转录因子Flo8 G723R、T751D突变增强白念珠菌毒力

doi:10.3969/j.issn.1673-8640.2016.011.014

检验医学 ›› 2016, Vol. 31 ›› Issue (11): 987-992.DOI: 10.3969/j.issn.1673-8640.2016.011.014

转录因子Flo8 G723R、T751D突变增强白念珠菌毒力

李文静¹, 沈韻¹, 刘锦燕², 史册¹, 王影¹, 赵悦¹, 项明洁²

1.上海交通大学医学院附属瑞金医院检验科,上海 200025
2.上海交通大学医学院附属瑞金医院卢湾分院放免检验科,上海 200020

收稿日期:2016-01-08 出版日期:2016-11-30 发布日期:2016-12-22
作者简介:null
作者简介：李文静,女,1991年生,学士,主要从事微生物学研究。沈韻,女,1967年生,主管技师,主要从事临床检验工作。沈韻与李文静对本文具有同等贡献,为并列第一作者。

通讯作者：项明洁,联系电话：021-63867643。
基金资助:
上海市科学技术委员会基金项目（15ZR1426900）;上海市医学重点专科项目（ZK2012A21）;上海市黄浦区优秀青年人才项目（RCPY1407）

Transcription factor Flo8 G723R and T751D mutations enhancing virulence in Candida albicans

LI Wenjing¹, SHEN Yun¹, LIU Jinyan², SHI Ce¹, WANG Ying¹, ZHAO Yue¹, XIANG Mingjie²

1. Department of Clinical Laboratory,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China
2. Radioimmunology and Clinical Laboratory,Luwan Branch,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200020,China

Received:2016-01-08 Online:2016-11-30 Published:2016-12-22

摘要/Abstract

摘要：

目的研究白念珠菌转录因子Flo8 G723R和T751D突变与毒力的关系。方法比较白念珠菌Flo8野生株SN152、Flo8回复株Flo8^SN152、Flo8突变株Flo8^G723R和Flo8^T751D感染小鼠的致死率和肾脏菌量负担,同时检测各菌株毒力因子的基因表达情况,分析Flo8突变与白念珠菌毒力的关系。结果与SN152和Flo8^SN152相比,转录因子Flo8突变株Flo8^G723R和Flo8^T751D感染小鼠的致死率更高、肾脏菌量负担更高,毒力因子的基因表达也有不同程度的增强。结论转录因子Flo8 G723R和T751D突变使白念珠菌毒力增加,部分毒力因子的基因表达增强。

关键词: G723R, T751D, 毒力, 转录因子Flo8, 白念珠菌

Abstract:

Objective To study the relationship between Candida albicans transcription factor Flo8 G723R and T751D mutations and their virulence. Methods The lethality rate,renal fungal burden of infected mice and the expression of genes for virulence factors in Candida albicans Flo8 wild-type SN152,Flo8 complementation strain Flo8^SN152,Flo8 mutation strains Flo8^G723R and Flo8^T751D were compared. The relationship between Flo8 mutations and Candida albicans virulence was analyzed. Results Compared to SN152 and Flo8^SN152,Flo8 mutation strains Flo8^G723R and Flo8^T751D produced a higher lethality rate and more renal fungal burden of infected mice and the expression of genes for virulence factors,and increased at different degrees. Conclusions Transcription factor Flo8 G723R and T751D mutations increase the virulence and the expression of genes for partial virulence factors in Candida albicans.

Key words: G723R, T751D, Virulence, Transcription factor Flo8, Candida albicans

中图分类号:

R519.5

李文静, 沈韻, 刘锦燕, 史册, 王影, 赵悦, 项明洁. 转录因子Flo8 G723R、T751D突变增强白念珠菌毒力[J]. 检验医学, 2016, 31(11): 987-992.

LI Wenjing, SHEN Yun, LIU Jinyan, SHI Ce, WANG Ying, ZHAO Yue, XIANG Mingjie. Transcription factor Flo8 G723R and T751D mutations enhancing virulence in Candida albicans[J]. Laboratory Medicine, 2016, 31(11): 987-992.

图/表 5

参考文献 23

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引物	序列（5'~3'）
ALS1-F	CATTTTCCCGTCCTCATT
ALS1-R	CATTGCTATCTGGATTTGTG
ALS5-F	AGTTATGGGGATGTTGCT
ALS5-R	CTGGTTATTATCTGAGGG
HWP1-F	AGGTAGACGGTCAAGGTG
HWP1-R	TTGTGGCTGTTGTGGATA
INT1-F	CATTTTAGCCAACTCGTC
INT1-R	GAATCAGTTTCGTGCTCT
MNT1-F	GTCCGTGAACAAATGAGA
MNT1-R	GGTAAGGAAATAGTGAATGC
SAP2-F	CTACTCCAACAACAACCA
SAP2-R	CATCAGGAACCCATAAATC
PLB1-F	TATCTCAAGGTTTGTGGG
PLB1-R	ATAAGCATCACCTTCGTC
18S RNA-F	TCTTGTACGGCACATATCTCGT
18S RNA-R	GTTTCGAGAGGACCTTCAGTC

引物	序列（5'~3'）
ALS1-F	CATTTTCCCGTCCTCATT
ALS1-R	CATTGCTATCTGGATTTGTG
ALS5-F	AGTTATGGGGATGTTGCT
ALS5-R	CTGGTTATTATCTGAGGG
HWP1-F	AGGTAGACGGTCAAGGTG
HWP1-R	TTGTGGCTGTTGTGGATA
INT1-F	CATTTTAGCCAACTCGTC
INT1-R	GAATCAGTTTCGTGCTCT
MNT1-F	GTCCGTGAACAAATGAGA
MNT1-R	GGTAAGGAAATAGTGAATGC
SAP2-F	CTACTCCAACAACAACCA
SAP2-R	CATCAGGAACCCATAAATC
PLB1-F	TATCTCAAGGTTTGTGGG
PLB1-R	ATAAGCATCACCTTCGTC
18S RNA-F	TCTTGTACGGCACATATCTCGT
18S RNA-R	GTTTCGAGAGGACCTTCAGTC

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转录因子Flo8 G723R、T751D突变增强白念珠菌毒力

Transcription factor Flo8 G723R and T751D mutations enhancing virulence in Candida albicans

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Metrics

菌株	基因型
SN152	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4
Flo8^SN152	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/ flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8-PAP
Flo8^G723R	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8G723R-PAP
Flo8^T751D	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/ flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8T751D-PAP