组）,收集所有对象孕早期、孕中期和孕晚期的血清样本,检测血清外泌体数量、PLAP水平和外泌体miR-210相对表达量。采用Pearson相关分析评估PLAP与miR-210的相关性。结果正常妊娠组和PE组血清外泌体数量、PLAP水平和外泌体miR-210相对表达量均随孕期逐步升高。PE组不同孕期血清外泌体数量、PLAP水平和外泌体miR-210相对表达量均高于正常妊娠组（P<0.05）。PLAP水平与外泌体miR-210相对表达量呈正相关（r=0.668,P<0.01）。结论 PE患者血清外泌体数量、PLAP水平和外泌体miR-210相对表达量在孕早期已发生变化,这3项指标或可作为预测和早期诊断PE的生物标志物。

关键词: 外泌体, 胎盘碱性磷酸酶, 微小RNA-210, 子痫前期

Abstract:

Objective To investigate the changes and roles of serum exosomes,placental exosomes [placental alkaline phosphatase（PLAP）] and exosomal microRNA-210（miR-210） in pregnant women during preeclampsia（PE） pregnancy. Methods Totally,30 patients with PE and 30 healthy pregnant women were enrolled. All serum samples were collected in the first trimester,the second trimester and the third trimester of pregnancy. The amount of serum exosomes,the level of PLAP and the expression of exosomal miR-210 were determined. The correlation between PLAP and miR-210 was analyzed using Pearson analysis. Results The amount of serum exosomes,the level of PLAP and miR-210 were increased with the progress of pregnancy. Compared with healthy pregnant women,the amount of serum exosomes,the level of PLAP and miR-210 in PE group were higher in different trimesters of pregnancy（P<0.05）. The correlation between the level of PLAP and exosomal miR-210 was positive（r=0.668,P<0.01）. Conclusions The amount of serum exosomes,the level of PLAP and exosomal miR-210 in PE group change from the first trimester of pregnancy,and they play roles in the early prediction and diagnosis of PE.

Key words: Exosome, Placental alkaline phosphatase, MicroRNA-210, Preeclampsia

中图分类号:

R446.1

段玉萍, 谢骊, 蔡雷鸣, 吴晶晶, 洪茂, 厉倩. 血清外泌体、胎盘来源外泌体和外泌体miR-210与子痫前期的相关性[J]. 检验医学, 2022, 37(8): 715-719.

DUAN Yuping, XIE Li, CAI Leiming, WU Jingjing, HONG Mao, LI Qian. Correlation of serum exosomes,placental exosomes and exosomal miR-210 with preeclampsia[J]. Laboratory Medicine, 2022, 37(8): 715-719.

图/表 5

参考文献 19

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组别	例数	孕早期/（×10⁹/mL）	孕中期/（×10⁹/mL）	孕晚期/（×10⁹/mL）
正常妊娠组	30	0.885 1±0.397 4	1.057 4±0.390 1	1.202 0±0.398 6^*
PE组	30	0.993 3±0.416 6^#	1.248 3±0.341 6^*#	1.525 1±0.474 1^*#

组别	例数	孕早期/（×10⁹/mL）	孕中期/（×10⁹/mL）	孕晚期/（×10⁹/mL）
正常妊娠组	30	0.885 1±0.397 4	1.057 4±0.390 1	1.202 0±0.398 6^*
PE组	30	0.993 3±0.416 6^#	1.248 3±0.341 6^*#	1.525 1±0.474 1^*#

组别	例数	孕早期/（pg/mL）	孕中期/（pg/mL）	孕晚期/（pg/mL）
正常妊娠组	30	579.48±216.15	711.08±377.61	764.00±403.98^*
PE组	30	740.19±350.23^#	934.15±377.93^*#	1 065.83±543.79^*#

组别	例数	孕早期/（pg/mL）	孕中期/（pg/mL）	孕晚期/（pg/mL）
正常妊娠组	30	579.48±216.15	711.08±377.61	764.00±403.98^*
PE组	30	740.19±350.23^#	934.15±377.93^*#	1 065.83±543.79^*#

组别	例数	孕早期	孕中期	孕晚期
正常妊娠组	30	8.14±1.48	7.84±1.53	6.52±1.78^*
PE组	30	6.90±1.72^#	5.22±1.62^*#	4.56±1.59^*#

血清外泌体、胎盘来源外泌体和外泌体miR-210与子痫前期的相关性

Correlation of serum exosomes,placental exosomes and exosomal miR-210 with preeclampsia

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