大黄对重症急性胰腺炎患者血清IL-10、IL-18影响的研究

doi:10.3969/j.issn.1673-8640.2013.07.005

检验医学 ›› 2013, Vol. 28 ›› Issue (7): 581-584.DOI: 10.3969/j.issn.1673-8640.2013.07.005

大黄对重症急性胰腺炎患者血清IL-10、IL-18影响的研究

余秀文^¹, 吴素芬^²

1.黄石市第五医院检验科,湖北黄石 435005;
2. 黄石市第五医院消化内科实验室,湖北黄石435005

收稿日期:2013-07-29 修回日期:2013-07-29 出版日期:2013-07-25 发布日期:2013-07-29
通讯作者: 吴素芬,联系电话:0714-5329022
作者简介:余秀文,女,1976年生,主管技师,主要从事临床化学检验工作。

Research on the influence of rude rhubarb on serum IL-10 and IL-18 levels in patients with severe acute pancreatitis

YU Xiuwen^¹,WU Sufen^²

1.Department of Clinical Laboratory,the Fifth Hospital of Huangshi, Hubei Huangshi 435005,China;
2.Department of Nephrology, the Fifth Hospital of Huangshi, Hubei Huangshi 435005, China

Received:2013-07-29 Revised:2013-07-29 Online:2013-07-25 Published:2013-07-29

摘要/Abstract

摘要： 目的探讨大黄对重症急性胰腺炎(SAP)患者血清白细胞介素10(IL-10)、白细胞介素18(IL-18)的影响。方法 58例SAP患者随机分为A组(22例)、B组(36例)。A组只行综合治疗,B组在综合治疗基础上给予鼻空肠管注入大黄。采用酶联免疫吸附试验(ELISA)检测患者入院时 (0 d)、入院后7和14 d时血清IL-10、IL-18水平。以健康体检人群血清作为对照。结果入院(0 d)时A、B组IL-18水平均显著高于对照组(P<0.01),IL-10水平也显著高于对照组(P<0.05),但IL-18、IL-10在A、B组之间差异无统计学意义(P>0.05)。治疗7 d时A、B组IL-18水平均较入院时下降,其中以B组下降最明显;A、B组之间IL-18水平差异有统计学意义(P<0.01);治疗7 d时A组IL-10水平与入院时无差异,但B组IL-10水平明显升高(P<0.01)。治疗14 d时A、B组IL-18水平均分别较治疗7 d时下降,但差异无统计学意义(P>0.05),而A、B组之间IL-18水平差异有统计学意义(P<0.01);治疗14 d时A、B组IL-10水平均分别较治疗7 d时升高,但差异无统计学意义(P>0.05),而A、B组之间IL-10水平差异仍有统计学意义(P<0.05)。在综合治疗基础上给予大黄治疗后,B组总有效率均明显高于A组(P<0.05)。相关分析显示,A、B组血清IL-18与血淀粉酶呈正相关(r_A=0.55,P<0.05;r_B=0.81,P<0.01),与尿淀粉酶也呈正相关(r_A=0.51,P<0.05;r_B=0.79,P<0.01);A、B组血清IL-10与血淀粉酶呈负相关(r_A=-0.46,P<0.05;r_B=-0.72,P<0.01),与尿淀粉酶也呈负相关(r_A=-0.44,P<0.05;r_B=-0.63,P<0.01)。结论通过调节炎症介质IL-10、IL-18的表达,大黄能有效地逆转SAP病情。

关键词: 白细胞介素10, 白细胞介素18, 大黄, 重症急性胰腺炎, 鼻空肠管

Abstract: Objective To research the influence of rude rhubarb on serum interleukin 10 (IL-10) and interleukin 18 (IL-18) levels in patients with severe acute pancreatitis (SAP). Methods SAP patients were classified into group A(22 patients) and group B (36 patients) randomly.The patients in group A were given comprehensive treatment.On the basis of comprehensive treatment,the patients in group B were given rude rhubarb by nasojejunal feeding. The serum levels of IL-10 and IL-18 in the 2 groups were detected by enzyme-linked immunosorbert assay (ELISA) at different intervals (0 d, 7 d and 14 d). The sera of healthy subjects were as controls. Results On 0 d, the serum levels of IL-18 in A and B groups were higher than those in the control group (P<0.01), and the serum levels of IL-10 in A and B groups were also higher than those in the control group (P<0.05). However, the serum levels of IL-18 and IL-10 between A and B groups had no statistical significance (P>0.05). On 7 d,the serum levels of IL-18 in A and B groups decreased compared with those on 0 d, especially in group B, and there were statistical significances between A and B groups (P<0.01). On 7 d,the serum levels of IL-10 in A group didn′t change obviously compared with those on 0 d, but the serum levels of IL-10 in B group significantly increased compared with those on 0 d (P<0.01) .On 14 d, the serum levels of IL-18 in A and B groups decreased compared with those on 7 d with no statistical significance (P>0.05), but the levels of IL-18 between A and B groups showed significant differences(P<0.01). On 14 d, the serum levels of IL-10 in A and B groups increased compared with those on 7 d with no statistical significance (P>0.05), but the levels of IL-10 between A and B groups showed significant differences (P<0.05). The total effective rate in B group after administration of rude rhubarb was higher than that in A group (P<0.05). IL-18 and blood amylases were positively correlated (r_A=0.55,P<0.05;r_B=0.81,P<0.01), and IL-18 and urine amylases were positively correlated (r_A=0.51,P<0.05;r_B=0.79,P<0.01). There was negative correlation between IL-10 and blood amylases(r_A=-0.46,P<0.05;r_B=-0.72,P<0.01), and urine amylases(r_A=-0.44,P<0.05;r_B=-0.63,P<0.01)in A and B groups. Conclusions The administration of rude rhubarb can effectively reverse SAP by regulating the serum levels of IL-10 and IL-18.

Key words: Interleukin 10, Interleukin 8, Rude rhubarb, Severe acute pancreatitis, Nasojejunal tube

中图分类号:

R446.62

余秀文, 吴素芬. 大黄对重症急性胰腺炎患者血清IL-10、IL-18影响的研究[J]. 检验医学, 2013, 28(7): 581-584.

YU Xiuwen,WU Sufen. Research on the influence of rude rhubarb on serum IL-10 and IL-18 levels in patients with severe acute pancreatitis[J]. , 2013, 28(7): 581-584.

参考文献

[1] Hong W, Dong L, Huang Q, et al.Prediction of severe acute pancreatitis using classification and regression tree analysis[J].Dig Dis Sci, 2011,56(12):3664-3671.

[2] Cruz-Santamaría DM, Taxonera C, Giner M.Update on pathogenesis and clinical management of acute pancreatitis[J].World J Gastrointest Pathophysiol, 2012,3(3):60-70.

[3] Umezawa M, Tanaka N, Takeda K, et al. Clari-thromycin and telithromycin increases interleukin-10 expression in the rat endometriosis model[J].Cytokine, 2011, 55(3):339-342.

[4] 姚圣华,朱阳春,李淑德.清胰汤及生大黄灌肠治疗重症急性胰腺炎最佳时间探讨[J].中华胰腺病杂志,2008,8(5):324-325.

[5] 吴培俊,盛爱华.复方大黄对胰腺炎患者胰管压力的影响[J].胰腺病学,2003,3(2):105-122.

[6] 中华医学会消化病学分会胰腺病学组.重症急性胰腺炎内科规范治疗建议[J].中华消化杂志, 2009,29(2):75-78.

[7] Gregoric P, Sijacki A, Stankovic S, et al. SIRS score on admission and initial concentration of IL-6 as severe acute pancreatitis outcome predictors[J]. Hepatogastroenterology, 2010, 57(98): 349-353.

[8] Pooran N,Indaram A,Singh P,et al.Cytokines(IL-6,IL-8,TNF):early and reliabe predictors of severe acute pancreatitis[J]. J Clin Gastroenterol, 2003, 37(3): 263-266.

[9] Leung BP, Culshaw S, Gracie JA, et al. A role for IL-18 in neutrophil activation[J]. J Immunol, 2001, 167(5): 2879-2886.

[10] Van de Veerdonk FL, Netea MG, Dinarello CA, et al.Inflammasome activation and IL-1β and IL-18 processing during infection[J].Trends Immunol, 2011, 32(3): 110-116.

[11] Culshaw S, Leung BP, Gracie JA, et al.Prior elevation of IL-18 promotes rapid early IFN-gamma production during staphylococcal infection[J]. Eur J Immunol, 2005, 35(5): 1438-1444.

[12] Rau B, Baumgart K, Paszkowski AS, et al. Clinical relevance of caspase-1 activated cytokines in acute pancreatitis: high correlation of serum interleukin-18 with pancreatic necrosis and systemic complications[J]. Crit Care Med, 2001, 29(8): 1556-1562.

[13] Wereszczynska-Siemiatkowska U, Mroczko B, Siemiatkowski A. Serum profiles of interleukin-18 in different severity forms of human acute pancreatitis[J]. Scand J Gastroenterol, 2002, 37(9): 1097-1102.

[14] Mayerle J, Dummer A, Sendler M, et al. Differential roles of inflammatory cells in pancreatitis[J]. J Gastroenterol Hepatol, 2012, 27(Suppl 2): 47-51.

[15] Wereszczynska-Siemiatkowska U, Dabrowski A, Siemiatkowski A, et al. Serum profiles of E-selectin,interleukin-10, and interleukin-6 and oxidative stress parameters in patients with acute pancreatitis and nonpancreatic acute abdominal pain[J]. Pancreas, 2003, 26(2): 144-152.

[16] 刘晓红,赵雩卿,钱家鸣.大黄对大鼠急性出血性胰腺炎的影响[J].中华消化杂志,2004,24(1):14-17.

大黄对重症急性胰腺炎患者血清IL-10、IL-18影响的研究

Research on the influence of rude rhubarb on serum IL-10 and IL-18 levels in patients with severe acute pancreatitis

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

[1]	丁肖媛, 戴劲盛, 焦荣红, 马素丽, 朱海峰, 吴梦雅, 车艳然, 张蕾. 肥胖/超重对哮喘发作期患儿Th1/Th2和Th17/Treg细胞因子的影响[J]. 检验医学, 2023, 38(8): 748-752.
[2]	任荟蓉, 徐婷娟, 武涛, 黄玫, 杨亮. 血清颗粒蛋白前体在Ⅰ型自身免疫性肝炎预后评估中的价值[J]. 检验医学, 2023, 38(3): 245-250.
[3]	吴苏君, 冀恒涛, 彭梦乐. IL-18、IL-37、NLRP3及NK/DC比值在乙型肝炎相关肝硬化中的临床价值[J]. 检验医学, 2022, 37(6): 518-523.
[4]	陈朴, 马艳婷, 陈楠, 于正麟, 程韵枫, 潘柏申, 郭玮, 王蓓丽. 炎症相关细胞因子与Ph阴性骨髓增殖性肿瘤的相关性[J]. 检验医学, 2021, 36(2): 167-172.
[5]	洪伟祥, 宋云霄, 贾玖德, 张文通, 沈文晖, 黄晋旺, 张雷, 米尔萨力江·亚森, 张银网. 术前血清IL-2R、IL-6和IL-8水平与老年髋关节骨折术后30 d预后的相关性[J]. 检验医学, 2021, 36(11): 1159-1163.
[6]	林瀚, 宋颖, 蔡祺, 郭平, 陈骊婷. 平均中性粒细胞体积在重症急性胰腺炎合并菌血症中的应用[J]. 检验医学, 2020, 35(7): 660-662.
[7]	姜文佳. 骨关节炎患者血清1, 25（OH） ₂D₃、IL-17和IL-10水平的变化及临床意义[J]. 检验医学, 2019, 34(3): 202-205.
[8]	陆光辉, 褚志华, 周发为, 杨年安, 杨洁, 王姣平, 曾守逵. IL-17、IL-22和IL-10在支气管哮喘患儿外周血单个核细胞中的表达及意义[J]. 检验医学, 2019, 34(1): 28-32.
[9]	张国英, 钮晓红, 徐卫平, 夏厦. 淋巴结核患者外周血CD4⁺CD25^highFoxP3⁺调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义[J]. 检验医学, 2015, 30(1): 31-35.
[10]	陈永琴，金文君，戴梦璐. 慢性乙型肝炎患者IFN-γ、IL-10和CD19＋水平检测与病毒载量的关系[J]. 检验医学, 2013, 28(4): 315-317.
[11]	蒯守刚，戴亚萍，何琳静，王旭，刘君，刘斌，裴豪. IL-17、IL-1β和IL-10在结核病患者中的表达及临床意义[J]. 检验医学, 2012, 27(11): 973-974.
[12]	金磊;倪培华;吴洁敏;傅毅;葛海良. 白细胞介素10-819C/T、-1082G/A基因多态性与脑梗死的关系[J]. 检验医学, 2011, 26(11): 717-721.
[13]	金磊;倪培华;吴洁敏;傅毅;葛海良. 白细胞介素10-819C/T、-1082G/A基因多态性与脑梗死的关系[J]. 检验医学, 2011, 26(11): 717-721.
[14]	王丽芳;李永军;张晋霞;郝冀洪. 血清新喋呤检测在重症急性胰腺炎患者中的价值[J]. 检验医学, 2007, 22(01): 1-3.