Abstract:

Objective To investigate the distribution of high-risk human papillomavirus （HPV） genotypes in cervical lesion samples. Methods A total of 382 cervical lesion samples were collected for HPV genotyping by molecular rapid diversion hybridization gene chip technology. Results The positive rate of high-risk HPV was 51.3% （196/382）. The positive rates in patients with 19-30,31-40,41-50 and >50 years old were 41.1%,61.1%,56.3% and 43.2%,respectively,but there was no statistical significance for different age groups （P>0.05）. Using cervical intraepithelial neoplasia （CIN） grade or worse as pathological positive diagnosis,there were 154 cases of pathological positive,and the positive rate was 78.6% （154/196） among 196 cases of high-risk HPV positive samples. There were 19 cases of pathological positive among 186 cases of high-risk HPV negative samples with positive rate of 10.2% （19/186）. There was statistical significance between the 2 groups （P<0.05）. A total of 13 subtypes were found from 196 cases of high-risk HPV positive samples. The top 5 subtypes of determination rates from high to low were 16,58,18,52 and 33,however,the positive rates with different subtypes had no statistical significance （P>0.05）. Conclusions High-risk HPV infection is tightly related with the occurrence and development of cervical lesion,and the top 5 subtypes of high-risk HPV infection are 16,58,18,52 and 33 in Xuzhou.

Key words: Human papillomavirus, Genotyping, Cervical lesion