Abstract:

Objective To investigate the clinical value of methylenetetrahydrofolate reductase（MTHFR） gene C677T polymorphism determination in H-type hypertension. Methods The MTHFR gene C677T polymorphism was determined by digital fluorescence molecular hybridization in 215 patients with hypertension and 113 healthy subjects，and the levels of serum homocysteine（Hcy） were determined. The patients with hypertension were classified into H-type group（143 cases） and non-H-type hypertension group（72 cases） according to Hcy levels. According to the severity of hypertension，they were classified into Grade Ⅰ，Ⅱ and Ⅲ groups（48，77 and 90 cases，respectively）. Serum Hcy levels were determined in patients with H-type hypertension before and after folic acid treatment for 6 months. Results Serum Hcy level in H-type hypertension group was higher than those in non-H-type hypertension group and healthy control group（P<0.01）. Serum Hcy level in Grade Ⅲ hypertension group was higher than those in Grade Ⅱ and Grade Ⅰ hypertension groups（P<0.05）. The frequencies of CC，TT genotype and C，T allele in H-type hypertension group were higher than those in non-H-type hypertension group and healthy control group（P<0.05）. The frequencies of TT genotype and T allele in Grade Ⅱ and Grade Ⅲ hypertension group were higher than those in Grade Ⅰ hypertension group（P<0.05）. In patients with H-type hypertension，serum Hcy level in TT genotype group was higher than those in CT genotype group and CC genotype group before and after folic acid treatment（P<0.01）. After folic acid treatment，serum Hcy levels of the 3 genotypes were lower than those before treatment（P<0.05）. Serum Hcy levels of TT genotype patients with H-type hypertension before and after folic acid treatment were higher than those of CT genotype patients and CC genotype patients（P<0.01）. Conclusions The MTHFR gene C677T polymorphism is related to the severity of hypertension，and its determination has clinical value for individualized treatment efficacy of H-type hypertension.

Key words: Methylenetetrahydrofolate reductase, Gene polymorphism, Homocysteine, H-type hypertension