Table of Content

30 August 2018, Volume 33 Issue 8

Previous Issue    Next Issue

Orginal Article

Roles of serum 25-hydroxyvitamin D and bone mineral density in children with obesity

LIN Xiangquan, CHEN Hong

2018, 33(8):  669-673.  DOI: 10.3969/j.issn.1673-8640.2018.08.001

Asbtract ( 241 )   HTML ( 4)   PDF (782KB) ( 318 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the relations of 25-hydroxyvitamin D [25（OH）D] and bone mineral density with blood lipid,blood glucose and insulin resistance in children with simple obesity.Methods The levels of 25（OH）D,fasting blood glucose（FBG）,fasting insulin（FINS）,total cholesterol（TC）,triglyceride（TG）,high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C） and uric acid（UA）were determined in 109 children with simple obesity（obesity group） and 104 healthy children（healthy control group）. The homeostasis model assessment for insulin resistance（HOMA-IR） was calculated. The speed of sound（SOS） of left tibial bone was measured by ultrasonography. The obesity group was classified into mild obesity,moderate obesity and severe obesity groups according to body mass index （BMI）. The levels of 25（OH）D and SOS among the 3 groups were compared. The correlation between 25（OH）D and other indicators was evaluated by Pearson linear correlation analysis.Results The UA,TG,TC,LDL-C and FBG in obesity group were higher than those in healthy control group（P<0.05）,while 25（OH）D and SOS were lower（P<0.05）. In obesity group,25（OH）D was negatively correlated to TG（r=-0.279,P<0.05）,while there was no correlation between 25（OH）D and FINS,FBG,HOMA-IR,UA,TC,HDL-C and LDL-C（r=-0.144,-0.027,-0.144,-0.049,-0.106,0.021 and -0.137,P>0.05）. The levels of 25（OH）D and SOS in mild obesity,moderate obesity and severe obesity groups were gradually decreased with the degrees of obesity with statistical significance（P<0.05）.Conclusions The levels of 25（OH）D and SOS are gradually decreased in children with simple obesity with the degrees of obesity. Vitamin D deficiency may be related to obesity.

Changes of clinical laboratory determination indicators in male androgenic alopecia patients

GONG Jian, LIU Fang, GAO Peng, XU Jianfeng

2018, 33(8):  674-678.  DOI: 10.3969/j.issn.1673-8640.2018.08.002

Asbtract ( 259 )   HTML ( 4)   PDF (785KB) ( 409 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the changes of clinical laboratory determination indicators in male androgenic alopecia（MAA） patients,and to provide new clues for the diagnosis and treatment of MAA.Methods A total of 52 clinical laboratory determination indicators of thyroid-related markers,tumor markers,biochemical indicators,immune indicators and blood viscosity were determined in 430 MAA patients and 617 healthy subjects（healthy control group）. The results of the 2 groups were analyzed comparatively.Results The level of insulin（INS）was（10.49±0.86）μU/ mL,the level of free prostate-specific antigen（f-PSA） was（0.45±0.03）μg/L,the ratio of f-PSA/total prostate-specific antigen（t-PSA） was 0.31±0.02,the level of apolipoprotein E（apo E） was（1.12±0.01）g/L,and the whole blood viscosity：middle cut（50/s） was 7.40±0.04,which were higher than those in healthy control group（P<0.05,P<0.01）. There was no statistical significance for the other indicators between the 2 groups（P>0.05）.Conclusions Some clinical laboratory determination indicators have changes in MAA patients,which may have correlations with metabolic syndrome（MS）.

Accuracy evaluation of routine measurement procedure for serum homocysteine based on liquid chromatography-tandem mass spectrometry

ZHONG Qiaoling, SUN Ji, WENG Xuqi, FENG Ping, XIE Danyun

2018, 33(8):  679-682.  DOI: 10.3969/j.issn.1673-8640.2018.08.003

Asbtract ( 245 )   HTML ( 1)   PDF (835KB) ( 370 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the accuracy of enzymatic cycling method for serum homocysteine（Hcy） based on isotope-dilution liquid chromatography-tandem mass spectrometry（ID-LC/MS/MS） as a contrast method.Methods A total of 20 fresh serum samples were determined by enzymatic cycling method and ID-LC/MS/MS,and the results were compared. The levels of serum Hcy were adjusted until the results were consistent,according to the results of 5 working calibrations,which were determined by enzymatic cycling method and ID-LC/MS/MS.Results The results of standard reference material（SRM） 1950 determined by ID-LC/MS/MS were consistent with certificate value,and the relative bias was <1.0%. The linear equation of ID-LC/MS/MS and enzymatic cycling method was Y=1.204 1X+0.364 6（r²=0.987 9）,and the average bias was 24.0%. After adjusting,the linear equation was Y=0.988 6X+0.260 3（r²=0.999 7）,and the average bias was -0.09%.Conclusions The levels of serum Hcy determined by ID-LC/MS/MS are accurate,which can be used for the accuracy evaluation of routine measurement procedure for serum Hcy.

Role of Zn level in prostatic fluid for the diagnosis of different types of chronic prostatitis

ZHANG Bin, ZHOU Xiaolei

2018, 33(8):  683-685.  DOI: 10.3969/j.issn.1673-8640.2018.08.004

Asbtract ( 169 )   HTML ( 5)   PDF (794KB) ( 292 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the role of zinc（Zn） level in prostatic fluid for the diagnosis of different types of chronic prostatitis.Methods A total of 36 patients with chronic bacterial prostatitis（CBP） and 36 patients with non-chronic bacterial prostatitis（NCBP） were enrolled,and 40 healthy males were enrolled as healthy control group. Zn and C-reactive protein（CRP） levels were determined. Peripheral blood white blood cell（WBC） count was determined as well. The role of Zn level in prostatic fluid for the diagnosis of CBP was evaluated by receiver operating characteristic（ROC） curve. The correlations of Zn level in prostatic fluid with CRP level and WBC count were evaluated by Pearson correlation analysis.Results The levels of Zn in prostatic fluid in CBP,NCBP and healthy control groups were increased in turn（P<0.05）. The CRP level,WBC count and neutrophil absolute value（NEUT#） in CBP group were higher than those in NCBP and healthy control groups（P<0.05）. There was no statistical significance between NCBP group and healthy control group（P>0.05）. ROC curve analysis showed that the area under the curve of Zn level in prostatic fluid for the diagnosis of CBP was 0.845,the optimal cut-off value was 502.6 mg/L,the sensitivity was 83.5%,and the specificity was 79.3%. The Zn level in prostatic fluid was negatively correlated with CRP level and WBC count（r=-0.521 and -0.125,P<0.05）.Conclusions Zn level in prostatic fluid can help with diagnosing chronic prostatitis. Combined with CRP level and WBC count,it can assist to distinguish different types of chronic prostatitis.

Correlation of angiogenesis regulation factors and oxidative stress markers with adverse pregnancy in preeclampsia patients

LIU Sichao, YAN Hongli

2018, 33(8):  686-691.  DOI: 10.3969/j.issn.1673-8640.2018.08.005

Asbtract ( 186 )   HTML ( 11)   PDF (793KB) ( 299 )  

Figures and Tables | References | Related Articles | Metrics

Objective To assess the correlation of angiogenesis regulation factors [placental growth factor（PLGF） and soluble fms-like tyrosine kinase-1（sFlt-1）] and oxidative stress markers [8-epi-prostaglandin F2alpha（8-epi-PGF2α） and total antioxidant capacity（TAC）] with adverse pregnancy in gestational hypertension（GH） and preeclampsia（PE） patients.Methods Pregnant women who received antenatal care,including 65 GH patients and 84 PE patients,and 50 healthy women were enrolled. PLGF,sFlt-1 and 8-epi-PGF2α were determined by enzyme-linked immunosorbent assay（ELISA）. TAC was measured by ferric reducing antioxidant power（FRAP）. The characteristics and pregnancy outcomes before and after delivery were collected.Results At baseline（within 24 h）,the levels of sFlt-1 and 8-epi-PGF2α and sFlt-1/PLGF ratio in control,GH and PE groups increased in turn（P<0.001）,while the levels of PLGF and TAC decreased in turn（P<0.001）. Compared with those at baseline,the levels of TAC and PLGF in control,GH and PE groups were increased in 48 h after delivery（P<0.05,P<0.001）,while sFlt-1 and 8-epi-PGF2α levels and sFlt-1/PLGF ratio were decreased（P<0.001）. The proportion of fetal hip exposure during delivery in PE group was higher than those in control and GH groups（P<0.05）. The proportions of placental previa,placental exfoliation,intrauterine fetal death（IUFD）,intrauterine growth retardation（IUGR）,prenatal hemorrhage（APH） and preterm premature rupture of membranes（PPROM） were higher in PE group than those in control group（P<0.05）. The cesarean section rates in PE and GH groups were higher than that in control group（P<0.05）. The stillbirth,premature delivery,postpartum hemorrhage（PPH）and maternal mortality in PE group were higher than those in control group（P<0.05）. The neonatal birth weight for patients with PE and IUGR was lower than those for patients with severe PE,PE and GH and women with normal pregnancy（P<0.001）. The sFlt-1 and 8-epi-PGF2α levels and sFlt-1/PLGF ratios in PE combined with IUFD,PE combined with placental exfoliation,PE combined with IUGR,PE combined with placental previa and hemolysis,elevated liver enzymes and low platelet（HELLP） syndrome were higher than those in PE patients（P<0.05）. There was a negative correlation of IUGR,placental exfoliation/previa,IUFD,stillbirth and PPH with PLGF and TAC（P<0.05）,but there were positive correlations of IUGR,placental exfoliation/previa,IUFD,stillbirth and PPH with sFlt-1 and 8-epi-PGF2α in PE group（P<0.05）.Conclusions The imbalance between the levels of angiogenesis regulation factors and oxidative stress markers correlates with adverse pregnancy in PE patients,which can be used as an early warning of adverse pregnancy.

Clinical characteristic of cerebrospinal fluid for non-small cell lung cancer patients with leptomeningeal metastasis

FU Xiaoling, CHEN Kun, XU Jiaxue, GUAN Ming

2018, 33(8):  692-696.  DOI: 10.3969/j.issn.1673-8640.2018.08.006

Asbtract ( 186 )   HTML ( 7)   PDF (804KB) ( 440 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the role of cerebrospinal fluid clinical characteristic for non-small cell lung cancer（NSCLC） patients with leptomeningeal metastasis（LM）.Methods A total of 31 patients with NSCLC LM and 30 patients with primary central nervous system lymphoma（PCNSL） were enrolled. Their imaging characteristics were compared,and serum levels of alpha-fetoprotein（AFP）,carcinoembryonic antigen（CEA）,carbohydrate antigen（CA） 15-3 and CA19-9 were determined by chemiluminescence. The cerebrospinal fluid glucose,total protein and chloride level were determined by hexokinase kinase,bromcresol green/biuret and ion selective electrode methods,respectively. The levels of interleukin （IL）-6 and IL-10 in cerebrospinal fluid were determined by enzyme-linked immunosorbent assay. The determination rates of cerebrospinal fluid tumor cells according to cytology between the 2 groups were compared.Results There was no statistical significance in sex,age and imaging between NSCLC LM and PCNSL groups（P>0.05）. Serum levels of AFP,CEA and CA15-3 in NSCLC LM group were higher than those in PCNSL group（P<0.05）,and CA19-9 had no statistical significance between the 2 groups（P>0.05）. There was no statistical significance in cerebrospinal fluid pressure,nucleated cell count,chloride level and IL-6 between the 2 groups（P>0.05）,while cerebrospinal fluid glucose and IL-10 levels and IL-6/IL-10 ratio in NSCLC LM group had statistical significance compared with PCNSL group（P<0.05）.Conclusions The diagnosis of NSCLC LM should be integrated with clinical characteristics,imaging findings,serum tumor markers' levels and cerebrospinal fluid IL-6 level and IL-6/IL-10 ratio,which could improve the diagnosis of NSCLC LM.

Expression levels and their roles of serum IL-1β,IL-6,TNF-α,IL-17 and IL-23 of schizophrenia patients

LI Zebing, LI Dong, YANG Xuesong, SUN Zujun, LIN Ping, WANG Feng, LIN Feiran, XU Xiao, XIE Hongtao

2018, 33(8):  697-701.  DOI: 10.3969/j.issn.1673-8640.2018.08.007

Asbtract ( 187 )   HTML ( 11)   PDF (915KB) ( 379 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the changes of serum interleukin（IL）-1β,IL-6,tumor necrosis factor-alpha（TNF-α）,IL-17 and IL-23 levels in schizophrenia patients,and to evaluate the roles in the auxiliary diagnosis of schizophrenia.Methods A total of 85 patients with schizophrenia were enrolled as case group,and 85 healthy subjects were enrolled as control group. IL-1β,IL-6,TNF-α,IL-17 and IL-23 were determined by enzyme-linked immunosorbent assay（ELISA）. The changes of case group before and after treatment for 3 months were evaluated. The auxiliary diagnosis role of serum cytokines was evaluated by receiver operating characteristic（ROC） curve. Pearson correlation analysis was used to analyze the correlation between the levels of serum cytokines and the positive and negative syndrome scale（PANSS） score.Results Before treatment,serum levels of IL-1β,IL-6,TNF-α,IL-17 and IL-23 in case group were higher than those in control group（P<0.01）. After treatment for 3 months,serum levels of IL-1β,IL-6,TNF-α,IL-17 and IL-23 in case group were lower than those before treatment（P<0.01）,and there was no statistical significance compared with control group（P>0.05）. The areas under ROC curves（AUC） of serum IL-1β,IL-6,TNF-α,IL-17 and IL-23 were 0.723,0.772,0.686,0.685 and 0.679. Serum IL-1β,IL-17 and IL-23 were positively correlated with PANSS total score,positive symptom score,general psychopathology score and supplemental aggression risk subscale score TNF-α was positively correlated with positive symptom score（P<0.05）,and IL-6 was positively correlated with PANSS total score and negative symptom score（P<0.05）.Conclusions The activation of serum cytokines（IL-1β,IL-6,TNF-α,IL-17 and IL-23） exists in schizophrenia patients,serum cytokines play roles in the auxiliary diagnosis of schizophrenia,and these levels are correlated with clinical symptoms.

Tumor diagnostic rate and tumor marker determination for 84 patients with typical paraneoplastic antibody positive

LIU Xia, WU Yanyan, WANG Jinling, LAN He, LIU Li, WANG Peichang

2018, 33(8):  702-706.  DOI: 10.3969/j.issn.1673-8640.2018.08.008

Asbtract ( 282 )   HTML ( 9)   PDF (787KB) ( 431 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the cause for low tumor diagnostic rate for patients with typical paraneoplastic antibody positive.Methods Neuronal antigen IgG antibody was determined by immunoblotting assay. Tumor markers were determined by chemiluminescence. The clinical data,including the results of tumor biomarkers and diagnosis for patients with typical paraneoplastic antibody positive,were collected through terminal electronic medical record.Results There were 15（17.9%）,0（0.0%）,18（21.4%）,26（31.0%）,9（10.7%） and 12（14.3%） positive cases of anti-Hu,anti-Ri,anti-Yo,anti-PNMA2（Ma2/Ta）,anti-CV2 and anti-Amphiphysin antibodies,respectively. There were 3 positive cases of both anti-CV2 and anti-PNMA2（Ma2/Ta） antibodies and 1 positive case of both anti-CV2 and anti-Hu antibodies. Tumors were found in 14.3% patients of 84 cases of typical paraneoplastic antibody positive. Moreover,7.4% patients with positive anti-Hu or anti-Amphiphysin antibodies were diagnosed with tumors. Tumor markers were not determined in 14 of 72 patients（19.4%） who were not diagnosed with tumors. The negative rate and the positive rate of tumor markers were 46.6%（27 cases） and 53.4%（31 cases） in 58 patients who were not diagnosed with tumors,respectively.Conclusions The tumor missed diagnosis rate is high in patients with typical paraneoplastic antibody positive,which could be due to the missed determination of tumor markers,no further determination of tumors in patients with normal tumor marker determination results and inaccurate site of tumor examination.

Epidemiological distribution and drug resistance of Aeromonas and Plesiomonas in the intestine infection

LI Shirong, JIANG Xiaofei

2018, 33(8):  707-710.  DOI: 10.3969/j.issn.1673-8640.2018.08.009

Asbtract ( 281 )   HTML ( 8)   PDF (802KB) ( 268 )  

Figures and Tables | References | Related Articles | Metrics

Objective To study the epidemiological distribution and drug resistance of Aeromonas and Plesiomonas in the intestine infection,and to provide a reference for clinical treatment.Methods The epidemiological distribution and drug resistance of Aeromonas（46 isolates） and Plesiomonas（10 isolates） isolated from feces specimens from 2007 to 2016 were analyzed retrospectively.Results A total of 46 isolates of Aeromonas were isolated from feces specimens,accounting for 22.0% and ranking No.3 in all intestinal isolates. There were 27 isolates of Aeromonas hydrophila（58.7%）,followed by Aeromonas sobria（10 isolates,21.7%） and Aeromonas vienna（9 isolates,19.6%）. There were 10 isolates of Plesiomonas,accounting for 4.8% of all intestinal isolates. There were 27 of 56 isolates isolated from Department of Intestine,10 isolates from Department of Gastroenterology and 19 isolates from other departments. The determination rates for males and females were 42.9% and 57.1%,respectively. It was able to isolate Aeromonas and Plesiomonas all the year round,and the highest isolation rate was 71.4% from July to October. Aeromonas had various degrees of drug resistance to most antibiotics. The drug resistance rates to amikacin,gentamycin,ceftazidime,aztreonam,ciprofloxacin,sulfamethoxazole-trimethoprim,piperacillin-tazobactam and cefepime were 2.2%,6.5%,6.5%,4.3%,6.5%,10.7%,19.6% and 6.5%,respectively. The isolates of Aeromonas were sensitive to meropenem,and 2 isolates were intermediate drug resistant to imipenem. Plesiomonas had various degrees of drug resistance to amikacin,gentamycin,ciprofloxacin and sulfamethoxazole-trimethoprim. The drug resistance rates were 10.0%,20.0%,30.0% and 40.0%,respectively,while Plesiomonas were sensitive to ceftazidime,aztreonam,imipenem,meropenem,piperacillin-tazobactam and cefepime.Conclusions The isolation rates of Aeromonas and Plesiomonas are high,and there were different degrees of drug resistance to various antibiotics,such as quinolones,the 3rd generation and the 4th generation cephalosporins,sulfonamides,aminoglycosides and monocyclic beta-lactams. There exists intermediate drug resistance to imipenem.

Bloodstream infection and pathogen drug resistance in hemodialysis patients

WANG Xiaolong, HUANG Zuofu, ZHANG Shuxia, YANG Yulin

2018, 33(8):  711-715.  DOI: 10.3969/j.issn.1673-8640.2018.08.010

Asbtract ( 242 )   HTML ( 8)   PDF (784KB) ( 227 )  

Figures and Tables | References | Related Articles | Metrics

Objective To analyze the bloodstream infection and pathogen drug resistance in hemodialysis patients in hemodialysis center retrospectively,and to provide a reference for infection control.Methods Fever patients in the hemodialysis center from January 2013 to December 2016 were enrolled. Antibiotic susceptibility test and pathogen identification were performed by Vitek 2 compact system（BioMerieux）. The results were analyzed according to the Clinical and Laboratory Standards Institute（CLSI）M100-S26. SPSS 17.0 software was used for statistical analysis.Results A total of 86 isolates of pathogens were isolated from 816 specimens（10.5%）,but there was no significant increasing or decreasing trend from 2013 to 2016（P>0.25）. Gram-positive cocci accounted for 54.7%（47/86）,and the main pathogen was Staphylococcus aureus. Gram-negative bacilli accounted for 37.2%（32/86）,and the main pathogen was Escherichia coli. Only 8.1%（7/86） isolates were fungi. All of the Gram-positive cocci were sensitive to vancomycin and linezolid,but the susceptibility to penicillin was low. All of the Gram-negative bacilli were sensitive to amikacin and meropenem,but the susceptibility to ampicillin was low. The fungi were sensitive to all of the antibiotics determined. Only 19.8%（17/86） isolates were multi-drug resistant organisms.Conclusions Dialysis scheme should base on individual status to avoid blood infection. Most of the pathogens isolated from patients in the hemodialysis center are Gram-positive cocci,and the determination rate of multi-drug resistant organisms is not high. Empirical treatment will be effective,but medication should be adjusted according to drug susceptibility results.

Infection status analysis of multi-drug resistant bacteria in Renmin Hospital of Wuhan University

GUO Jing, LI Congrong

2018, 33(8):  716-721.  DOI: 10.3969/j.issn.1673-8640.2018.08.011

Asbtract ( 337 )   HTML ( 8)   PDF (774KB) ( 419 )  

Figures and Tables | References | Related Articles | Metrics

Objective To study the distribution and drug resistance of multi-drug resistant（MDR） bacteria in Renmin Hospital of Wuhan University,and to provide a reference for using antibiotics rationally and controlling nosocomial infection.Methods The MDR bacteria isolated from Renmin Hospital of Wuhan University were analyzed retrospectively from March 2016 to March 2017.Results A total of 7 402 clinical isolates were collected. There were 1 701 isolates of MDR bacteria,and the determination rate was 22.99%. The top 7 MDR bacteria were extended-spectrum beta-lactamase-Escherichia coli（ESBL-Eco） accounting for 39.15%（666/1 701）,carbapenem-resistant Acinetobacter baumannii（CRAB） accounting for 22.52%（382/1 701）,methicillin-resistant Staphylococcus aureus（MRSA） accounting for 14.87%（253/1 701）,extended-Spectrum beta-lactamase-Klebsiella pneumoniae（ESBL-Kpn）accounting for 7.34%（125/1 701）,carbapenem-resistant Enterobacteriaceae（CRE） accounting for 7.35%（125/1 701）,carbapenem-resistant Pseudomonas aeruginosa（CRPA） accounting for 6.46%（110/1 701） and vancomycin-resistant Enterococcus（VRE） accounting for 0.76%（13/1 701）. The MDR bacteria were mainly distributed in general surgery,intensive medical department,neurology and respiratory medicine departments. Among 41.45% of sputum samples and 34.83% of midstream urine samples,MDR bacteria were found. The drug resistance rates of Escherichia coli to imipenem and meropenem were 1.43% and 1.31%,respectively. The drug resistance rates of Klebsiella pneumoniae to imipenem,meropenem and amikacin were 11.82%,11.82% and 10.52%,respectively. Except colistin,tigecycline and minocycline,the drug resistance rates of Acinetobacter baumannii to the other antibiotics were higher than 70%. The drug resistance rates of Pseudomonas aeruginosa to various antibiotics were lower than 23%. There were 8 isolates and 4 isolates of vancomycin- and teicoplanin-resistant Staphylococcus aureus and Enterococcus faecium,respectively. There was no vancomycin- and teicoplanin-resistant Enterococcus faecalis.Conclusions The status of MDR bacterium infection is grim,and it has become the main pathogen of nosocomial infection,so the drug resistance monitoring of MDR bacteria for the rational use of antibiotics and nosocomial infection control is of great significance.

Determination of coagulation-fibrinolytic system markers in patients with malignant tumor and its role

CHEN Wenxin, LI Qiang, XUE Yingjun, SHAO Huaqing, LI Zhen, LU Wenqian, HU Xiaobo

2018, 33(8):  722-726.  DOI: 10.3969/j.issn.1673-8640.2018.08.012

Asbtract ( 173 )   HTML ( 6)   PDF (777KB) ( 594 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the relationships of plasma thrombomodulin（TM）,thrombin-antithrombin complex（TAT）,plasmin-alpha 2 plasmin inhibitor complex（PIC） and tissue plasminogen activator-plasminogen activator inhibitor-1 complex（t-PAI-C） with tumors.Methods The levels of plasma TM,TAT,PIC and t-PAI-C were determined by chemiluminescence enzyme immunoassay in 164 malignant tumor patients（81 cases of respiratory tract cancer and 83 cases of gastrointestinal cancer） and 52 healthy subjects（healthy control group）. The difference of plasma TM,TAT,PIC and t-PAI-C between malignant tumor and healthy control groups was evaluated,and the difference between tumor metastasis and non-tumor metastasis groups was analyzed as well.Results The levels of TM,TAT,PIC and t-PAI-C in malignant tumor group were higher than those in healthy control group（P<0.01）. The levels of TM,TAT and PIC in respiratory tract cancer group were higher than those in healthy control group（P<0.01）,and there was no statistical significance for t-PAI-C between the 2 groups（P>0.05）. There was statistical significance for TM,TAT,PIC and t-PAI-C between gastrointestinal cancer group and healthy control group（P<0.01）. In malignant tumor group,TAT,PIC and t-PAI-C were higher in tumor metastasis group than those in non-tumor metastasis group（P<0.01）,while TM had no statistical significance between the 2 groups（P>0.05）. In respiratory tract cancer and gastrointestinal cancer groups,the levels of TAT,PIC and t-PAI-C in tumor metastasis group were higher than those in non-tumor metastasis group（P<0.01）,while there was no statistical significance for TM（P>0.05）.Conclusions Malignant tumor patients have different degrees of vascular endothelial injury and coagulation-fibrinolytic system disorder. Plasma TM,TAT,PIC and t-PAI-C can be used as indicators for malignant tumor progression,curative effect and prognosis.

Thalassemia screening strategies

TAN Shi, LI Qiuhong, CHEN Xia, LI Chunli

2018, 33(8):  730-733.  DOI: 10.3969/j.issn.1673-8640.2018.08.013

Asbtract ( 422 )   HTML ( 2)   PDF (816KB) ( 393 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the roles of mean corpuscular volume（MCV） determination,mean corpuscular hemoglobin（MCH） determination and hemoglobin（Hb）electrophoresis in thalassemia screening.Methods A total of 1 017 patients with thalassemia diagnosed by molecular biologic technique（thalassemia group） and 842 patients without thalassemia（control group） were enrolled for MCV and MCH determinations and Hb electrophoresis. The roles of MCV determination,MCH determination and Hb electrophoresis in thalassemia screening were evaluated.Results The levels of MCV and MCH in thalassemia group were lower than those in control group（P<0.05）. Compared with control group,the levels of HbA₂ were higher in β- and αβ-thalassemia groups and lower in α-thalassemia group（P<0.05）. The level of HbF in β-thalassemia group was higher than that in control group（P<0.05）. There was no statistical significance between static-type α-thalassemia and control groups（P>0.05）. The levels of Hb,MCV,MCH and HbA₂ were lower in light-type and intermediate-type α-thalassemia groups compared with control group（P<0.01）. In α-thalassemia group,MCV determination and Hb electrophoresis had high rates of missed diagnosis. The rates of missed diagnosis were 42.38% for MCV determination,41.56% for Hb electrophoresis and 18.38% for MCH determination. The rates of missed diagnosis of MCV determination,MCH determination and Hb electrophoresis were 64.20%,22.19% and 59.47% in static-type α-thalassemia group and 14.12%,12.98% and 19.47% in light-type α-thalassemia group,respectively. The sensitivities of MCV determination,MCH determination and Hb electrophoresis were 68.34%,83.09% and 74.72%,and the specificities were 82.30%,68.17% and 74.11%,respectively. The sensitivity of MCV and MCH parallel determinations was 83.28%,and the specificity of the combined determination of MCV and MCH parallel determinations with Hb electrophoresis was 85.04%.Conclusions MCV and MCH parallel determinations can improve the sensitivity,and the combined determination of MCV and MCH parallel determinations with Hb electrophoresis can improve the specificity in thalassemia screening.

Thalassemia screening strategies

TAN Shi, LI Qiuhong, CHEN Xia, LI Chunli

2018, 33(8):  730-733.  DOI: 10.3969/j.issn.1673-8640.2018.08.013

Asbtract ( 115 )   HTML ( 0)   PDF (794KB) ( 243 )  

References | Related Articles | Metrics

Objective To investigate the roles of mean corpuscular volume（MCV） determination,mean corpuscular hemoglobin（MCH） determination and hemoglobin（Hb）electrophoresis in thalassemia screening.Methods A total of 1 017 patients with thalassemia diagnosed by molecular biologic technique（thalassemia group） and 842 patients without thalassemia（control group） were enrolled for MCV and MCH determinations and Hb electrophoresis. The roles of MCV determination,MCH determination and Hb electrophoresis in thalassemia screening were evaluated.Results The levels of MCV and MCH in thalassemia group were lower than those in control group（P<0.05）. Compared with control group,the levels of HbA₂ were higher in β- and αβ-thalassemia groups and lower in α-thalassemia group（P<0.05）. The level of HbF in β-thalassemia group was higher than that in control group（P<0.05）. There was no statistical significance between static-type α-thalassemia and control groups（P>0.05）. The levels of Hb,MCV,MCH and HbA₂ were lower in light-type and intermediate-type α-thalassemia groups compared with control group（P<0.01）. In α-thalassemia group,MCV determination and Hb electrophoresis had high rates of missed diagnosis. The rates of missed diagnosis were 42.38% for MCV determination,41.56% for Hb electrophoresis and 18.38% for MCH determination. The rates of missed diagnosis of MCV determination,MCH determination and Hb electrophoresis were 64.20%,22.19% and 59.47% in static-type α-thalassemia group and 14.12%,12.98% and 19.47% in light-type α-thalassemia group,respectively. The sensitivities of MCV determination,MCH determination and Hb electrophoresis were 68.34%,83.09% and 74.72%,and the specificities were 82.30%,68.17% and 74.11%,respectively. The sensitivity of MCV and MCH parallel determinations was 83.28%,and the specificity of the combined determination of MCV and MCH parallel determinations with Hb electrophoresis was 85.04%.Conclusions MCV and MCH parallel determinations can improve the sensitivity,and the combined determination of MCV and MCH parallel determinations with Hb electrophoresis can improve the specificity in thalassemia screening.

Determinations of Candida,Cryptococcus neoformans and ERG11 gene point mutations by gene chip

WANG Jinghua, YU Peijuan, GE Ping, ZHANG Qiangqiang, XIAO Yanqun, WANG Qingzhong, XU Rong, CHEN Rong, LIU Xuejie, JIANG Lingli, WANG Xueliang, WANG Hualiang

2018, 33(8):  742-748.  DOI: 10.3969/j.issn.1673-8640.2018.08.016

Asbtract ( 276 )   HTML ( 5)   PDF (1348KB) ( 238 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the determinations of Candida,Cryptococcus neoformans and ERG11 gene point mutations by gene chip.Methods The primers and oligonucleotide probes of 5.8 S rDNA and 28S rDNA of internal transcribed spacer-2（ITS-2） gene of 5 species of pathogenic Candida and Cryptococcus neoformans were synthesized,and the oligonucleotide microarray was prepared for identification. The specific primers and oligonucleotide probes of point mutations in ERG11 gene were synthesized,and ERG11 gene was amplified by asymmetric fluorescence polymerase chain reaction（PCR）. The products were analyzed by oligonucleotide microarray.Results A total of 4 point mutations of ERG11 gene were amplified from 12 isolates of drug resistant Candida by asymmetric fluorescence PCR with specific primers,and 5 species of pathogenic Candida and Cryptococcus neoformans were identified by gene chip successfully.Conclusions The oligonucleotide microarray established in this study can be used to identify the clinical isolated pathogenic Candida and Cryptococcus neoformans.

Strategies for syphilis serological determination

WANG Qin

2018, 33(8):  749-751.  DOI: 10.3969/j.issn.1673-8640.2018.08.017

Asbtract ( 232 )   HTML ( 6)   PDF (774KB) ( 268 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the roles of tolulized red unheated serum test（TRUST）,Treponema pallidum particle agglutination assay（TPPA）and chemiluminescence immunoassay（CLIA）for syphilis serological determination,and to formulate reasonable determination strategies.Methods The results of syphilis determination in 11 034 patients（excluding revisits） were analyzed retrospectively.Results The results of TPPA and CLIA had statistical significance for Treponema pallidumdetermination（P<0.01）. The specificities of the 3 methods were high,TPPA and CLIA had high sensitivities,and the sensitivity of TRUST was low.Conclusions The 3 methods have their advantages and disadvantages. Different determination strategies should be chosen according to different purposes.

Internal imprecision status of routine chemical determination items in Anhui laboratories

DONG Huijun, FAN Renzhi, ZHOU Hongyan, CHEN Xuemin, WU Yun, SHEN Zuojun

2018, 33(8):  752-754.  DOI: 10.3969/j.issn.1673-8640.2018.08.018

Asbtract ( 221 )   HTML ( 3)   PDF (864KB) ( 269 )  

Figures and Tables | References | Related Articles | Metrics

Objective To provide scientific data for supporting clinical practice by analyzing the internal imprecision status of clinical routine chemical determination items in laboratories.Methods The data of median level from the Internal Quality Control Program from 159 hospitals were collected and evaluated,and the total coefficients of variation for 21 clinical routine chemical determination items [albumin（Alb）,alkaline phosphatase（ALP）,alanine aminotransferase（ALT）,amylase（Amy）,aspartate aminotransferase（AST）,total calcium（Ca）,creatine kinase（CK）,chlorine（Cl）,creatinine（Cr）,gamma glutamyltransferase（GGT）,glucose（Glu）,potassium（K）,lactate dehydrogenase（LDH）,sodium（Na）,inorganic phosphate（P）,total bilirubin（TB）,total cholesterol（TC）,triglyceride（TG）,total protein（TP）,uric acid（UA） and urea] were analyzed. The consistency of 21 clinical routine chemical determination items was analyzed according to WS/T 403—2012 as standard.Results Except Cl and Ca,the imprecisions（medians） of other determination items were lower than the WS/T 403—2012 standard. The overall consistency rates of Cl,Ca,TP and urea were <60%. Grouped statistics for medical institutions showed more extreme data,among which the consistency rate of Ca had statistical significance between the tertiary and the secondary medical institutions（χ²=5.12,P<0.05）. There was no statistical significance for the consistency rates of the other determination items（P>0.05）.Conclusions The imprecision status of routine chemical determination items in Anhui meets clinical basic demand,and some determination items need to be improved.

Development of national standard materials of urea,uric acid and total protein in compound frozen human serum

YU Ting, SHEN Min, SUN Nan, QU Shoufang, HUANG Jie

2018, 33(8):  755-759.  DOI: 10.3969/j.issn.1673-8640.2018.08.019

Asbtract ( 175 )   HTML ( 2)   PDF (890KB) ( 402 )  

Figures and Tables | References | Related Articles | Metrics

Objective To develop the national standard materials of urea,uric acid and total protein in compound frozen human serum,to calibrate and evaluate conventional methods,and to improve the standardization of urea,uric acid and total protein determinations.Methods Serum samples with different levels and without hemolysis,lipemia and choloplania were collected. After filtration and sterilization,serum pools were packed in freezing tubes and stored in -70 ℃. The homogeneity was evaluated by single factor analysis of variance. The stability was evaluated by linear regression analysis. The value was assigned by reference methods or validated method,and the uncertainty was calculated. The commutability of 25 serum samples between reference method and 3 conventional analysis systems was evaluated.Results The F values of homogeneity for urea,uric acid and total protein were 1.071 4,1.339 9 and 1.275 0,respectively. All of them were <F_0.05. The stabilities of urea,uric acid and total protein in candidates can be last at least 37 d at -20 ℃,at least 27,37 and 37 d at 2-8 ℃ and at least 3,8 and 27 d at 20-25 ℃,respectively. The assigned values of candidates for urea,uric acid and total protein were（5.68±0.17） mmol/L（k=2）,（288.68±3.96）μmol/L（k=2）,（67.03±3.46）g/L（k=2）. The linearities for urea,uric acid and total protein for25 samples were all within 95% confidence interval range.Conclusions The homogeneity,stability and commutability of candidates all meet the requirements,and the assigned value is accurate and reliable.

Analytical quality requirement of range comparison for multiple biochemical analyzers in clinical laboratories

XIE Wenge, LI Wen, HUANG Ning, LI Jinxing

2018, 33(8):  760-763.  DOI: 10.3969/j.issn.1673-8640.2018.08.020

Asbtract ( 295 )   HTML ( 3)   PDF (803KB) ( 304 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate biological variations,laboratory internal long-term imprecision and allowable coefficient of variation（CV） in range comparison,and to determine appropriate criteria for judgement.Methods According to WS/T 407—2012,the scheme of range comparison was established. With high-level and low-level of patients' fresh sera,19 biochemical items were determined by 3 biochemical instruments.Results For the 19 biochemical items,laboratory internal long-term imprecision and combined CV at the same level were < allowable CV,and 50% combined CV was >1/3 biological variation. Each comparison deviation was < allowable CV,and 50% comparison deviation was >1/3 biological variation. The 20% comparison deviation was > critical range.Conclusions Allowable CV can be used as a criterion for judgement,which is non-congener comparison（range comparison and standard deviation）. The critical range belongs to congener comparison,which is strict slightly and can be used as a criterion for the combined usage with allowable CV. 1/3 Biological variation belongs to congener comparison and is too strict to be used as a criterion for judgement.

Influence of antibiotic use on intestinal microecology of infants

SU Siting, WEN Hongyu, YUAN Zhenya, WANG Xiuying, WANG Guozhen, YU Chenjie

2018, 33(8):  764-767.  DOI: 10.3969/j.issn.1673-8640.2018.08.021

Asbtract ( 175 )   HTML ( 1)   PDF (792KB) ( 288 )  

References | Related Articles | Metrics

Human intestinal microecology has a vast number of bacteria,which plays a critical role in human health. At present,it is generally accepted that antibiotics have been one of the most common drugs in the world,and antibiotic use is associated with increasing risk for several diseases. Antibiotic use during infancy will induce imbalance in intestinal microecology,which is called dysbiosis. In the recent years,more and more researches have been known about the influence of antibiotics on intestinal microecology of infants. This review summarizes the effects of antibiotics on intestinal microecology of infants.

123456

2018, 33(8):  770-771.  DOI: 10.3969/j.issn.1673-8640.2018.08.023

Asbtract ( 261 )   HTML ( 1)   PDF (773KB) ( 538 )  

Figures and Tables | References | Related Articles | Metrics