检验医学 ›› 2015, Vol. 30 ›› Issue (2): 173-176.DOI: 10.3969/j.issn.1673-8640.2015.02.017

• 技术研究与评价·论著 • 上一篇    下一篇

蛋白质指纹图谱技术在诊断白血病四种分型中的应用

黄华, 陈林兴, 林美册, 黄靖宇   

  1. 汕头大学医学院第二附属医院,广东 汕头 515041
  • 收稿日期:2014-08-14 出版日期:2015-02-28 发布日期:2015-02-12
  • 作者简介:null

    作者简介:黄华,女,1975年生,本科,副主任技师,主要从事骨髓细胞形态学工作。

  • 基金资助:
    广东省科技计划项目(2010B031600272)

Application of protein fingerprinting technology in diagnosing 4 types of leukemia

HUANG Hua, CHEN Linxing, LIN Meice, HUANG Jingyu.   

  1. The Second Hospital Affiliated to Shantou University School of Medicine, Guangdong Shantou 515041,China
  • Received:2014-08-14 Online:2015-02-28 Published:2015-02-12

摘要: 目的

对白血病典型的4种分型血清样本进行检测分析,建立白血病分型的蛋白质指纹图谱诊断模型。

方法

应用蛋白质指纹图谱技术检测16例急性粒细胞白血病M2a、18例慢性粒细胞白血病、14例急性淋巴细胞白血病及16例急性粒细胞白血病M3,采用Biomarker Wizard 及Biomarker Patterns 5.0软件进行标记物的比较判别,建立决策树诊断模型。

结果

由m/z 3 376、8 055及9 421建立决策树诊断模型,其中16例急性粒细胞白血病M2a检出率为75.0%(12/16)、9例慢性粒细胞白血病检出率为100.0%(18/18)、14例急性淋巴细胞白血病检出率71.4%(10/14)、16例急性粒细胞白血病M3检出率为87.5%(12/14)。

结论

由3个蛋白标记峰构成的蛋白质指纹图谱诊断模型为白血病4种分型的诊断提供了新的借鉴和参考。

关键词: 白血病, 分型, 蛋白质指纹图谱技术

Abstract: Objective

To establish protein fingerprinting model for the diagnosis of leukemia through determining 4 typical types of leukemia.

Methods

A total of 16 acute myeloblastic leukemia M2a samples, 18 chronic myelocytic leukemia samples, 14 acute lymphoblastic leukemia samples and 16 acute myeloblastic leukemia M3 samples were determined by protein fingerprinting technology. The comparison of biomarkers was performed by Biomarker Wizard and Biomarker Patterns 5.0 to establish a diagnosis tree model.

Results

The diagnosis tree model combined with 3 biomarkers ( m/z: 3 376, 8 055 and 9 421) could detect 75% acute myeloblastic leukemia M2a (12/16),100.0% chronic myelocytic leukemia (18/18), 71.4% acute lymphoblastic leukemia (10/14) and 87.5% acute myeloblastic leukemia M3 (12/14).

Conclusions

The protein fingerprinting model established by 3 protein features maybe a novel method for diagnosing 4 types of leukemia.

Key words: Leukemia, Typing, Protein fingerprinting technology

中图分类号: