Genotyping research of methicillin resistant Staphylococcus aureus in nosocomial infection

Abstract

Abstract: Objective　To provide scientific referencefor controlling hospital acquired infection through analyzing the genotype homology of methicillin resistant Staphylococcus aureus（MRSA） which were isolated from different departments of hospital. Methods　The genotypes of 23 strains of hospital acquired MRSA were analyzed by repetitive polymerase chain reaction（REP-PCR） and DiversiLab genotype system. Results　Hospital acquired MRSA were classified into 4 kinds of genotypes（A，B，C and D）.A and D types were mainly distributed in Emergency Observation Room，while B and C types were mainly distributed in Traditional Chinese Medicine Surgery Department. Conclusions　B and C types may cause infection outbreak in Traditional Chinese Medicine Surgery Department as epidemic strains. REP-PCR and DiversiLab genotype system can be efficient ways for research of pathogen on nosocomial infection.

Key words: Methicillin-resistant Staphylococcus aureus, Nosocomial infection, Genotyping

QIAO Yun，CHEN Junhao，LUO Yuntao，ZHAO Yingmei，ZHANG Jue. Genotyping research of methicillin resistant Staphylococcus aureus in nosocomial infection[J]. , 2012, 27(12): 1031-1034.

References

[1]Clinical and Laboratory Standards Institute. Performance standardsfor antimicrobial susceptibility testing[S]. M100-S19，CLSI，2009.
[2]Harrington SM，Stockf，Kominski AL，et al. Genotypic analysis of invasive Streptococcus pneumoniaefrom Mali，Africa，by semiautomated repetitive-element PCR and pulsed-field gel electrophoresis[J]. J Clin Microbiol，2007，45（3）：707-714.
[3]Moroney SM，Heller LC，Arbuckle J，et al. Staphy-lococcal cassette chromosome mec and Panton-Valentine leukocidin characterization of methicillin-resistant Staphylococcus aureus clones[J]. J Clin Microbiol，2007，45（3）：1019-1021.
[4]Ross TL，Merz WG，Farkosh M，et al. Comparison of an automated repetitive sequence-based PCR microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus [J]. J Clin Microbiol，2005，43（11）：5642-5647.
[5]Gastmeier P，Sohr D，Geffers C，et al . Occurrence of meticillin-resistant Staphylococcus aureus infections in German intensive care units[J]. Infection，2002，30（4）：198-202.
[6]韦莉萍，靳士英，李春梅，等. 金黄色葡萄球菌医院感染危险因素的研究[J].中华医院感染学杂志，2002，12（3）：175-176.
[7]ShuttCK，Pounder JI，Page SR，et al. Clinical evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for charac-terization of Staphylococcus aureus strains[J]. J Clin Microbiol，2005，43（3）：1187-1192.
[8]Singh A，Goering RV，Simjee S，et al. Application of molecular techniques to the study of hospital infection [J]. Clin Microbiol Rev，2006，19（3）：512-530.
[9]曹晶晶，袁梁，王利君，等.医院感染MRSA的重复序列PCR分型研究[J].中国感染与化疗杂志，2010，10（3）:200-204.
[10]罗柳林，应春妹，倪培华，等. Agilent2100分析仪在鲍曼不动杆菌同源性分析中的应用[J].检验医学，2009，24（7）:481-484.

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