弥漫性大B细胞淋巴瘤免疫表型分类与c-MYC重排的相关性研究

doi:10.3969/j.issn.1673-8640.2013.04.014

检验医学 ›› 2013, Vol. 28 ›› Issue (4): 308-311.DOI: 10.3969/j.issn.1673-8640.2013.04.014

弥漫性大B细胞淋巴瘤免疫表型分类与c-MYC重排的相关性研究

王志林¹,姚玉华²,李桑¹,孙冠星¹

1. 常州市第一人民医院血液实验室，江苏常州 213003；2. 常州市第一人民医院检验科，江苏常州 213003

收稿日期:2012-03-13 修回日期:2012-04-19 出版日期:2013-04-30 发布日期:2013-04-05
作者简介:王志林，男，1972年生，硕士，副主任技师，主要从事血液病研究。

Research on correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma

1. Department of Hematology， the First People′s Hospital of Changzhou，Jiangsu Changzhou 213003,China; 2. Department of Clinical Laboratory，the First People′s Hospital of Changzhou，Jiangsu Changzhou 213003，China

Received:2012-03-13 Revised:2012-04-19 Online:2013-04-30 Published:2013-04-05

摘要/Abstract

摘要： 目的　探讨弥漫性大B细胞淋巴瘤(DLBCL)免疫表型分类与c-MYC重排的相关性。方法　根据淋巴瘤细胞免疫标志物CD10、B细胞淋巴瘤原癌基因-6（BCL-6）及多发性骨髓瘤原癌基因-1（MUM-1）的表达情况，将46例DLBCL分为生发中心B细胞样（GCB）与非生发中心B细胞样（non-GCB）2类，用荧光原位杂交（FISH）技术检测GCB类与non-GCB类中c-MYC重排情况。结果　46例标本中共有4例（8.7%）发生c-MYC重排，其中20例GCB类中发现4例（20.0%）c-MYC重排，而26例non-GCB类中未发现c-MYC重排。GCB类和non-GCB类中c-MYC重排差异有统计学意义（P＜0.05）。结论　GCB类DLBCL 患者c-MYC重排发生率较高，c-MYC重排是一部分GCB类DLBCL预后差的原因。FISH技术能快速、准确、灵敏地检测出c-MYC重排。

关键词: c-MYC重排, 弥漫性大B细胞淋巴瘤, 荧光原位杂交

Abstract: Objective　To investigate the correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma (DLBCL). Methods　According to the lymphoma cell immune marker CD10，B cell lymphoma 6 (BCL-6) and multiple myeloma oncogene 1 (MUM-1) expression levels，46 cases of DLBCL were classified into germinal center B cell-like(GCB) group and non-germinal center B cell-like(non-GCB) group. c-MYC rearrangement status was analyzed by fluorescence in situ hybridization (FISH) in GCB and non-GCB groups. Results　Of the 46 DLBCL cases，4 cases (8.7%) had c-MYC rearrangement. The 4 cases of c-MYC rearrangement belonged to the GCB group. There was no c-MYC rearrangement in non-GCB group. The c-MYC rearrangement had statistical significance between GCB and non-GCB groups (P＜0.05). Conclusions　The GCB group in patients with DLBCL has a high c-MYC rearrangement，and c-MYC rearrangement is a poor predictor for a part of GCB group. FISH is a rapid，accurate and sensitive technique for the detection of c-MYC rearrangement.

Key words: c-MYC rearrangement, Diffuse large B-cell lymphoma, Fluorescence in situ hybridization

王志林1，姚玉华2，李桑1，孙冠星1. 弥漫性大B细胞淋巴瘤免疫表型分类与c-MYC重排的相关性研究[J]. 检验医学, 2013, 28(4): 308-311.

WANG Zhilin 1，YAO Yuhua 2，LI Sang 1，SUN Guanxing 1. Research on correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma[J]. , 2013, 28(4): 308-311.

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弥漫性大B细胞淋巴瘤免疫表型分类与c-MYC重排的相关性研究

Research on correlation between immunophenotype and c-MYC rearrangement in diffuse large B-cell lymphoma

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