Tracking detection analysis of 23 cases of SARS-CoV-2 nucleic acid single target positive samples in Zhengzhou

doi:10.3969/j.issn.1673-8640.2020.07.001

Abstract

Abstract:

Objective To perform tracking detection from the first detection of 23 cases of severe acute respiratory syndrome coronavirus 2（SARS-CoV-2） nucleic acid single target positive samples in Zhengzhou,and to provide a reference for clinical laboratories. Methods According to the Pneumonia Prevention and Control Plan for Novel Coronavirus Infection,nasal swab and oropharyngeal swab samples from suspected corona virus disease 2019 （COVID-19） patients were collected. The nucleic acid was extracted and detected by real-time fluorescence reverse transcription polymerase chain reaction（RT-PCR）. Totally,6 open reading frame lab（ORF1ab） gene and 17 nucleocapsid protein（N） gene single target positive samples were detected. Results Totally,8 samples were positive in the tracking detection,and the positive conversion rate was 34.78%. Conclusions The samples detected single target positive for 2 times should be treated seriously and detected for tracking detection.

Key words: Severe acute respiratory syndrome coronavirus 2, Corona virus disease 2019, Real-time fluorescence reverse transcription polymerase chain reaction, Single target positivity

CLC Number:

R446.1

NIU Weidong, XU Taibin, SUN Limei, AN Yi, SHI Jun, LI Yi, ZHAO Ruizhen. Tracking detection analysis of 23 cases of SARS-CoV-2 nucleic acid single target positive samples in Zhengzhou[J]. Laboratory Medicine, 2020, 35(7): 633-636.

Figures/Tables 3

References 13

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标本	第1次采样检测			第2次采样检测			第3次采样检测			第4次采样检测			第5次采样检测
标本	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果
1	N/A	35.56	重采	N/A	37.10	重采	31.06	32.13	阳性
2	N/A	40.68	重采	37.99	35.42	阳性
3	N/A	39.69	重采	34.17	33.68	阳性
4	N/A	39.65	重采	32.34	33.02	阳性
5	N/A	35.84	重采	35.59	34.74	阳性
6	N/A	34.99	重采	31.93	34.54	阳性
7	N/A	34.67	重采	34.21	33.07	阳性
8	N/A	35.85	重采	N/A	35.71	重采	N/A	30.60	重采	N/A	37.33	重采	N/A	N/A	阴性
9	N/A	38.80	重采	N/A	37.33	重采	N/A	N/A	阴性
10	N/A	38.05	重采	N/A	38.43	重采	N/A	N/A	阴性
11	N/A	37.44	重采	N/A	35.54	重采	N/A	N/A	阴性
12	N/A	33.11	重采	N/A	36.23	重采	N/A	N/A	阴性
13	N/A	30.26	重采	N/A	36.21	重采	N/A	N/A	阴性
14	N/A	38.11	重采	N/A	N/A	阴性
15	N/A	37.24	重采	N/A	N/A	阴性
16	N/A	35.77	重采	N/A	N/A	阴性
17	N/A	33.81	重采	N/A	N/A	阴性
18	37.60	N/A	重采	28.42	31.15	阳性
19	36.93	N/A	重采	N/A	N/A	阴性
20	36.69	N/A	重采	N/A	N/A	阴性
21	36.39	N/A	重采	N/A	N/A	阴性
22	36.13	N/A	重采	N/A	N/A	阴性
23	36.09	N/A	重采	N/A	N/A	阴性

标本	第1次采样检测			第2次采样检测			第3次采样检测			第4次采样检测			第5次采样检测
标本	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果	ORF1ab基因	N基因	结果
1	N/A	35.56	重采	N/A	37.10	重采	31.06	32.13	阳性
2	N/A	40.68	重采	37.99	35.42	阳性
3	N/A	39.69	重采	34.17	33.68	阳性
4	N/A	39.65	重采	32.34	33.02	阳性
5	N/A	35.84	重采	35.59	34.74	阳性
6	N/A	34.99	重采	31.93	34.54	阳性
7	N/A	34.67	重采	34.21	33.07	阳性
8	N/A	35.85	重采	N/A	35.71	重采	N/A	30.60	重采	N/A	37.33	重采	N/A	N/A	阴性
9	N/A	38.80	重采	N/A	37.33	重采	N/A	N/A	阴性
10	N/A	38.05	重采	N/A	38.43	重采	N/A	N/A	阴性
11	N/A	37.44	重采	N/A	35.54	重采	N/A	N/A	阴性
12	N/A	33.11	重采	N/A	36.23	重采	N/A	N/A	阴性
13	N/A	30.26	重采	N/A	36.21	重采	N/A	N/A	阴性
14	N/A	38.11	重采	N/A	N/A	阴性
15	N/A	37.24	重采	N/A	N/A	阴性
16	N/A	35.77	重采	N/A	N/A	阴性
17	N/A	33.81	重采	N/A	N/A	阴性
18	37.60	N/A	重采	28.42	31.15	阳性
19	36.93	N/A	重采	N/A	N/A	阴性
20	36.69	N/A	重采	N/A	N/A	阴性
21	36.39	N/A	重采	N/A	N/A	阴性
22	36.13	N/A	重采	N/A	N/A	阴性
23	36.09	N/A	重采	N/A	N/A	阴性

检测次数	基因类型		检测结果		总计
检测次数	ORF1ab	N	阳性	阴性	总计
2	10	6	7	9	16
3	6	0	1	5	6
≥4	1	0	0	1	1

检测次数	基因类型		检测结果		总计
检测次数	ORF1ab	N	阳性	阴性	总计
2	10	6	7	9	16
3	6	0	1	5	6
≥4	1	0	0	1	1

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