血清ox-LDL与非酒精性脂肪肝超声检查关系的探讨

doi:10.3969/j.issn.1673-8640.2014.02.007

检验医学 ›› 2014, Vol. 29 ›› Issue (2): 118-121.DOI: 10.3969/j.issn.1673-8640.2014.02.007

血清ox-LDL与非酒精性脂肪肝超声检查关系的探讨

胡可胜¹, 马艳华¹, 刘连红¹, 李伟², 罗建祥¹

1. 武警广东总队医院检验科, 广东广州 510000;
2. 武警广东总队医院消化科, 广东广州 510000

收稿日期:2013-01-08 出版日期:2014-02-20 发布日期:2014-02-20
作者简介:胡可胜, 男, 1984年生, 硕士, 技师, 主要从事代谢性疾病的诊断、机制研究。

Investigation on the correlation of serum ox-LDL level with ultrasound image for nonalcoholic fatty liver disease

HU Kesheng¹, MA Yanhua¹, LIU Lianhong¹, LI Wei², LUO Jianxiang¹

1. Department of Clinical Laboratory, Guangdong Province Hospital of the People′s Arms Corps of China, Guangdong Guangzhou 510000, China; 2. Department of Gastroenterology, Guangdong Province Hospital of the People′s Arms Corps of China, Guangdong Guangzhou 510000, China

Received:2013-01-08 Online:2014-02-20 Published:2014-02-20

摘要/Abstract

摘要：

目的探讨不同程度非酒精性脂肪肝(NAFLD)患者血清氧化低密度脂蛋白(ox-LDL)水平及其与代谢指标的相关性。方法采用酶联免疫吸附试验(ELISA)检测79例NAFLD患者和23名正常对照者血清ox-LDL浓度, 同时测定肝功能、代谢指标及血清同型半胱氨酸(Hcy)水平, 根据B超检查结果将79例NAFLD患者分为轻(40例)、中(25例)、重度(14例)脂肪肝。分析ox-LDL与其他指标及脂肪肝程度的相关性。结果 NAFLD组丙氨酸氨基转移酶、γ谷氨酰基转移酶、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、载脂蛋白B、载脂蛋白E、Hcy、尿酸、空腹血糖、纤维连接蛋白及ox-LDL水平均明显高于正常对照组(P均＜0.05)。中、重度脂肪肝组血清ox-LDL水平明显高于轻度脂肪肝组(P均＜0.05)。ox-LDL与Hcy、尿酸、总胆汁酸呈正相关关系[相关系数(r)为0.375、0.369和0.395, P值分别为0.007、0.013和0.029], ox-LDL水平与脂肪肝的分级呈正相关(r＝0.410, P＝0.011)。结论随着脂肪肝程度的加重, NAFLD患者血清ox-LDL水平逐渐升高。ox-LDL是肝功能受损及脂肪肝进展过程中的重要因素, 对临床诊断和病情监测有重要意义。

关键词: 氧化低密度脂蛋白, 脂肪肝程度, 同型半胱氨酸非, 酒精性脂肪肝

Abstract:

Objective To investigate the correlation of serum oxidized-low density lipoprotein (ox-LDL) with metabolism parameters in different graded nonalcoholic fatty liver disease(NAFLD) patients. Methods Enzyme-linked immunosorbert assay (ELISA) was used to determine the levels of serum ox-LDL of 79 NAFLD patients and 23 healthy controls, and the liver functions, metabolism parameters and serum homocysteine (Hcy) were determined simultaneously. By ultrasound image as the diagnostic standard, NAFLD patients were classified into mild (40 cases), moderate (25 cases) and severe (14 cases) fatty liver groups. The correlation of ox-LDL with other parameters and fatty liver infiltration grade was analyzed. Results Alanine aminotransferase, gamma-glutamyltransferase, triglyceride, total cholesterol, low-density lipoprotein cholesterol, apolipoprotein B, apolipoprotein E, Hcy, uric acid, fasting blood glucose, fibronectin and ox-LDL were significantly higher in NAFLD group than in healthy control group(P<0.05). Serum ox-LDL levels in mild fatty liver group were significantly lower than those in moderate fatty liver group and severe fatty liver group(P<0.05). Serum ox-LDL was positively correlated with Hcy, uric acid and total bile acid levels [correlation coefficients (r)=0.375, 0.369 and 0.395; P=0.007, 0.013 and 0.029], and was positively correlated with fatty liver infiltration grade (r＝0.410, P＝0.011). Conclusions The serum ox-LDL level in NAFLD patients increases with the increasing of fatty liver infiltration grade, and ox-LDL is an important factor in liver function impairment and fatty liver disease progression.

Key words: Oxidized-low density lipoprotein, Fatty Liver infiltration grade, Homocysteine, Nonalcoholic fatty liver disease

中图分类号:

R446.1

胡可胜, 马艳华, 刘连红, 李伟, 罗建祥. 血清ox-LDL与非酒精性脂肪肝超声检查关系的探讨[J]. 检验医学, 2014, 29(2): 118-121.

HU Kesheng, MA Yanhua, LIU Lianhong, LI Wei, LUO Jianxiang. Investigation on the correlation of serum ox-LDL level with ultrasound image for nonalcoholic fatty liver disease[J]. , 2014, 29(2): 118-121.

参考文献

[1] Wang CC, Tseng TC, Hsieh TC, et al. Severity of fatty liver on ultrasound correlates with metabolic and cardiovascular risk[J]. Kaohsiung J Med Sci, 2012, 28(3):151-160.
[2] 王立, 何苗苗, 周宇谦. 超声定量分析脂肪肝图像与肝功能的关系[J]. 中国临床医学, 2005, 12(5):904-907.
[3] Smith BW, Adams LA. Nonalcoholic fatty liver disease and diabetes mellitus: pathogenesis and treatment[J]. Nat Rev Endocrinol, 2011, 7(8):456-465.
[4] 李洁, 王翎, 谢燕, 等. 老年阻塞性睡眠呼吸暂停低通气综合征患者血清同型半胱氨酸水平及其机制研究[J]. 中华老年医学杂志, 2011, 30(7):558-561.
[5] 范建高, 郑晓英, 张梅, 等.非酒精性脂肪性肝病大鼠肝窦超微结构及微血栓观察[J].肝脏, 2005, 10(1):41-42.
[6] Chiang CH, Huang PH, Chung FP, et al. Decreased circulating endothelial progenitor cell levels and function in patients with nonalcoholic fatty liver disease[J]. PLoS One, 2012, 7(2): e31799.
[7] Polyzos SA, Kountouras J, Patsiaoura K, et al. Serum homocysteine levels in patients with nonalcoholic fatty liver disease [J]. Ann Hepatol, 2012, 11(1):68-76.
[8] Fu CC, Chen MC, Li YM, et al. The risk factors for ultrasound-diagnosed non-alcoholic fatty liver disease among adolescents[J]. Ann Acad Med Singapore, 2009, 38(1):15-17.
[9] 周艺峰.乙肝患者纤维连接蛋白的检测与意义[J]. 临床消化病杂志, 1996, 8(3):126.
[10] 龚显恩, 刘娟.肝硬化合并肝癌患者血清甲胎蛋白、胆碱酯酶、总胆汁酸水平与Child-Pugh分级间的相互关系[J]. 检验医学, 2012, 27(1):79-80.
[11] 董春富, 吴国华. 脂肪肝患者血脂、肝功能指标改变的分析[J]. 检验医学, 2005, 20(2): 160-161.
[12] Olorunnisola OS, Bradley G, Afolayan AJ. Protective effect of T. violacea rhizome extract against hypercholesterolemia-induced oxidative stress in Wistar rats[J]. Molecules, 2012, 17(5):6033-6045.
[13] Boushey CJ, Beresford SA, Omenn GS, et al. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes[J]. JAMA, 1995, 274(13):1049-1057.
[14] 杨慧君, 李倩, 崔玲, 等. 非酒精性脂肪肝患者同型半胱氨酸浓度及叶酸与维生素B12浓度变化及相关性[J]. 世界华人消化杂志, 2007, 15(12):1449-1452.
[15] 陈小莉, 杨庆, 蔡东联, 等. 同型半胱氨酸抑制主动脉内皮细胞生长的研究[J]. 第二军医大学学报, 2002, 23(2):196-199.
[16] 刘久波, 何国厚, 杨青山, 等. Ox-LDL与血液流变性的关系及其对脑梗塞的影响[J]. 中国微循环, 2001, 5(3):223-225.
[17] 王瑜, 蔡文仙, 牛峰海, 等. 血清同型半胱氨酸与肥胖儿童氧化低密度脂蛋白、丙二醛及体重指数的相关性[J]. 中华妇幼临床医学杂志(电子版), 2010, 6(1):96-99.

血清ox-LDL与非酒精性脂肪肝超声检查关系的探讨

Investigation on the correlation of serum ox-LDL level with ultrasound image for nonalcoholic fatty liver disease

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

[1]	王永斌, 陆奎英, 苏勇, 张俊, 刘付弟. 非酒精性脂肪肝患者肝功能、氧化应激水平变化及临床意义[J]. 检验医学, 2020, 35(9): 903-907.
[2]	刘军锋, 敬锐, 刘雅宁, 霍茜瑜, 刘志礼, 贾克刚, 李永姝, 韩雪晶, 林文华, 刘运德. 单核细胞LOX-1、CX3CR1表达与冠状动脉近段/中段狭窄的关系[J]. 检验医学, 2017, 32(11): 1024-1030.
[3]	周琳, 蒋理, 范宇鹏, 陆继强, 朱燕忠, 沈振海. 非酒精性脂肪肝与血清CK-18、肝纤维化指标的相关性分析[J]. 检验医学, 2016, 31(2): 99-102.
[4]	江帆, 陈小庄, 周晓莉, 仝珊. 晚期糖基化终末产物、氧化低密度脂蛋白与老年糖尿病合并骨质疏松症的关系[J]. 检验医学, 2016, 31(10): 883-888.
[5]	何文军, 黄家平, 梁伟东, 莫翔, 梁少华. 氧化低密度脂蛋白和C反应蛋白与冠状动脉支架置入术后再狭窄的关系研究[J]. 检验医学, 2015, 30(5): 484-488.
[6]	牛冬梅, 洪骏, 吴嘉, 宋佳希, 于瑞杰, 袁云龙, 汪俊军. 儿童肾病综合征患者血清视黄醇结合蛋白4水平与氧化低密度脂蛋白的关系研究[J]. 检验医学, 2014, 29(2): 122-129.
[7]	张亮;陈志强;文剑. 脂联素影响体外培养肝细胞乙醛氧化酶1表达的研究[J]. 检验医学, 2012, 27(4): 275-279.
[8]	李新征;陈宝炳;康云平. 非酒精性脂肪肝患者血清游离脂肪酸与胰岛素抵抗的关系[J]. 检验医学, 2012, 27(1): 48-49.
[9]	李小洲;朱波;王新敏;杨延龙;陈巍巍. 外周血中血凝素样氧化低密度脂蛋白受体蛋白表达与冠心病相关性研究[J]. 检验医学, 2008, 23(05): 450-453.
[10]	樊锦秀;朱慧民;金国健;陈琪;张黎明;王爱华;赖卫强. 老年代谢综合征与非酒精性脂肪肝患者载脂蛋白E基因多态性检测的意义[J]. 检验医学, 2006, 21(06): 637-641.
[11]	潘逸茹;薛惠君;龚立琪. 胰岛素样生长因子-Ⅰ在非酒精性脂肪肝病变中的变化[J]. 检验医学, 2005, 20(04): 300-302.