检验医学 ›› 2016, Vol. 31 ›› Issue (5): 383-386.DOI: 10.3969/j.issn.1673-8640.2016.05.009

• 临床应用研究·论著 • 上一篇    下一篇

替加环素对不同耐药基因型耐碳青霉烯类肠杆菌科细菌的敏感性研究

刘立荣1, 李向阳2, 瞿玲娜1, 唐玉霞1, 曹景宏1, 颜小萍1   

  1. 1.台州市中西医结合医院检验科,浙江 台州 317523
    2.温州医科大学附属第二医院检验科,浙江 温州 325027
  • 收稿日期:2015-12-29 出版日期:2016-05-30 发布日期:2016-06-13
  • 作者简介:null

    作者简介:刘立荣,男,1970年生,副主任技师,主要从事临床医学检验工作。

Sensitivity of carbapenem-resistant Enterobacteriaceae with different resistant genotypes against tigecycline

LIU Lirong1, LI Xiangyang2, QU Lingna1, TANG Yuxia1, CAO Jinghong1, YAN Xiaoping1   

  1. 1. Department of Clinical Laboratory,Chinese and Western Combined Hospital of Taizhou,Taizhou 317523,Zhejiang,China
    2. Department of Clinical Laboratory,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,Zhejiang,China
  • Received:2015-12-29 Online:2016-05-30 Published:2016-06-13

摘要:

目的 探讨替加环素对不同耐药基因型耐碳青霉烯类肠杆菌科细菌(CRE)的敏感性。方法 采用改良Hodge试验(MHT)筛查厄它培南耐药的肠杆菌科细菌,采用纸片扩散法检测替加环素的抑菌环直径,采用聚合酶链反应(PCR)测定CRE的β-内酰胺酶基因种类。结果 68株CRE中有48株(70.6%)MHT初筛阳性,其中40株(83.3%)携带能水解碳青霉烯类抗菌药物的超广谱β-内酰胺酶基因(KPCNDM-1、IMP-8、IMP-4,以KPC为最多,占67.5%)。68株菌株中有54株对替加环素敏感,12株中介,2株耐药,总敏感性为97%。MHT筛查试验阳性和阴性菌株对替加环素的敏感性差异无统计学意义(χ2=3.599,P>0.05)。结论 替加环素对CRE显示出优异的抗菌活性,其抗菌作用不受β-内酰胺酶的影响。

关键词: 耐碳青霉烯类肠杆菌科细菌, 替加环素, 敏感性

Abstract:

Objective To investigate the sensitivity of carbapenem-resistant Enterobacteriaceae(CRE)with different resistant genotypes against tigecycline. Methods The isolates of Enterobacteriaceae against ertapenem were screened by modified Hodge test (MHT),the diameters of tigecycline inhibition zones were determined by K-B method, and beta-lactamase gene of CRE was determined by polymerase chain reaction(PCR). Results A total of 68 isolates of CRE were screened by MHT,and there were 48 positive isolates,accounting for 70.6%. In the 48 positive isolates, there were 40 isolates (83.3%) carrying hydrolyzed carbapenem antibiotics extended-spectrum beta-lactamase gene, including KPC,NDM-1,IMP-8 and IMP-4. KPC was the most,accounting for 67.5%. In the 68 isolates of CRE,54 isolates were sensitive,12 isolates were intermediary,2 isolates were resistant,and the total sensitivity was 97%. There was no statistical significance for the sensitivity of positive and negative isolates in the 68 isolates of CRE by MHT (χ2=3.599,P>0.05). Conclusions Tigecycline for CRE exhibits excellent antibacterial activity. Antibacterial effect of tigecycline is not influenced by enzyme activity.

Key words: Carbapenem-resistant Enterobacteriaceae, Tigecycline, Sensitivity

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