淋巴结核患者外周血CD4+CD25highFoxP3+调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义

doi:10.3969/j.issn.1673-8640.2015.01.008

检验医学 ›› 2015, Vol. 30 ›› Issue (1): 31-35.DOI: 10.3969/j.issn.1673-8640.2015.01.008

淋巴结核患者外周血CD4⁺CD25^highFoxP3⁺调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义

张国英, 钮晓红, 徐卫平, 夏厦

南京中医药大学附属南京市中西医结合医院检验科,江苏南京 210014

收稿日期:2014-01-21 出版日期:2015-01-30 发布日期:2015-02-02
作者简介:null
作者简介:张国英,女,1978年生,硕士,副主任技师,主要从事微生物与免疫学研究。
基金资助:
南京市医学科技发展项目(QYK11146)

Levels of CD4⁺CD25^highFoxP3⁺ regulatory T lymphocytes in peripheral blood and plasma IFN-γ, IL-10 of patients with lymph tuberculosis and their clinical significance

ZHANG Guoying, NIU Xiaohong, XU Weiping, XIA Xia.

Department of Clinical Laboratory, Nanjing Hospital of Integrated Traditional and Western Medicine, Nanjing University of Traditional Chinese Medicine, Jiangsu Nanjing 210014, China

Received:2014-01-21 Online:2015-01-30 Published:2015-02-02

摘要/Abstract

摘要：

目的研究淋巴结核患者外周血CD4⁺CD25^highFoxP3⁺调节性T淋巴细胞(Treg)和干扰素γ(IFN-γ)、白细胞介素10(IL-10)水平的变化及临床意义。方法采用流式细胞仪对100例淋巴结核患者及30名正常人外周血CD4⁺CD25^highFoxP3⁺Treg和IFN-γ、IL-10水平进行检测。结果淋巴结核患者组与正常对照组比较,外周血CD4⁺CD25^highFoxP3⁺Treg和IL-10水平均升高(P<0.05),而IFN-γ水平则降低(P<0.05)。在干酪样型、增殖型和混合型3种类型的淋巴结核病之间各指标差异均无统计学意义(P>0.05)。结论淋巴结核患者细胞免疫功能明显异常。CD4⁺CD25^highFoxP3⁺Treg和IFN-γ、IL-10在淋巴结核的发病过程中发挥了重要作用。

关键词: CD4+CD25highFoxP3+调节性T淋巴细胞, 干扰素γ, 白细胞介素10, 淋巴结核

Abstract:

Objective To study the changes of peripheral blood CD4⁺CD25^highFoxP3⁺ regulatory T lymphocytes (Treg) and interferon gamma (IFN-γ), interleukin 10 (IL-10) levels of patients with lymph tuberculosis and their clinical significance. Methods The levels of peripheral blood CD4⁺CD25^highFoxP3⁺ Treg, IFN- γ and IL-10 of 100 lymph tuberculosis patients and 30 healthy subjects were determined by flow cytometry. Results Compared with the healthy controls, the levels of peripheral blood CD4⁺CD25^highFoxP3⁺ Treg and IL-10 levels in lymph tuberculosis group increased with statistical significance (P<0.05), while IFN-γ level decreased (P<0.05). Among the caseous, proliferation and mixed types of lymph tuberculosis, there was no significant difference (P>0.05). Conclusions Cellular immune function is significantly abnormal in patients with lymph tuberculosis. CD4⁺CD25^highFoxP3⁺ Treg, IFN- γ and IL-10 play important roles in the onset process of lymph tuberculosis.

Key words: CD4+CD25highFoxP3+ regulatory T lymphocyte, Interferon gamma, Interleukin 10, Lymph tuberculosis

中图分类号:

R446.63

张国英, 钮晓红, 徐卫平, 夏厦. 淋巴结核患者外周血CD4⁺CD25^highFoxP3⁺调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义[J]. 检验医学, 2015, 30(1): 31-35.

ZHANG Guoying, NIU Xiaohong, XU Weiping, XIA Xia.. Levels of CD4⁺CD25^highFoxP3⁺ regulatory T lymphocytes in peripheral blood and plasma IFN-γ, IL-10 of patients with lymph tuberculosis and their clinical significance[J]. Laboratory Medicine, 2015, 30(1): 31-35.

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参考文献 15

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组别	例数	CD4⁺CD25^highFoxP3⁺Treg(%)	IFN-γ(pg/mL)	IL-10(pg/mL)
淋巴结核组	100	2.32±0.29	16.62±1.63	16.74±2.21
正常对照组	30	2.01±0.49	19.07±2.85	14.50±1.56

组别	例数	CD4⁺CD25^highFoxP3⁺Treg(%)	IFN-γ(pg/mL)	IL-10(pg/mL)
淋巴结核组	100	2.32±0.29	16.62±1.63	16.74±2.21
正常对照组	30	2.01±0.49	19.07±2.85	14.50±1.56

组别	例数	CD4⁺CD25^highFoxP3⁺Treg(%)	IFN-γ(pg/mL)	IL-10(pg/mL)
干酪样型组	21	2.29±0.52	16.28±0.75	17.05±2.13
增殖型组	32	2.36±0.14	17.19±3.35	16.01±2.45
混合型组	47	2.31±0.30	16.51±1.62	16.95±2.09

组别	例数	CD4⁺CD25^highFoxP3⁺Treg(%)	IFN-γ(pg/mL)	IL-10(pg/mL)
干酪样型组	21	2.29±0.52	16.28±0.75	17.05±2.13
增殖型组	32	2.36±0.14	17.19±3.35	16.01±2.45
混合型组	47	2.31±0.30	16.51±1.62	16.95±2.09

淋巴结核患者外周血CD4⁺CD25^highFoxP3⁺调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义

Levels of CD4⁺CD25^highFoxP3⁺ regulatory T lymphocytes in peripheral blood and plasma IFN-γ, IL-10 of patients with lymph tuberculosis and their clinical significance

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淋巴结核患者外周血CD4+CD25highFoxP3+调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义

Levels of CD4+CD25highFoxP3+ regulatory T lymphocytes in peripheral blood and plasma IFN-γ, IL-10 of patients with lymph tuberculosis and their clinical significance

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淋巴结核患者外周血CD4⁺CD25^highFoxP3⁺调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义

Levels of CD4⁺CD25^highFoxP3⁺ regulatory T lymphocytes in peripheral blood and plasma IFN-γ, IL-10 of patients with lymph tuberculosis and their clinical significance