检验医学 ›› 2016, Vol. 31 ›› Issue (9): 807-809.DOI: 10.3969/j.issn.1673-8640.2016.09.016

• 技术研究与评价·论著 • 上一篇    下一篇

SYSMEX XE-5000全自动血液分析仪在儿童脑脊液常规检测中的应用

孙奎霞, 杨文双, 蒋世菊   

  1. 北京大学第一医院检验科,北京 100034
  • 收稿日期:2015-08-04 出版日期:2016-09-30 发布日期:2016-10-11
  • 作者简介:null

    作者简介:孙奎霞,女,1985年生,博士,助理研究员,主要从事临床检验工作。

Application of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children

SUN Kuixia, YANG Wenshuang, JIANG Shiju   

  1. Department of Clinical Laboratory,Peking University First Hospital,Beijing 100034,China
  • Received:2015-08-04 Online:2016-09-30 Published:2016-10-11

摘要:

目的探讨SYSMEX XE-5000全自动血液分析仪(简称XE-5000)在儿童脑脊液常规检测中的应用价值。方法选取194份住院患儿的脑脊液标本,采用XE-5000体液模式进行脑脊液常规检测,同时行人工显微镜镜检,比较仪器法与人工镜检法检测结果的符合性。结果当白细胞(WBC)计数≥30个/μL时,2种方法差异无统计学意义(P>0.05)。当WBC计数<30个/μL时,2种方法差异有统计学意义(P<0.05)。当WBC计数≥10个/μL时,2种方法之间单个核细胞计数及多个核细胞计数均具有良好的相关性(P<0.01)。当红细胞(RBC)计数>1 000个/μL时,2种方法具有良好的相关性(r=0.98,P<0.01)。结论应用XE-5000体液模式检测儿童脑脊液,在WBC总数≥30个/μL时具有较好的应用价值,可代替人工镜检。

关键词: 细胞计数, 脑脊液, 儿童, SYSMEX XE-5000全自动血液分析仪

Abstract:

Objective To investigate the significance of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children. Methods A total of 194 cerebrospinal fluid samples were collected and determined by XE-5000 and manual microscopy. The consistency was evaluated. Results When white blood cell(WBC) count was ≥30 cells/μL,there was no statistical significance between the 2 methods(P>0.05). However,when WBC count was <30 cells/μL,there was a statistical significance between the 2 methods(P<0.05). When WBC count was ≥10 cells/μL,there were good correlations in counting mononuclear cells and multinuclear cells between the 2 methods (P<0.01). When red blood cell(RBC) count was >1 000 cells/μL,there was a good correlation between the 2 methods(r=0.98,P<0.01). Conclusions XE-5000 can be used in the determination of cerebrospinal fluid among children,when WBC count is ≥30 cells/μL.

Key words: Cell counting, Cerebrospinal fluid, Children, SYSMEX XE-5000 automatic blood analyzer

中图分类号: