五味子对鲍曼不动杆菌体外杀菌和抑菌作用的研究

doi:10.3969/j.issn.1673-8640.2014.06.020

检验医学 ›› 2014, Vol. 29 ›› Issue (6): 664-667.DOI: 10.3969/j.issn.1673-8640.2014.06.020

五味子对鲍曼不动杆菌体外杀菌和抑菌作用的研究

张景皓¹,李艳红²,赵虎¹

1. 上海复旦大学附属华东医院检验科,上海 200040;
2. 新疆医科大学第一附属医院医学检验中心,新疆乌鲁木齐 830054

收稿日期:2014-03-17 出版日期:2014-06-30 发布日期:2014-06-23
通讯作者: 赵虎,联系电话:021-62483180-20847。
作者简介:张景皓,女,1988年生,学士,主要从事临床微生物学研究。

Study on bactericidal and bacteriostatic effects of Schisandra chinensis fruit against Acinetobacter baumannii in vitro

ZHANG Jinghao¹, LI Yanhong², ZHAO Hu¹

1. Department of Clinical Laboratory, Huadong Hospital, Fudan University, Shanghai 200040, China;
2. Center for Clinical Laboratory, the First Hospital Affiliated to Xinjiang Medical University, Xinjiang Urumqi 830054, China

Received:2014-03-17 Online:2014-06-30 Published:2014-06-23

摘要/Abstract

摘要： 目的研究中药五味子对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌的杀菌和抑菌作用。方法制备五味子浸液,采用等倍稀释法进行体外抑菌和杀菌试验,分别测定五味子药液对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果五味子对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌的MIC50分别为15.6和7.8 mg/mL,在此药物浓度处,对头孢哌酮-舒巴坦非耐药的鲍曼不动杆菌的抑菌率均高于耐药的菌株(χ²=4.26、3.98,P均<0.05);当药物浓度达到31.3 mg/mL时对二者的抑菌率均为100%;五味子对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌的MBC50均为31.3 mg/mL,杀菌率差异无统计学意义(χ²=0.74, P>0.05)。结论五味子在体外对头孢哌酮-舒巴坦耐药和非耐药的鲍曼不动杆菌均有一定的杀菌和抑菌作用,对头孢哌酮-舒巴坦耐药的菌株MIC略高,可尝试用于临床鲍曼不动杆菌感染的治疗。

关键词: 五味子, 鲍曼不动杆菌, 头孢哌酮-舒巴坦

Abstract: Objective To observe the bactericidal and bacteriostatic effects of Schisandra chinensis fruit against Acinetobacter baumannii which was resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam. Methods The extract of Schisandra chinensis fruit was prepared, bacteriostatic and bactericidal test in vitro by tube double dilution method was performed to observe their minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) to Acinetobacter baumannii. Results The MIC50 of Schisandra chinensis fruit against Acinetobacter baumannii resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam were 15.6 and 7.8 mg/mL, and on this drug concentration, the inhibitory rate against non-resistant to cefoperazone-sulbactam Acinetobacter baumannii was higher than that against resistant to cefoperazone-sulbactam (χ²=4.26 and 3.98, P<0.05). When the drug concentration reached to 31.3mg/mL, the inhibitory rates were all 100%. The MBC50 of Schisandra chinensis fruit against Acinetobacter baumannii which was resistant tocefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam were 31.3 mg/mL, and the bactericidal rate had no statistical significance (χ²=0.74, P>0.05). Conclusions The Schisandra chinensis fruit has certain bactericidal and bacteriostatic effects on Acinetobacter baumannii which was resistant to cefoperazone-sulbactam or non-resistant to cefoperazone-sulbactam in vitro, and the MIC against Acinetobacter baumannii is a little higher. It suggests that Schisandra chinensis fruit could be used for the clinical treatment of Acinetobacter baumannii.

Key words: Schisandra chinensis fruit, Acinetobacter baumannii, Cefoperazone-sulbactam

中图分类号:

R446.5

张景皓,李艳红,赵虎. 五味子对鲍曼不动杆菌体外杀菌和抑菌作用的研究[J]. 检验医学, 2014, 29(6): 664-667.

ZHANG Jinghao, LI Yanhong, ZHAO Hu. Study on bactericidal and bacteriostatic effects of Schisandra chinensis fruit against Acinetobacter baumannii in vitro[J]. , 2014, 29(6): 664-667.

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五味子对鲍曼不动杆菌体外杀菌和抑菌作用的研究

Study on bactericidal and bacteriostatic effects of Schisandra chinensis fruit against Acinetobacter baumannii in vitro

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