Abstract:

Objective To evaluate the role of fluorescent platelet（PLT-F） channel in the determination of abnormal platelet（PLT） count. Methods A total of 158 fasting peripheral blood specimens were collected,which were classified into low PLT group,small red blood cell interference group,large PLT group and PLT aggregation group. Using impedance（PLT-I）,fluorescence（PLT-F） and manual microscopy（PLT-M） methods,PLT counts were determined. The PLT-I and PLT-F results were compared with those of PLT-M. Results The within-run repeatabilities [coefficients of variation（CV）] of PLT count and immature platelet fraction（IPF） in low PLT,large PLT and small red blood cell interference groups were 4.94%,1.70%,0.80% and 4.81%,1.30%,2.74%,respectively. The determination coefficients（r²） of PLT-F with PLT-M in low PLT group,small red blood cell interference group and large PLT group were 0.856 8,0.822 3 and 0.771 0,respectively,and those of PLT-I with PLT-M were 0.679 1,0.775 4 and 0.592 6,respectively. Conclusions PLT-F channel has good within-run repeatability. PLT-F channel is a rapid and accurate retest way on determining abnormal PLT count.

Key words: Fluorescent platelet channel, Hematology analyzer, Abnormal platelet count, Manual microscopy