Objective To investigate the roles of serum biotin,vitamin B12（Vit B12） and folic acid（FA） in premature white hair disease. Methods A total of 80 patients with premature white hair disease and 80 healthy subjects（healthy control group） were enrolled. Their general data [sex,age,body mass index（BMI）,total protein（TP）,albumin（Alb）,albumin/globulin（A/G） ratio,fasting blood glucose,total cholesterol（TC）,total bilirubin（TB）,alkaline phosphatase（ALP）,calcium,phosphorus and so on] were collected,and serum biotin,Vit B12 and FA were determined. Graying severity score（GSS） was used for evaluating patients with premature white hair disease,and the difference of Vit B12 and FA deficiency proportions in different GSS groups was compared. Results There was no statistical significance in sex,age,BMI,TP,Alb,A/G ratio,fasting blood glucose,TC,TB,ALP,calcium and phosphorus levels between the 2 groups（P>0.05）. The levels of biotin,Vit B12 and FA in premature white hair disease group were lower than those in healthy control group（P<0.05）. Among the 80 patients with premature white hair disease,there were 30 cases of GSS mild,42 cases of GSS moderate and 8 cases of GSS severe. There were 10（33.33%）,22（52.38%） and 8（100.00%） cases of Vit B12 deficiency in GSS mild,moderate and severe groups,respectively,and there were 4（13.33%）,13（30.95%） and 4（50.00%） cases of FA deficiency,respectively. The proportions of Vit B12 deficiency and FA deficiency increased with the increase of GSS in patients with premature white hair disease. There was statistical significance for the proportion of Vit B12 deficiency among different GSS groups（P<0.05）,and there was no statistical significance for the proportion of FA deficiency （P>0.05）. Conclusions Serum biotin,Vit B12 and FA are related to premature white hair disease.
