某院产ESBLs大肠埃希菌多位点序列分型研究

doi:10.3969/j.issn.1673-8640.2015.09.017

摘要/Abstract

摘要： 目的

了解郑州大学附属肿瘤医院临床和环境中产超广谱β-内酰胺酶(ESBLs)大肠埃希菌多位点序列分型(MLST)及其遗传进化关系,为监测产ESBLs菌株的流行和医院感染控制提供依据。

方法

采用基因扩增和测序的方法对26株产ESBLs大肠埃希菌7个管家基因进行序列分析,并与MLST数据库比对,得到每个菌株相应的等位基因号。

结果

26株产ESBLs大肠埃希菌获得16种序列分型(ST),其中3株为ST69型、3株为ST131型。

结论

产ESBLs大肠埃希菌遗传背景多样化,ST131型和ST69型为本研究主要型别,其它型别散在分布。

关键词: 超广谱β-内酰胺酶, 大肠埃希菌, 多位点序列分型

Abstract: Objective

To investigate the multilocus sequence typing (MLST) and genetic evolution of extended-spectrum beta-lactamase (ESBLs)-producing Escherichia coli in the Affiliated Cancer Hospital of Zhengzhou University, and to provide the reference for the monitoring of ESBLs-producing isolates' epidemic and hospital infection control.

Methods

Gene amplification and sequence analysis were performed for 7 housekeeping genes of 26 isolates of ESBLs-producing Escherichia coli, and alleles were compared with those assigned at MLST databases.

Results

A total of 16 sequence types (ST) were obtained in the 26 isolates of ESBLs-producing Escherichia coli, in which there were 3 ST69 and 3 ST131.

Conclusions

ESBLs-producing Escherichia coli has diversified genetic backgrounds, and ST131 and ST69 are the most prevalent types, while other types were scattered.

Key words: Extended-spectrum beta-lactamase, Escherichia coli, Multilocus sequence typing

中图分类号:

R378.2

潘军, 许青霞, 肖伟强, 常彦敏, 沈勇. 某院产ESBLs大肠埃希菌多位点序列分型研究[J]. 检验医学, 2015, 30(9): 939-943.

PAN Jun, XU Qingxia, XIAO Weiqiang, CHANG Yanmin, SHEN Yong. Multilocus sequence typing study of ESBLs-producing Escherichia coli in one hospital[J]. Laboratory Medicine, 2015, 30(9): 939-943.

图/表 5

参考文献 18

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管家基因	引物序列(5'-3')	长度(bp)	退火温度(℃)
adk	F:ATTCTGCTTGGCGCTCCGGG	583	54
	R:CCGTCAACTTTCGCGTATTT
fumC	F:TCACAGGTCGCCAGCGCTTC	806	54
	R:GTACGCAGCGAAAAAGATTC
gyrB	F:TCGGCGACACGGATGACGGC	911	60
	R:ATCAGGCCTTCACGCGCATC
icd	F:ATGGAAAGTAAAGTAGTTGTTCCGGCACA	878	54
	R:GGACGCAGCAGGATCTGTT
mdh	F:ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG	932	60
	R:TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT
purA	F:CGCGCTGATGAAAGAGATGA	816	54
	R:CATACGGTAAGCCACGCAGA
recA	F:CGCATTCGCTTTACCCTGACC	780	58
	R:TCGTCGAAATCTACGGACCGGA

管家基因	引物序列(5'-3')	长度(bp)	退火温度(℃)
adk	F:ATTCTGCTTGGCGCTCCGGG	583	54
	R:CCGTCAACTTTCGCGTATTT
fumC	F:TCACAGGTCGCCAGCGCTTC	806	54
	R:GTACGCAGCGAAAAAGATTC
gyrB	F:TCGGCGACACGGATGACGGC	911	60
	R:ATCAGGCCTTCACGCGCATC
icd	F:ATGGAAAGTAAAGTAGTTGTTCCGGCACA	878	54
	R:GGACGCAGCAGGATCTGTT
mdh	F:ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG	932	60
	R:TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT
purA	F:CGCGCTGATGAAAGAGATGA	816	54
	R:CATACGGTAAGCCACGCAGA
recA	F:CGCATTCGCTTTACCCTGACC	780	58
	R:TCGTCGAAATCTACGGACCGGA