基于核酸扩增技术的分枝杆菌检测及耐药分析技术的发展

doi:10.3969/j.issn.1673-8640.2016.05.018

摘要/Abstract

摘要：

分子生物学的迅猛发展和结核分枝杆菌等多种分枝杆菌基因组序列的破译,使得应用分子生物学方法对结核分枝杆菌复合群（MTBC）进行检测、耐药分析及对分枝杆菌属进行菌种鉴定成为了现实。核酸扩增（NAA）技术是分子生物学研究的基础,近年来,国内外多种基于此技术用于结核病、分枝杆菌病等相关疾病病原菌检测的方法也得到了验证和评估。文章综述了国内外这些方法的发展现状,并对NAA技术在分枝杆菌检测及耐药分析的临床应用可行性进行简评及展望。

关键词: 核酸扩增技术, 结核分枝杆菌, 分枝杆菌, 耐药性

Abstract:

With the rapid development of molecular biology and genome sequence decryption of Mycobacterium,especially Mycobacterium tuberculosis complex （MTBC）,it is possible to detect and identify Mycobacterium and analyze drug resistance by molecular biological methods. Nucleic acid amplification （NAA） techniques are the basis of molecular biology. Recently,many kinds of Mycobacterium identification methods based on NAA techniques are established and evaluated. This paper reviews the progress of these methods and assesses their clinical application prospects in Mycobacterium detection and drug resistance analysis.

Key words: Nucleic acid amplification techniques, Mycobacterium tuberculosis, Mycobacterium, Drug resistance

中图分类号:

R446.5

刘旻雁, 白冰, 陈晋. 基于核酸扩增技术的分枝杆菌检测及耐药分析技术的发展[J]. 检验医学, 2016, 31(5): 426-432.

LIU Minyan, BAI Bing, CHEN Jin. Progress of nucleic acid amplification techniques in Mycobacterium detection and drug resistance analysis[J]. Laboratory Medicine, 2016, 31(5): 426-432.

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