关键词: 自动化, 细菌学技术, 接种, 培养技术, 数字成像

Abstract:

Objective To compare the bacterial separation effects of Diascie ProBact automated microbial inoculation system,Copan Wasp automated microbial pretreatment system and manual method,and to evaluate the clinical application significance of Diascie ProBact automated microbial inoculation system. Methods A total of 50 clinical specimens of sputum and 50 clinical specimens of mid-stream urine were inoculated by Diascie ProBact,Copan Wasp and manual method. The sputum specimens were inoculated with a quadrant streaking and incubated at 35 ℃ in 5% CO₂ incubator. Mid-stream urine specimens were inoculated with a continuous streaking and incubated at 35 ℃ in common incubator. The bacterial growth of the 2 types of specimens was observed after 24 h. The number of isolate species and single colony isolates and the growth of bacterial colony were analyzed and compared. Results Of 0,1,2 and ≥3 isolate species in the sputum specimens,1,16,8 and 25 cases were isolated by Diascie ProBact,1,14,10 and 25 cases by Copan Wasp,and 2,14,9 and 25 cases by manual method,respectively. Of 0,1,2 and ≥3 isolate species inthe mid-stream urine specimens,20,16,8 and 6 cases were isolated by Diascie ProBact,18,17,9 and 6 cases by Copan Wasp,and 19,18,7 and 6 cases by manual method,respectively. There was no statistical significance among the 3 methods （P>0.05）. Of ≤10×10³,100×10³,1 000×10³ and ≥10 000×10³ cfu/mL bacterial quantities in the sputum specimens,4,5,8 and 33 cases were isolated by Diascie ProBact,5,5,9 and 31 cases by Copan Wasp,and 6,7,10 and 27 cases by manual method,respectively. Of 0,10³-10⁴,10⁴-10⁵ and >10⁵ cfu/mL bacterial quantities in the mid-stream urine specimens,20,8,5 and 17 cases were isolated by Diascie ProBact,18,9,6 and 17 cases by Copan Wasp,and 19,7,6 and 18 cases by manual method,respectively. There was no statistical significance among the 3 methods（P>0.05）. In the sputum specimens,the single colony of Diascie ProBact was 9.78±5.37,that of Copan Wasp was 10.48±5.59,and that of manual method was 8.82±5.31. In the mid-stream urine specimens,the single colony of Diascie ProBact was 8.78±4.38,that of Copan Wasp was 9.74±4.49,and that of manual method was 7.33±5.03. There was no statistical significance among the 3 methods for sputum and mid-stream urine specimens （P=0.310 for sputum specimens and P=0.131 for mid-stream urine specimens）. Conclusions The performance of Diascie ProBact is not different from those of Copan Wasp and manual method. It could be used to clinical practice for improving the efficiency of colony separation,reducing the frequency of re-separation and shortening the time of report.

Key words: Automation, Bacteriological technique, Inoculation, Culturing technique, Digital imaging