MALDI-TOF MS在临床微生物样本直接检测中的应用

doi:10.3969/j.issn.1673-8640.2015.07.019

摘要/Abstract

摘要：

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)是近年来发展起来的一项新兴的微生物鉴定技术,与传统的生化表型鉴定方法和分子生物学方法相比,MALDI-TOF MS具有操作简单、快速、准确和经济的特点。鉴于其对培养出的纯菌落鉴定的准确性和稳定性,MALDI-TOF MS也可被应用于临床样本的直接检测,包括阳性血培养瓶、中段尿、脑脊液等。本文主要就MALDI-TOF MS 用于直接检测临床样本的原理、流程、鉴定效能及其研究进展等方面做一综述。

关键词: 基质辅助激光解吸电离飞行时间质谱, 直接检测, 微生物鉴定, 临床样本

Abstract:

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS),as a newly developed technology in recent years,is an easy,rapid,accurate and cost-effective method for microbial identification in clinical microbiology, compared to conventional phenotypic techniques or molecular biology. Given the accuracy and reliability of MALDI-TOF MS in the identification of microorganisms grown on solid medium,this technology might also be directly applied to some clinical microbiological samples, such as positive blood culture bottles,midstream urine, cerebrospinal fluid and so on. The goal of this review was to discuss the application of MALDI-TOF MS technology in direct identification of clinical microbiological samples, including the principles, protocols, performance and research progress.

Key words: Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Direct identification, Microbial identification, Clinical sample

中图分类号:

R446.5

陈飞, 胡玢婕, 赵虎. MALDI-TOF MS在临床微生物样本直接检测中的应用[J]. 检验医学, 2015, 30(7): 750-756.

CHEN Fei, HU Binjie, ZHAO Hu. Application of MALDI-TOF MS in direct identification of clinical microbiological samples[J]. Laboratory Medicine, 2015, 30(7): 750-756.

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