MALDI-TOF MS在临床微生物检验中的应用

doi:10.3969/j.issn.1673-8640.2015.02.002

摘要/Abstract

摘要：

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)是一种软电离质谱,适用于分析蛋白质等生物大分子物质,可用来绘制微生物的蛋白质图谱,达到鉴定的目的。基于MALDI-TOF MS的蛋白质谱技术具有快速、准确、敏感性高、高通量等特点,目前已应用于细菌和真菌的鉴定,其在微生物耐药性分析、分型和毒力研究等科研领域的应用也初有成效,尚有巨大的发展空间。本文就以MALDI-TOF MS为代表的蛋白质谱技术在微生物检验和科研中的应用作一综述。

关键词: 基质辅助激光解吸电离飞行时间质谱, 微生物, 检验, 科研

Abstract:

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is one kind of soft ionization mass spectrometry, which is suitable for the analysis of bio-macromolecule, such as protein. It can be used for microbial identification by mapping the microbial protein mass spectra. Since MALDI-TOF MS technology is well-known for its rapidity, accuracy, high sensitivity and high throughput, it has been applied in bacterium and fungus identifications. MALDI-TOF MS also has a good performance in the field of scientific research, including resistance analysis, bacterium typing and virulence study,and there is still a large space for development. In this paper, we reviewed the application of MALDI-TOF MS technology in microbial identification and scientific research.

Key words: Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Microbiology, Determination, Scientific research

中图分类号:

R446.5

戴颖欣, 李敏. MALDI-TOF MS在临床微生物检验中的应用[J]. 检验医学, 2015, 30(2): 102-107.

DAI Yingxin, LI Min. Application of MALDI-TOF MS technology in clinical microbial determination[J]. Laboratory Medicine, 2015, 30(2): 102-107.

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